start-ver=1.4 cd-journal=joma no-vol=112 cd-vols= no-issue=1 article-no= start-page=8 end-page=13 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20041020 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Tumor-specific exon creation of the HELLS/SMARCA6 gene in non-small cell lung cancer en-subtitle= kn-subtitle= en-abstract= kn-abstract=In an attempt to identify tumor suppressor genes on chromosome 10 in non-small cell lung cancers, we isolated 10 types of splicing variants of the HELLS/ SMARCA6 gene transcripts. HELLS/SMARCA6 is a novel member of SNF2 family, which is implicated in cellular function like chromatin remodeling. Variant 1 was an alternatively spliced isoform containing an insertion of a 44-ntd intronic sequence between exons 3 and 4, giving rise to a premature termination of translation. The expression of the variant 1 was detected exclusively in the lung cancer specimens (11 of 43 cases, 26%), but was not detected in corresponding normal tissues. D10S520 marker in the proximity of the HELLS/SMARCA6 gene showed prevalent allelic loss (41%) as compared with flanking markers (25-31%). These results suggest that loss of function of HELLS/SMARCA6 by allelic loss and aberrant proteins by tumor-specific exon creation may result in epigenetic deregulation, leading the lung cells to malignancy or its progression. en-copyright= kn-copyright= en-aut-name=YanoMasaaki en-aut-sei=Yano en-aut-mei=Masaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OuchidaMamoru en-aut-sei=Ouchida en-aut-mei=Mamoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ShigematsuHisayuki en-aut-sei=Shigematsu en-aut-mei=Hisayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TanakaNoriyoshi en-aut-sei=Tanaka en-aut-mei=Noriyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=IchimuraKoichi en-aut-sei=Ichimura en-aut-mei=Koichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KobayashiKazuyasu en-aut-sei=Kobayashi en-aut-mei=Kazuyasu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=InakiYasuhiko en-aut-sei=Inaki en-aut-mei=Yasuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=ToyookaShinichi en-aut-sei=Toyooka en-aut-mei=Shinichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TsukudaKazunori en-aut-sei=Tsukuda en-aut-mei=Kazunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=ShimizuNobuyoshi en-aut-sei=Shimizu en-aut-mei=Nobuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=ShimizuKenji en-aut-sei=Shimizu en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil= kn-affil=Department of Surgical Oncology and Thoracic Surgery, Graduate School of Medicine and Dentistry, Okayama University affil-num=2 en-affil= kn-affil=Department of Molecular Genetics, Graduate School of Medicine and Dentistry, Okayama University affil-num=3 en-affil= kn-affil=Department of Surgical Oncology and Thoracic Surgery, Graduate School of Medicine and Dentistry, Okayama University affil-num=4 en-affil= kn-affil=Department of Surgical Oncology and Thoracic Surgery, Graduate School of Medicine and Dentistry, Okayama University affil-num=5 en-affil= kn-affil=Department of Pathology, Graduate School of Medicine and Dentistry, Okayama University affil-num=6 en-affil= kn-affil=Department of Surgical Oncology and Thoracic Surgery, Graduate School of Medicine and Dentistry, Okayama University affil-num=7 en-affil= kn-affil=Department of Surgical Oncology and Thoracic Surgery, Graduate School of Medicine and Dentistry, Okayama University affil-num=8 en-affil= kn-affil=Department of Surgical Oncology and Thoracic Surgery, Graduate School of Medicine and Dentistry, Okayama University affil-num=9 en-affil= kn-affil=Department of Surgical Oncology and Thoracic Surgery, Graduate School of Medicine and Dentistry, Okayama University affil-num=10 en-affil= kn-affil=Department of Surgical Oncology and Thoracic Surgery, Graduate School of Medicine and Dentistry, Okayama University affil-num=11 en-affil= kn-affil=Department of Molecular Genetics, Graduate School of Medicine and Dentistry, Okayama University en-keyword=alternative splicing kn-keyword=alternative splicing en-keyword=HELLS kn-keyword=HELLS en-keyword=loss of heterozygosity kn-keyword=loss of heterozygosity en-keyword=lung cancer kn-keyword=lung cancer en-keyword=SMARCA6 kn-keyword=SMARCA6 END start-ver=1.4 cd-journal=joma no-vol=132B cd-vols= no-issue=1 article-no= start-page=70 end-page=73 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050105 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Association study between brain-derived neurotrophic factor gene polymorphisms and methamphetamine abusers in Japan en-subtitle= kn-subtitle= en-abstract= kn-abstract=Several lines of evidence suggest that genetic factors might contribute to drug abuse vulnerability. Recent genomic scans for association demonstrated that the brain-derived neurotrophic factor (BDNF) gene was associated with drug abuse vulnerability. In this study, we analyzed association of two BDNF gene single nucleotide polymorphisms (SNPs), 132C>T (C270T named formerly) in the noncoding region of exon V and 196G >A (val66met) in the coding region of exon XIIIA, with methamphetamine (MAP) abuse in Japan. No significant differences were found in the frequency of the genotype or allele in these two SNPs between MAP abusers and controls (132C>T in exon V: genotype, p = 0.586, allele, p = 0.594; 196G>A (val66met) in exon XIIIA: genotype, p = 0.889, allele, p = 0.713). Furthermore, there was no difference between clinical parameters (e.g. prognosis psychosis, spontaneous relapse, or poly-substance abuse) and the two SNPs of BDNF gene. These results suggest that the two SNPs (132C>T in exon V and 196G>A (val66met) in exon XIIIA) of the BDNF gene may not be associated with Japanese MAP abusers. en-copyright= kn-copyright= en-aut-name=ItohKanako en-aut-sei=Itoh en-aut-mei=Kanako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HashimotoKenji en-aut-sei=Hashimoto en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ShimizuEiji en-aut-sei=Shimizu en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SekineYoshimoto en-aut-sei=Sekine en-aut-mei=Yoshimoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OzakiNorio en-aut-sei=Ozaki en-aut-mei=Norio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=InadaToshiya en-aut-sei=Inada en-aut-mei=Toshiya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HaranoMutsuo en-aut-sei=Harano en-aut-mei=Mutsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=IwataNakao en-aut-sei=Iwata en-aut-mei=Nakao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KomiyamaTokutaro en-aut-sei=Komiyama en-aut-mei=Tokutaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=YamadaMitsuhiko en-aut-sei=Yamada en-aut-mei=Mitsuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=SoraIchiro en-aut-sei=Sora en-aut-mei=Ichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=NakataKenji en-aut-sei=Nakata en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=UjikeHiroshi en-aut-sei=Ujike en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=IyoMasaomi en-aut-sei=Iyo en-aut-mei=Masaomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= affil-num=1 en-affil= kn-affil=Department of Psychiatry, Chiba University Graduate School of Medicine affil-num=2 en-affil= kn-affil=Department of Psychiatry, Chiba University Graduate School of Medicine affil-num=3 en-affil= kn-affil=Department of Psychiatry, Chiba University Graduate School of Medicine affil-num=4 en-affil= kn-affil=Department of Psychiatry and Neurology, Hamamatsu University School of Medicine affil-num=5 en-affil= kn-affil=Department of Psychiatry and Psychobiology, Nagoya University Graduate School of Medicine affil-num=6 en-affil= kn-affil=Department of Psychiatry and Psychobiology, Nagoya University Graduate School of Medicine affil-num=7 en-affil= kn-affil=Department of Neuropsychiatry, Kurume University School of Medicine affil-num=8 en-affil= kn-affil=Department of Psychiatry, Fujita Health University School of Medicine affil-num=9 en-affil= kn-affil=National Center Hospital for Mental, Nervous and Muscular Disorders, National Center of Neurology and Psychiatry affil-num=10 en-affil= kn-affil=Karasuyama Hospital, Showa University School of Medicine affil-num=11 en-affil= kn-affil=Division of Psychobiology, Tohoku University Graduate School of Medicine affil-num=12 en-affil= kn-affil=Department of Neuropsychiatry, Okayama University Graduate School of Medicine and Dentistry affil-num=13 en-affil= kn-affil=Department of Neuropsychiatry, Okayama University Graduate School of Medicine and Dentistry affil-num=14 en-affil= kn-affil=Department of Psychiatry, Chiba University Graduate School of Medicine en-keyword=brain-derived neurotrophic factor kn-keyword=brain-derived neurotrophic factor en-keyword=polymorphism kn-keyword=polymorphism en-keyword=drug abuse kn-keyword=drug abuse en-keyword=methamphetamine kn-keyword=methamphetamine END start-ver=1.4 cd-journal=joma no-vol=126B cd-vols= no-issue=1 article-no= start-page=43 end-page=45 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20040401 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Association between the Glutathione S-transferase M1 gene deletion and female methamphetamine abusers en-subtitle= kn-subtitle= en-abstract= kn-abstract=Several lines of evidence suggest that increased generation of auto-oxidized dopamine (DA) o-quinone is associated with the neurotoxicity of methamphetamine (MAP) in the brain, and that, as a cellular defenses against DA-derived quinines, glutathione S-transferase (GST) detoxifies auto-oxidized DA o-quinone in the brain. Glutathione S-transferase M1 (GSTM1) of the mu-class of GSTs catalyzes reaction between glutathione and catecholamine o-quinones under physiological conditions. This study was undertaken to investigate the role of the GSTM1 gene deletion polymorphism in the neuropathology of MAP abuse. One hundred fifty-seven MAP abusers and 200 healthy comparison subjects were tested for a genetic polymorphism of GSTM1. The difference in the frequency of deletion (D)/nondeletion (N) alleles between the female abusers and female controls was close to statistical significance (P=0.071), although there was no statistical difference (P=0.651) between male abusers and male controls. Furthermore, the number of female abusers with deletion alleles was significantly (P=0.007, odds ratio: 2.77, 95% CI 1.30-5.89) higher than that of male abusers with deletion alleles. These findings suggest that GSTM1 gene deletion may contribute to a vulnerability to MAP abuse in female subjects, but not in male subjects. (C) 2003 Wiley-Liss, Inc. en-copyright= kn-copyright= en-aut-name=KoizumiHiroki en-aut-sei=Koizumi en-aut-mei=Hiroki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HashimotoKenji en-aut-sei=Hashimoto en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KumakiriChikara en-aut-sei=Kumakiri en-aut-mei=Chikara kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ShimizuEiji en-aut-sei=Shimizu en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SekineYoshimoto en-aut-sei=Sekine en-aut-mei=Yoshimoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OzakiNorio en-aut-sei=Ozaki en-aut-mei=Norio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=InadaToshiya en-aut-sei=Inada en-aut-mei=Toshiya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=HaranoMutsuo en-aut-sei=Harano en-aut-mei=Mutsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KomiyamaTokutaro en-aut-sei=Komiyama en-aut-mei=Tokutaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=YamadaMitsuhiko en-aut-sei=Yamada en-aut-mei=Mitsuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=SoraIchiro en-aut-sei=Sora en-aut-mei=Ichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=UjikeHiroshi en-aut-sei=Ujike en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=TakeiNori en-aut-sei=Takei en-aut-mei=Nori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=IyoMasaomi en-aut-sei=Iyo en-aut-mei=Masaomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= affil-num=1 en-affil= kn-affil=Department of Psychiatry, Chiba University Graduate School of Medicine affil-num=2 en-affil= kn-affil=Department of Psychiatry, Chiba University Graduate School of Medicine affil-num=3 en-affil= kn-affil=Department of Psychiatry, Chiba University Graduate School of Medicine affil-num=4 en-affil= kn-affil=Department of Psychiatry, Chiba University Graduate School of Medicine affil-num=5 en-affil= kn-affil=Department of Psychiatry and Neurology, Hamamatsu University School of Medicine affil-num=6 en-affil= kn-affil=Department of Psychiatry, Fujita Health University School of Medicine affil-num=7 en-affil= kn-affil=Department of Psychiatry, Nagoya University Graduate School of Medicine affil-num=8 en-affil= kn-affil=Department of Neuropsychiatry, Kurume University School of Medicine affil-num=9 en-affil= kn-affil=National Center Hospital for Mental, Nervous, and Muscular Disorders, National Center of Neurology and Psychiatry affil-num=10 en-affil= kn-affil=Karasuyama Hospital, Showa University School of Medicine affil-num=11 en-affil= kn-affil=Division of Psychobiology, Tohoku University Graduate School of Medicine affil-num=12 en-affil= kn-affil=Department of Neuropsychiatry, Okayama University Graduate School of Medicine and Dentistry affil-num=13 en-affil= kn-affil=Department of Psychiatry and Neurology, Hamamatsu University School of Medicine affil-num=14 en-affil= kn-affil=Department of Psychiatry, Chiba University Graduate School of Medicine en-keyword=methamphetamine kn-keyword=methamphetamine en-keyword=drug abuse kn-keyword=drug abuse en-keyword=Glutathione S-transferase kn-keyword=Glutathione S-transferase en-keyword=gender difference kn-keyword=gender difference END start-ver=1.4 cd-journal=joma no-vol=135B cd-vols= no-issue=1 article-no= start-page=5 end-page=9 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050505 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A functional glutathione S-transferase P1 gene polymorphism is associated with methamphetamine-induced psychosis in Japanese population en-subtitle= kn-subtitle= en-abstract= kn-abstract=Several lines of evidence suggest that oxidative stress plays a role in the mechanisms of action of methamphetamine (MAP) in the human brain. Given the role of glutathione S-transferases (GSTs) in the protection against oxidative stress, genes encoding the GSTs have been considered as candidates for association studies of MAP abuse. This study was undertaken to investigate the role of the functional polymorphism of GSTP1 gene exon 5 (Ile105Val) in the pathogenesis of MAP abuse. Genotyping for GSTP1 gene polymorphism exon 5 (Ile105Val) in 189 MAP abusers and 199 normal controls was performed by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). Association between GSTP1 gene polymorphism and clinical features (prognosis of psychosis (transient-type and prolonged-type), spontaneous relapse (positive and negative), and poly-substance abuse) of MAP abusers was evaluated. Significant differences in the frequency of both alleles (P = 0.026, odds ratio: 1.70, 95% confidence intervals (CI) 1.06-2.72) and genotypes (P = 0.029) between MAP abusers and controls were detected. In particular, a significant difference in both genotype frequency (P = 0.013) and allele frequency (P = 0.014, odds ratio: 1.84, 95% CI 1.13-2.97) between MAP abusers with psychosis (transient-type and prolonged-type) and controls was detected. Our findings suggest that the polymorphism (Ile 105Val) on exon 5 of the GSTP1 gene may contribute to a vulnerability to psychosis associated with MAP abuse in Japanese population. en-copyright= kn-copyright= en-aut-name=HashimotoTasuku en-aut-sei=Hashimoto en-aut-mei=Tasuku kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HashimotoKenji en-aut-sei=Hashimoto en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MatsuzakaDaisuke en-aut-sei=Matsuzaka en-aut-mei=Daisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ShimizuEiji en-aut-sei=Shimizu en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SekineYoshimoto en-aut-sei=Sekine en-aut-mei=Yoshimoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YoshiyaInada en-aut-sei=Yoshiya en-aut-mei=Inada kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=IwataNakao en-aut-sei=Iwata en-aut-mei=Nakao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=HaranoMutsuo en-aut-sei=Harano en-aut-mei=Mutsuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KomiyamaTokutaro en-aut-sei=Komiyama en-aut-mei=Tokutaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=YamadaMitsuhiko en-aut-sei=Yamada en-aut-mei=Mitsuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=SoraIchiro en-aut-sei=Sora en-aut-mei=Ichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=UjikeHiroshi en-aut-sei=Ujike en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=IyoMasaomi en-aut-sei=Iyo en-aut-mei=Masaomi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= affil-num=1 en-affil= kn-affil=Department of Psychiatry, Chiba University Graduate School of Medicine affil-num=2 en-affil= kn-affil=Department of Psychiatry, Chiba University Graduate School of Medicine affil-num=3 en-affil= kn-affil=Department of Psychiatry, Chiba University Graduate School of Medicine affil-num=4 en-affil= kn-affil=Department of Psychiatry, Chiba University Graduate School of Medicine affil-num=5 en-affil= kn-affil=Department of Psychiatry and Neurology, Hamamatsu University School of Medicine affil-num=6 en-affil= kn-affil=Department of Psychiatry, Nagoya University Graduate School of Medicine affil-num=7 en-affil= kn-affil=Department of Psychiatry, Fujita Health University School of Medicine affil-num=8 en-affil= kn-affil=Department of Neuropsychiatry, Kurume University School of Medicine affil-num=9 en-affil= kn-affil=National Center Hospital for Mental, Nervous and Muscular Disorders, National Center of Neurology and Psychiatry (NCNP) affil-num=10 en-affil= kn-affil=National Institute of Mental Health, NCNP affil-num=11 en-affil= kn-affil=Division of Psychobiology, Tohoku University Graduate School of Medicine affil-num=12 en-affil= kn-affil=Department of Neuropsychiatry, Okayama University Graduate School of Medicine and Dentistry affil-num=13 en-affil= kn-affil=Department of Psychiatry, Chiba University Graduate School of Medicine en-keyword=methamphetamine kn-keyword=methamphetamine en-keyword=psychosis kn-keyword=psychosis en-keyword=drug abuse kn-keyword=drug abuse en-keyword=genetic factor; polymorphism kn-keyword=genetic factor; polymorphism END