ID | 50625 |
フルテキストURL | |
著者 |
Maruo, Tomoko
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol Surg
Ichikawa, Tomotsugu
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol Surg
Kaken ID
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Kanzaki, Hirotaka
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Mol Genet
Inoue, Satoshi
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol Surg
Kurozumi, Kazuhiko
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol Surg
ORCID
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Onishi, Manabu
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol Surg
Yoshida, Koichi
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol Surg
Kambara, Hirokazu
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol Surg
Ouchida, Mamoru
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Mol Genet
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Shimizu, Kenji
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Mol Genet
Tamaru, Seiji
Okayama Univ, Sch Med, Cent Res Lab
Chiocca, E. Antonio
Brigham & Womens Faulkner Hosp, Dept Neurosurg
Date, Isao
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Neurol Surg
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抄録 | One of the insidious biological features of gliomas is their potential to extensively invade normal brain tissue, yet molecular mechanisms that dictate this locally invasive behavior remain poorly understood. To investigate the molecular basis of invasion by malignant gliomas, proteomic analysis was performed using a pair of canine glioma subclones - J3T-1 and J3T-2 - that show different invasion phenotypes in rat brains but have similar genetic backgrounds. Two-dimensional protein electrophoresis of whole-cell lysates of J3T-1 (angiogenesis-dependent invasion phenotype) and J3T-2 (angiogenesis-independent invasion phenotype) was performed. Twenty-two distinct spots were recognized when significant alteration was defined as more than 1.5-fold change in spot intensity between J3T-1 and J3T-2. Four proteins that demonstrated increased expression in J3T-1, and 14 proteins that demonstrated increased expression in J3T-2 were identified using liquid chromatography-mass spectrometry analysis. One of the proteins identified was annexin A2, which was expressed at higher levels in J3T-1 than in J3T-2. The higher expression of annexin A2 in J3T-1 was corroborated by quantitative RT-PCR of the cultured cells and immunohistochemical staining of the rat brain tumors. Moreover, immunohistochemical analysis of human glioblastoma specimens showed that annexin A2 was expressed at high levels in the tumor cells that formed clusters around dilated vessels. These results reveal differences in the proteomic profiles between these two cell lines that might correlate with their different invasion profiles. Thus, annexin A2 may be related to angiogenesis-dependent invasion.
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キーワード | angiogenesis
annexin A2
glioma
invasion
proteomics
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発行日 | 2013-06
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出版物タイトル |
Neuropathology
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巻 | 33巻
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号 | 3号
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出版者 | Wiley-Blackwell
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開始ページ | 264
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終了ページ | 275
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ISSN | 0919-6544
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資料タイプ |
学術雑誌論文
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オフィシャル URL | http://dx.doi.org/10.1111/j.1440-1789.2012.01361.x
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関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/50652
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言語 |
英語
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論文のバージョン | author
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査読 |
有り
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DOI | |
Web of Science KeyUT |