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ID 47702
フルテキストURL
著者
Honda, Junichi Department of Biology, Faculty of Science, Okayama University
Takeuchi, Sakae Department of Biology, Faculty of Science, Okayama University
Fukumachi, Hiroshi Department of Biological Sciences, Graduate School of Science, University of Tokyo
Takahashi, Sumio Department of Biology, Faculty of Science, Okayama University
抄録
Insulin-like growth factor-I (IGF-I) is produced in the liver and other peripheral tissues in response to growth hormone (GH) stimuli. IGF-I regulates diverse physiological functions in an autocrine and/or paracrine manner. IGF-I and IGF-I receptor (type-I receptor) are expressed in human and rat pituitary glands. However, the cell types of IGF-I-expressing cells and target cells of IGF-I in the pituitary glands are not known. The present study was aimed to identify the cell types of IGF-I-expressing cells and of its type-I receptor-expressing cells in mouse pituitary glands. In the mouse pituitary glands, IGF-I mRNA and IGF-I receptor mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR). IGF-I-expressing cells and its receptor-expressing cells were detected by non-radioisotopic in situ hybridization using mouse IGF-I cDNA and IGF-I receptor cDNA probes, and their cell types were immunocytochemically determined using antibodies raised against pituitary hormones. We found that somatotrophs expressed both IGF-I and IGF-I receptors, and some of corticotrophs expressed IGF-I receptors. Co-localization of IGF-I and GH in the same cultured pituitary cells was observed by dual-labelling immunocytochemistry. The present study demonstrated that pituitary IGF-I produced in somatotrophs regulated functions of somatotrophs and corticotrophs in an autocrine and/or paracrine manner.
発行日
1998-08
出版物タイトル
Zoological Science
15巻
4号
出版者
Zoological Society of Japan
出版者(別表記)
日本動物学会
開始ページ
573
終了ページ
579
ISSN
0289-0003
NCID
AA10545874
資料タイプ
学術雑誌論文
言語
English
著作権者
© 1998 Zoological Society of Japan
論文のバージョン
publisher
査読
有り
DOI
PubMed ID
Web of Sience KeyUT