ID | 52861 |
フルテキストURL | |
著者 |
Inoue, Kentaro
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Med & Clin Sci
Wada, Jun
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Med & Clin Sci
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Kaken ID
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Eguchi, Jun
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Med & Clin Sci
Kaken ID
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Nakatsuka, Atsuko
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Med & Clin Sci
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Teshigawara, Sanae
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Med & Clin Sci
Kaken ID
Murakami, Kazutoshi
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Med & Clin Sci
ORCID
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Ogawa, Daisuke
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Med & Clin Sci
Kaken ID
Terami, Takahiro
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Med & Clin Sci
Katayama, Akihiro
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Med & Clin Sci
Tone, Atsuhito
Natl Hosp Org, Okayama Med Ctr, Dept Diabet & Metab
Iseda, Izumi
Natl Hosp Org, Okayama Med Ctr, Dept Diabet & Metab
Hida, Kazuyuki
Natl Hosp Org, Okayama Med Ctr, Dept Diabet & Metab
Yamada, Masao
GlycoTechnica Ltd
Ogawa, Tomohisa
GP BioSci Co Ltd
Makino, Hirofumi
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Med & Clin Sci
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抄録 | We analyzed the urine samples of patients with type 2 diabetes at various stages of diabetic nephropathy by lectin microarray to identify a biomarker to predict the progression of diabetic nephropathy. Japanese patients with type 2 diabetes at various stages of nephropathy were enrolled and we performed lectin microarray analyses (n = 17) and measured urinary excretion of fetuin-A (n = 85). The increased signals of urine samples were observed in Sia alpha 2-6Gal/GalNAc-binding lectins (SNA, SSA, TJA-I) during the progression of diabetic nephropathy. We next isolated sialylated glycoproteins by using SSA-lectin affinity chromatography and identified fetuin-A by liquid chromatography-tandem mass spectrometer. Urinary excretion of fetuin-A significantly increased during the progression of albuminuria (A1, 0.40 +/- 0.43; A2, 0.60 +/- 0.53; A3 1.57 +/- 1.13 ng/gCr; p = 7.29x10(-8)) and of GFR stages (G1, 0.39 +/- 0.39; G2, 0.49 +/- 0.45; G3, 1.25 +/- 1.18; G4, 1.34 +/- 0.80 ng/gCr; p = 3.89x10(-4)). Multivariate logistic regression analysis was employed to assess fetuin-A as a risk for diabetic nephropathy with microalbuminuria or GFR<60 mL/min. Fetuin-A is demonstrated as a risk factor for both microalbuminuria and reduction of GFR in diabetic nephropathy with the odds ratio of 4.721 (1.881-11.844) and 3.739 (1.785-7.841), respectively. Collectively, the glycan profiling analysis is useful method to identify the urine biomarkers and fetuin-A is a candidate to predict the progression of diabetic nephropathy.
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発行日 | 2013-10-15
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出版物タイトル |
PLoS ONE
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巻 | 8巻
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号 | 10号
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出版者 | Public Library Science
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ISSN | 1932-6203
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資料タイプ |
学術雑誌論文
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関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/52832
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言語 |
英語
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著作権者 | © 2013 Inoue et al.
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論文のバージョン | publisher
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査読 |
有り
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DOI | |
Web of Science KeyUT |