start-ver=1.4 cd-journal=joma no-vol=21 cd-vols= no-issue=1 article-no= start-page=225 end-page=227 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=201101 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Antisense effect of pyrrolidine-based oxy-peptide nucleic acids in Escherichia coli en-subtitle= kn-subtitle= en-abstract= kn-abstract=To investigate the antisense effect of a pyrrolidine-based oxy-peptide nucleic acid (POPNA), we carried out the LacZ reporter assay using a 12-mer trans-l-POPNA conjugated with a cell-penetrating peptide (antisense reagent). The antisense effect of the conjugated POPNA (inhibition of LacZ activity) was comparable to that shown by a Nielsen-type peptide nucleic acid. Furthermore, the conjugated POPNA could switch the LacZ activity over a wide range of ambient temperatures. en-copyright= kn-copyright= en-aut-name=KitamatsuMizuki en-aut-sei=Kitamatsu en-aut-mei=Mizuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KuramiShunsuke en-aut-sei=Kurami en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OhtsukiTakashi en-aut-sei=Ohtsuki en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SisidoMasahiko en-aut-sei=Sisido en-aut-mei=Masahiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Department of Medical and Bioengineering, Graduate School of Natural Science and Technology affil-num=2 en-affil= kn-affil=Department of Medical and Bioengineering, Graduate School of Natural Science and Technology affil-num=3 en-affil= kn-affil=Department of Medical and Bioengineering, Graduate School of Natural Science and Technology affil-num=4 en-affil= kn-affil=Department of Medical and Bioengineering, Graduate School of Natural Science and Technology en-keyword=Peptide nucleic acid kn-keyword=Peptide nucleic acid en-keyword=Cell-penetrating peptide kn-keyword=Cell-penetrating peptide en-keyword=Antisense effect kn-keyword=Antisense effect END