ID | 50975 |
フルテキストURL | |
著者 |
Miki, Yukari
Okayama Univ, Grad Sch Hlth Sci, Dept Med Technol
Gion, Yuka
Okayama Univ, Dept Pathol, Grad Sch Med Dent & Pharmaceut Sci
Mukae, Yuriko
Kawasaki Coll Allied Hlth Profess, Dept Med Technol
Hayashi, Atsushi
Okayama Univ, Grad Sch Hlth Sci, Dept Med Technol
Sato, Hiaki
Okayama Univ, Grad Sch Hlth Sci, Dept Med Technol
Yoshino, Tadashi
Okayama Univ, Dept Pathol, Grad Sch Med Dent & Pharmaceut Sci
Kaken ID
publons
researchmap
Takahashi, Kiyoshi
Okayama Univ, Grad Sch Hlth Sci, Dept Med Technol
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抄録 | Little is known about the S100B+ lymphocytes, which are unique human peripheral blood lymphocytes (PBL) containing the S100B protein. It has recently been shown that S100B is released from various types of S100B+ cells and exhibits varied cytokine-like activities. In this study, we precisely characterized the S100B+ lymphocytes of healthy adults with respect to the proportion in the whole PBL, immunophenotypes, function, and their S100B mRNA expression and also evaluated their S100B-releasing activity upon stimulation. S100B+ lymphocytes were detected in all individuals examined, and the proportion of S100B+ lymphocytes in the whole PBL ranged from 0.42% to 16.15% (mean, 4.21%). In addition, two subtypes of S100B+ lymphocytes, a CTL subtype (CD3+ CD8+ CD16-) and a NK subtype (CD3- CD8- CD16+), were detected. The majority of the CTL subtype of S100B+ lymphocytes expressed the alpha beta-T-cell receptor. Surprisingly, S100B mRNA was detected not only in S100B+ lymphocytes, but also in every S100B- lymphocytes, although the expression levels of S100B mRNA in S100B- lymphocytes were much lower than those of S100B+ lymphocytes. The CTL subtype of S100B+ lymphocytes exhibited blastic morphological changes, proliferated and released S100B upon stimulation with phytohemagglutinin. The NK subtype of S100B+ lymphocytes exhibited morphological NK activity when cocultivated with NK-sensitive target, K-562 cells. Thus, the CTL subtype of S100B+ lymphocytes exhibit the biological characteristics of T cells, while the NK subtype of S100B+ lymphocytes exhibit the characteristics of NK cells. These results suggest that S100B+ lymphocytes are a particular subtype of cytotoxic lymphocytes that play a unique role in antitumor immunity.
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キーワード | S100B
peripheral blood
cytotoxic T cells
NK cells
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発行日 | 2013-02
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出版物タイトル |
European Journal of Haematology
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巻 | 90巻
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号 | 2号
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出版者 | Wiley-Blackwell
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開始ページ | 99
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終了ページ | 110
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ISSN | 0902-4441
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資料タイプ |
学術雑誌論文
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オフィシャル URL | http://dx.doi.org/10.1111/ejh.12036
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関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/50874
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言語 |
英語
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論文のバージョン | author
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査読 |
有り
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DOI | |
Web of Science KeyUT |