JaLCDOI 10.18926/AMO/32786
フルテキストURL fulltext.pdf
著者 Yamamoto, Goki| Oda, Takuzo|
抄録

For the purpose to clarify the distribution of DNA in mouse ascites sarcoma cells (SR-C3H) induced by Rous sarcoma virus (Schmidt-Ruppin strain), quantitative assays were carried out by SCHMIDT-THANNHAUSERSCHNEIDER'S method using subcellular fractions isolated from SR-C3H cells and C3H mouse liver as a control tissue, and simultaneously electron microscopic observations were conducted with the rotary shadowed preparations of the SDS-phenol extracted nucleic acids by the protein monolayer technique. The results are briefly summarized as follows. 1. The RNA/DNA ratios in SR-C3H cells and liver cells were 2.3 and 3.7, while those in nuclear fraction of SR-C3H cells and liver cells were 0.34 and O. 56, respectively. The electron micrographs of nuclear nucleic acids revealed a DNA-RNA complex-like structure. 2. DNA and RNA contents of SR-C3H mitochondria were found to be 3.1 and 24 fl-g per mg of protein, respectiVely, which proved to be greater than those of liver mitochondria. The mean values of the contour length of circular DNA molecules in highest frequency group observed in the electron micrographs were 4.88 μ. in SR-C3H mitochondria and 5.08 μ. in mouse liver mitochondria. There could be observed circular molecules of duplicated-length in both mitochondrial DNA's and small circular molecules in SR-C3H mitochondrial DNA. 3. In the microsomal and supernatant fractions of SR-C3H cells and mouse liver cells, the ratios of DNA to RNA gave several percent by chemical analysis and this percentage was particularly high in the supernatant of SR-C3H cells. On the other hand, in the electron micrographs, the fibrous structure was significantly recovered in the supernatant nucleic acids of SR-C3H cells, but with difficulty in the other three fractions. This fibrous structure measured 1.13 μ in the mean value of the length and was considered to be DNA as it readily disappeared after the treat· ment with DNase.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-06
24巻
3号
出版者 Okayama University Medical School
開始ページ 287
終了ページ 302
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4321319
NAID 120002312108
JaLCDOI 10.18926/AMO/32787
フルテキストURL fulltext.pdf
著者 Szirmai, Endre| Celander, David Robert| Szantay, Janos| Celander, Evelyn|
抄録

Die Autoren haben uber ihre Erfahrungen mit dem neuen Glutisal- Versuchspraparat - einer Kombination von Salicylamid und Dimethyl- aminophenyldimethylpyrazolon im Zusammenhang mit der Behandlung von 350 Patienten berichtet. Es wurden vor und wahrend der Behandlung instrumentell die Schmerzempfindlichkeit, die Funktion der quergestreiften Muskulatur (Tonus und Kontraktionsamplitude), Kreislauf, Nerven- und Gelenkfunk· tion mit dem Myotonometer und Angioneuromyographen (nach SZIRMAI), Oszillometer, Oszillographen, Angiometer registriert. Es wurde die Harn- saureausscheidung bei Gichtfallen registriert. Die Autoren fanden, daβ das Praparat die Erkaltungen, verschiedene rheumatische Erkrankungen sowie Gicht gunstig beeinflusst. Die Vertra- glichkeit war sehr gut.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-06
24巻
3号
出版者 Okayama University Medical School
開始ページ 355
終了ページ 363
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4249891
NAID 120002311528
JaLCDOI 10.18926/AMO/32788
フルテキストURL fulltext.pdf
著者 Tsunemitsu, Kensuke|
抄録

1. The mixed lymphocyte culture test where live lymphocytes of female mice are cultured with supersonicated cell homogenate of isogeneic male mice does not reflect the difference in H-Y histocompatibility antigen alone. 2. When non-H-2 antigens are cumulated in various combinations, the rate of blastformation becomes greater than the combination of C3H ♀ themselves. 3. When non-H-2 antigens and H-Y antigens are added in various combinations, the rate of blastformation becomes significantly higher than in the combination of C3H ♀ themselves.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-06
24巻
3号
出版者 Okayama University Medical School
開始ページ 303
終了ページ 310
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4249886
NAID 120002311391
JaLCDOI 10.18926/AMO/32789
フルテキストURL fulltext.pdf
著者 Ogata, Masana| Kumashiro, Kazuo|
抄録

The effect of thioinosine on antibody response of immunized rabbits has been studied. When given simultaneously with the antigen, thioinosine profoundly suppresses the formation of humoral antibody.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-06
24巻
3号
出版者 Okayama University Medical School
開始ページ 311
終了ページ 314
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4249887
NAID 120002311795
JaLCDOI 10.18926/AMO/32790
フルテキストURL fulltext.pdf
著者 Yamamoto, Shinichiro| Omura, Sachiko| Oda, Takuzo|
抄録

The correlation of infectivity to the length of SV 40 DNA by dilute and undiluted passage was described. DNA was extracted from purified virions by Marmur's method, and propagation of virus was done using VERO cells. The infectivity of SV 40 (simian virus 40) ran parallel with the length of its DNA. Undiluted passage caused shortening of DNA and decrease in infectivity, but when these undiluted group was passaged dilutely, length of DNA approached the original length and the infectivity recovered. In undiluted passage group small circular DNAs under 1.0,11_, so far not reported in the SV 40 DNA, were found in low frequency. Replicating form and dimers were also noted from virions and the nuclei of SV 40 infected VERO cells.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-06
24巻
3号
出版者 Okayama University Medical School
開始ページ 273
終了ページ 285
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4321318
NAID 120002311449
JaLCDOI 10.18926/AMO/32791
フルテキストURL fulltext.pdf
著者 Ueda, Masatoshi| Taketa, Kazuhisa|
抄録

1. Twenty.one patients with liver diseases were studied for their urinary mehylmalonic acid excretion after a valine load by means of an improved thin layer chromatography. 2. Methylmalonic acid positive cases were found in four out of the ten patients with cirrhosis of the liver, all four with cirrhosis and diabetes mellitus, and none with acute hepatitis of icteric phase. No apparent correlation was found between the methylmalonic acid excretion and the extent of hepatic damage. 3. A large amount of methylmalonic acid found in the case (S. I.) with cirrhosis of the liver and diabetes mellitus after the valine load was not corrected by cyanocobalamin but by DBCC, suggesting an impaired transformation from cyanocobalamin to DBCC. However, the nature of the impairment remains unknown.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-06
24巻
3号
出版者 Okayama University Medical School
開始ページ 365
終了ページ 372
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4249892
NAID 120002311363
JaLCDOI 10.18926/AMO/32792
フルテキストURL fulltext.pdf
著者 Ogata, Masana| Osaki, Hirokazu|
抄録

For the purpose of forecasting the prevalence ofJapanese encephalitis in Japan, we tried to find out the correlation of factors between median and mode dates of epidemic time curve of prevalence on one hand, and average atmospheric temperatures of prefectures in June and July (T6,7 in short) (X¹), the time when HI reaction of swine became positive to the degree of 50 per cent (D. pos. swine in short) (X²), the latitude (x³) and longitude (x4) in respective prefectures (in 1965 and 1967). On the other we also estimated the median and mode dates of this epidemic curve of the prevalence in 1968 and 1969, from the regression equation of one variable and multiple regression equation from the above factors using an electronic computer. The usefulness of adding factors concerned with mosquitoes to the above four factors is proven by the accuracy of estimation. And the following results were obtained. 1) Phenomenally speaking, the prevalence of Japanese encephalitis follows the principle of "advancing of prevalence towards the north and east" and essentially speaking, it depends upon high atmospheric temperature and the outbreak of many hazardous mosquitoes by the high atmospheric temperature. 2) To estimate median date (y) and mode rate (z) of the epidemic time curve of the prevalence, we can use the next equations; The regression equations to estimate y and z from T 6,7(X) are as follows. y = - 3. 75X¹ + 144.47 σ = 12.4.·. [1] z = - 3. 80X¹ + 157 .26 σ = 14.9.. · [1]' The regression equation from D. pos. swine (X²) are as follows. y = 0. 68X² + 31. 82 σ = 9.2· .. [2] z=0. 76X² +40. 71 σ= 12.0 .. · [2]' The multiple regression equation from T6 ,7 and D. pos. swme are as follows. y = -1. 07X¹ +0 .62x² +59. 37 σ= 9.7 ... [3] z= -0. 79x¹ +0. 71x² +61.02 σ= 12.0· .. [3]' The multiple regression equations from T 6•7, D. pos. swine, latitude and longitude are as follows Y= -1.01x¹ +0.58x² -0.26x³+0 .37x4 + 18.50 σ= 9.8・・・ [4] z = -0. 32x¹ +0. 52x² +2 .05x³ +0 .54x4 -87. 81 σ= 11.8 [4]' 3) We Obtained the estimated value of median date in 17 prefectures in Kyushu, Chugoku, Shikoku, Kinki and Kanto provinces in 1968 and in 13 prefectures in 1969 from [l] or [2] or [3] or [4] equation. Nine prefectures out of 17 by [l], 12 prefectures by [2], 13 by [3J and [4] in 1968. [4] could be estimated with about 10 days error or less. And in 1969, 9 out of 13 by [3] and 7 out of 13 by [4] could be accurately esti· mated. The estimation by the multiple regression equation using many factors is most useful for the calculation. 4) The time when the number of patients increases at maximum can be pointed out by the lower limit of prediction region obtained from data in each prefecture. And the lower limit was the estimated median value minus about 20 days by [1] and about 16 days by [2] or [3] or [4] under the next condition; α = 0. 1, N= 75. 5) The mode dates in 17 prefectures out of 19 were estimated by [1]', [2]', [3]' and [4]'. 12 prefectures out of 17 by [1]', 7 by [2]', 10 by [3]' and 13 by [4]' could be estimated with about 12 days error or less in 1968 and 9 out of 13 was correctly estimated by [3]' and [4]' in 1969. The estimation by the regression line of one factor was s~mewhat different from each other, but when multiple regression line of four factors was used the estimation became more correct. Judging from these results, it is adequate to use the multiple regression equation of [4] and [4]' when we want to forecast the median date or mode date ofJapanese encephalitis time cure. 6) In the case of adding two factors concerned with mosquitoes to T6,7 (X¹), D. pos. swine (x²), latitude (x³), longitude (x4), multiple regression equations become as follows. y= -1.46x¹+0.14X²+0.068x5+89.03 σ= 6.9.. ·[5] z= -3. 29x¹+0 .13x²-0. 010x5+ 143.63 σ= 18.6··· [5]' y=-4.20x¹+0.35x²+0.29x6 + 53.70 σ= 4.2 .. ·[6] z=-2.56x¹-0.0lx²-0.02x6 +128.96 σ=11.4 [6]' y= 4.76x¹+0.41x²+0.13x5+0.22x6-72.78 σ= 4.5 [7] z = - 2. l0x¹ + 0. 05x²+ 0. 11 x5 - 0. 08x6+ 113.4 σ= 10. 7.. · [7]' where x5 is the time when the number of mosquitoes (C. T. collected by light trap reached the maximum and X6 is the time when hazardous mosq uitoes were dected. In the case of median date, 5 prefectures out of 6 prefectures by [5], 2 out of 6 by [6] and 2 out of 5 by [7], and in the case of mode date, 5 out of 6 by [5]', 4 out of 5 by [6]' and 4 out of 5 by [7]' could be accurately estimated in 1969.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-06
24巻
3号
出版者 Okayama University Medical School
開始ページ 343
終了ページ 353
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4395193
NAID 120002312246
JaLCDOI 10.18926/AMO/32793
フルテキストURL fulltext.pdf
著者 Tomokuni, Katsumaro|
抄録

Female Cb mice weighing 20-23 g were exposed to 800 ppm (in average) of 1, I, 2, 2.tetrachloroethane for 3 hours. Both triglyceride and phospholipid in the liver and plasma were determined at varying times after the exposure. On the other hand, there were observed the ultraviolet absorption spectra of microwmallipids in the liver at 90 minutes after the 1, 1,2, 2-tetrachloroethane or the carbon tetrachloride exposure. The results thus obtained are summarized as follows: 1. The increase of hepatic triglyceride contents attained the maximum level in the period between 20 and 25 hours after the exposure and declined to the initial levels at 90 hours later. 2. The plasma triglyceride levels decreased until 25 hours after the exposure, then tended to increase significantly and were much higher than the control levels in the period between 70 and 90 hours later. 3. Both liver and plasma phospholipid levels decreased gradually up to 25 hours after the exposure, then slowly recovered with almost the same rate of increase. 4. It was suggested that the inhalation of the above vapors induced a little change in microsomal lipids in the liver.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-06
24巻
3号
出版者 Okayama University Medical School
開始ページ 315
終了ページ 322
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4249888
NAID 120002311753
JaLCDOI 10.18926/AMO/32794
フルテキストURL fulltext.pdf
著者 Masuji, Hiroshi|
抄録

1) Normal karyotype of Donryu strain rat was determined according to the classification of KURITA et al. (8). Namely, the number of chromosomes was 42 in diploid cells, and chromosomes were divided into 3 groups (A, B and C) according to the position of centromere. A.group was consisted of 7 pairs of metacentric chromosomes, B-group 4 pairs of sub· meta.subtelocentrics and C.group 10 pairs of telocentrics and Y. 2) Among all chromosome pairs a pair of the longest telocentric chromosomes (C.l), 4 pairs of all the B.group, and the Y chromosome were recognizable. 3) The presence of polymorphism was demonstrated in the smallest submetacentric chromosomes (BA),: namely, (I) a homologous submeta. centric pair, (II) a homologous subtelocentric pair and (III) a heteromor. phic submeta and subtelocentric pair which seemed to be a hybrid from (I) and (II). To distinguish the polymorphism in their genotype from phenotype was impossible. 4) Animals with type III B·4 chromosomes were produced by type I and type II animals. 5) By checking the chromosomes of the inbred Donryu strain rats maintained over 40 generations by brother.sister mating at Nihon Rat Co., polymorphism in BA chromosomes was also recognized.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-02
24巻
1号
出版者 Okayama University Medical School
開始ページ 81
終了ページ 91
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4246451
NAID 120002312269
JaLCDOI 10.18926/AMO/32795
フルテキストURL fulltext.pdf
著者 Ogata, Masana| Kumashiro, Kazuo|
抄録

Localization of IgM and IgG sypylitic antibodies in the sera of patients and the experimental syphylitic rabbits was examined by the gel filtration on Sephadex G.200 column. I) In the case of late syphylitic patients; OGATA test-reactive antibodies were contained in IgM and IgG fractions. On the other hand, RPCF test-reactive antibody was contained only in IgG fraction. This discrepancy may be due to the difference in antigens; Cardiolipin.resicin and T. P. Reiter protein. 2) In the case of the experimental syphylitic rabbits; The results were as follows. a) Variation in the level of the titer. The peaks of the titer were seen 3-4 weeks after inoculation of T. P. Nichols by OGATA test, VDRL test and RPCF test, thereafter titers decreased. On the other hand, the titer kept on rising up to 2 months and maintained high level during the periods of 3, 4 and 5 months by TPHA test. b)Transformation of antibody from IgM to IgG. Transformation of antibody from IgM to IgG was seen 3-4 weeks after inoculation by all four tests; OGATA test, VDRL test, RPCF test and TPHA test, and such a transformation was completed 3 months after inoculation.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-02
24巻
1号
出版者 Okayama University Medical School
開始ページ 93
終了ページ 99
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4246832
NAID 120002311720
JaLCDOI 10.18926/AMO/32796
フルテキストURL fulltext.pdf
著者 Inoue, Masanao|
抄録

For the purpose to reveal the changes in the metabolism of erythroblast in varied specialization stages the author observed the Feulgen DNA level of rabbit erythroblasts by microspectrophotometry. Observations were made on normal and anemic animals, and those receiving a mass red cell transfusion at the recovery stage of anemia where the early denucleation is stimulated. Observations have revealed that in normal erythropoiesis the DNA contents are kept at n to 2 n level from the proerythroblast to late basophilic stage, but in later stages, polychromatic and orthochromatic, DNA level per cell decreases gradually with advance of the cell specialization reaching the minimum level, nearly haploid level, at orthochromatic stage where most cells are believed to be denucleated. In blood depleted animals nearly the same pattern of DNA level was observed in connection with erythroid specialization as that in normal animal, except a relatively high DNA level in the later specialization. In the cases of the hemolytic anemia a similar tendency has been observed but the minimum level of DNA remains at a higher level, hypo-diploid level, in poly- and orthochromatic stages. Twenty-four hours after the mass red cell transfusion by which severe anemia has been recovered to the original level within one hour, the pattern of the DNA level of the erythroblast returns to the normal one showing a very low DNA level at the polyand the orthochromatic stages. The data indicate that the DNA synthesis of erythroblast kept at n to 2 n levels until the late basophilic stage begins to decline at polychromatic stage and reaches nearly haploid level at orthochromatic stage, but in active hemopoiesis the DNA synthesis is stimulated and the DNA contents are kept at a high level even in the late specialization stages, showing no relation between the denucleation and the low DNA level.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-02
24巻
1号
出版者 Okayama University Medical School
開始ページ 15
終了ページ 26
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4246448
NAID 120002311376
JaLCDOI 10.18926/AMO/32797
フルテキストURL fulltext.pdf
著者 Kodama, H.| Fujii, Y.| Mizuhara, S.| Tanaka, K.|
Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-02
24巻
1号
出版者 Okayama University Medical School
開始ページ 107
終了ページ 109
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4246447
NAID 120002311749
JaLCDOI 10.18926/AMO/32798
フルテキストURL fulltext.pdf
著者 Sogabe, Koiti|
抄録

For the purpose to obtain the information of the mechanism of protein uptake by the tumor cells, some cytochemical and electron microscopic observations were carried out on Ehrlich ascites tumor cells incubated with horseradish peroxidase (basic hemoprotein, molecular weight approximately 40,000) in vitro. In the earlier periods of the incubation peroxidase was found to be adsorbed on some area of surface of the tumor cells forming a thin protein layer, where an active pseudopodia formation was observed. With the lapse of time, the protein was taken in the deep cytoplasm by the infoldings of the cell membrane and accumulated in the cytoplasmic vesicles having limiting membrane. Concerning the accumulation of the protein into the vesicles, small tubular structures in the cytoplasm connecting the cell surface and the vesicles, were considered to participate in the intracellular transportation of peroxidase taken up. In cold environment (2°C), the formation of pseudopodia and deep inward infoldings of the cell membrane was inhibited and simultaneously the uptake of peroxidase stopped. Iodoacetate and sodium fluoride also effected to suppress the pseudopodia and infoldings formation moderetely, as well as uptake of peroxidase, though they did not stop completely. These facts have indicated that horseradish peroxdase is taken up by Ehrlich ascites tuimor cells through pinocytosis which involves energy-requiring process dependent upon glycolytic metabolism of the tumor cells.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-02
24巻
1号
出版者 Okayama University Medical School
開始ページ 27 27 27
終了ページ 48 48 48
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4246830
NAID 120002311948
JaLCDOI 10.18926/AMO/32799
フルテキストURL fulltext.pdf
著者 Yamamoto, Goki| Oda, Takuzo| Tsukamoto, Hiromichi|
抄録

With the purpose to clarified the mode of localization and mechanisms of activation of ATPase in the mitochondrial membrane, analyses were made on the properties of mitochondrial ATPase from the structural and functional aspects. The activation of ATPase by DNP and Mg++ and the oligomycin sensitivity were investigated in a series of inner membrane fragment samples obtained by ultrasonic irradiation and those samples obtained in the processes of isolation and purification of ATPase from rat liver mitochondria and beef heart mitochondria in parallel with electron microscope observations. As a result it has been found that the membrane fragments obtained from rat liver and beef heart mitochondria by ultrasonication exhibited high respiratory activity and unmasked ATPase activity which was charac· terized by remarkable stimulation by Mg++ and inhibition by oligomycin and azide. Therefore, mitochondrial ATPase seems to be bound fairly closely to the inner mitochondrial membrane. In the membrane fragments prepared by ultrasonication of intact mitochondria, ATPase activity was stimulated by DNP, but in the supernatant fractions was not. On the other hand, the supernatant fraction obtained from BHM and inner membrane fragments by severe sonication exhibits a marked ATPase activity and the activity incresed in each step of the purification on the treatments with acid, protamine and heat. Especially in the case of membrane fragments the protamine treatment can be omitted. Electron microscope observation of the fractions in each step of the purification proved the head pieces to be ATPase. The ATPase activity of solubilized head pieces is insensitive to oligo. mycin and coincides with the soluble ATPase of PULLMAN etat. (8) in the points of its cold labile property and optimum pH, but it shown no accele. ration of ATPase activity by DNP.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-02
24巻
1号
出版者 Okayama University Medical School
開始ページ 49
終了ページ 64
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4246831
NAID 120002311871
JaLCDOI 10.18926/AMO/32800
フルテキストURL fulltext.pdf
著者 Miyahara, Masanobu|
抄録

The role of -SH groups in mitochondrial energy transfer reaction was studied by observing the reduction of a disulphide, 5, 5'-dithiobis (2-nitrobenzoic acid), DTNB, a specific analytical agent for the estimation of -SH groups in biological materials, by addition of it to the isolated rat liver mitochondria in various respiratory states, as defined by CHANCE and WILLIAMS. 1. In the various respiratory states, states 1 to 5, the reduction of DTNB proceeds most rapidly at state 5, and most slowly at state 3. DTNB reduction at state 5 is suppressed by the partial oxidation of respiratory carriers with oxygen (state 4) and the addition of respiratory substrate does not affect the DTNB reduction. 2. The retardation in the reduction rate at state 3 is relieved partially by a respiratory inhibitor, KCN, and is intensified markedly by oligomycin, an inhibitor of oxidative phosphorylation. An uncoupler for oxidative phosphorylation, DNP, does not affect the reduction rate at state 3. At state 4 the reduction is stimulated by DNP and KCN, but is unaffected by oligomycin. The results suggest that the alteration in the functions of the energy transfer reaction in mitochondria is accompanied by changes in the occurrence and the functioning of -SH groups which can be detected by the reactivity with DTNB. The data suggest also that there are at least two kinds of -SH groups reacting with DTNB: the one is the -SH group which reacts DTNB actively when the respiratory carriers are kept reduced, and the other is the one which reacts actively when the respiratory carriers are kept oxidized, participating in the phosphorylating system and its reactivity with DTNB diminishes in the actively phosphorylating states (states 2 and 3).

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-02
24巻
1号
出版者 Okayama University Medical School
開始ページ 1 1 1
終了ページ 13 13 13
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4246449
NAID 120002311379 120002311379
JaLCDOI 10.18926/AMO/32801
フルテキストURL fulltext.pdf
著者 Szirmai, Endre| Royl, Peter| Wesser, Ullrich|
抄録

Eine Salieyl-Chinin-Lithium-Kombination wurde im Siemens Unterriehtsreaktor SUR 100 BE des Instituts fur Kernteehnik der Teehnisehen UniversiUit Berlin mit Neutronen aktiviert. AnsehlieBend erfolgte die Messung der spektralen Verteilung der Gamma-Zahlrate zu zwei versehiedenen Zeitpunkten naeh der Aktivierung. Es wird festgestellt, daB diese Salieyl-Chinin-Lithium-Kombination nur sehwaeh im SUR-Reaktor aktivierbar ist, vergliehen mit einer Goldsonde etwa 200 mal sehwaeher. Trotzdem konnten mit Hilfe eines 400-Kanal-Analysators die spektralen Verteilungen der Gamma-Energien dieses Praparates noch sehr genau bestimmt werden. Ein ausgepragter Gamma-Peak tritt auf bei der Energie 0.53 MeV. Er klingt ab mit einer Halbwertszeit von 1.6 h und ist vermut· lich auf ein Element der Tablettierhilfsstoffe zurUckzufUhren. Vom strahlenphysikalischen Gesichtspunkt ist das Praparat fUr die Human. medizin gut geeignet.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-02
24巻
1号
出版者 Okayama University Medical School
開始ページ 101
終了ページ 105
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4246446
NAID 120002312232
JaLCDOI 10.18926/AMO/32802
フルテキストURL fulltext.pdf
著者 Miwa, Hiroaki|
抄録

1) It has been found that the peripheral blood of hybrid adult dogs contains about 29.4 % of lymphocytes in average. When such a blood is passed through the glass wool column the leucocytes containing 68-90 % (81.6% in average) lymphocytes are obtained. 2) In the single culture of such lymphocytes alone and mixed culture both live lymphocytes and sonicated lymphocytes in the presence of 1% (vIv) PHA, the peak of the blastformation of lymphocytes is observed at culture hour 72. 3) In the abscence of PHA both single culture and mixed culture of lymphocytes show hardly any blastformation. 4) In the mixed culture of live lymphocytes with homogenate of sonicated lymphocytes with addition of 1% (vIv) PHA the rate of blast. formation observable at culture hour 72 and the rejection of kidney trans· plant 7 days after its transplantation of hybrid adult dogs show a direct correlation, demonstrating that the mixed lymphocyte reaction reflects accurately the difference in donor histocompatibility antigens against the recipient.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-02
24巻
1号
出版者 Okayama University Medical School
開始ページ 65
終了ページ 80
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4246450
NAID 120002312099
JaLCDOI 10.18926/AMO/32803
フルテキストURL fulltext.pdf
著者 yamane, Takashi| Matsuo, Toshihiko| Hasebe, Satoshi| Ohtsuki, Hiroshi|
抄録

The purpose of this study was to elucidate the role of extracellular matrix components such as aggrecan, fibronectin, and laminin in the extraocular muscle of patients with strabismus. Resected tissues of the medial rectus muscle of 47 patients with intermittent exotropia obtained during recession-resection surgery were frozen under liquid nitrogen and pulverized by a Freezer/Mill to solubilize the tissue for enzyme immunoassay. The total amounts of aggrecan, fibronectin, and laminin in the resected tissue were correlated with clinical data of patients such as age, exodeviation, and refractive error. The amount of aggrecan decreased significantly with the advance of age (P < 0.0001, Spearman rank correlation test), while the amount of laminin or fibronectin had no correlation with age. Patients with basic type intermittent exotropia showed larger, although not significantly, amounts of aggrecan than those with convergence insufficiency type (P = 0.0538, Mann-Whitney U-test). The amount of aggrecan may be related to motor aspects of intermittent exotropia.

キーワード extraocular muscle aggrecan laminin fibronectin intermittent extropia
Amo Type Article
出版物タイトル Acta Medica Okayama
発行日 2003-08
57巻
4号
出版者 Okayama University Medical School
開始ページ 199
終了ページ 204
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 14627072
Web of Science KeyUT 000184987100006
JaLCDOI 10.18926/AMO/32804
フルテキストURL fulltext.pdf
著者 Aktekin, Mustafa| Kurtoglu, Zeliha| Ozturk, Ahmet Hakan|
抄録

We observed a bilateral and symmetrical variation of the anterior belly of the digastric muscle during the dissection of a 35-year-old female cadaver. The accessory muscle bundles were arranged in a cross. These bundles were found superficial to the mylohyoid muscle and deep in the platysma. Such a variation from perfect symmetry has not been previously reported. To avoid misinterpretation of radiological tests, it is important to be aware of bilateral and symmetrical variations of the anterior belly of the digastric muscle when examining the floor of the mouth and the submental region.

キーワード digastric muscle anterior belly variation
Amo Type Article
出版物タイトル Acta Medica Okayama
発行日 2003-08
57巻
4号
出版者 Okayama University Medical School
開始ページ 205
終了ページ 207
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 14627073
Web of Science KeyUT 000184987100007
JaLCDOI 10.18926/AMO/32805
フルテキストURL fulltext.pdf
著者 Matsuka, Yoko| Wang, Da-Hong| Suganuma, Narufumi| Imai, Kaori| Ikeda, Satoru| Taketa, Kazuhisa| Kira, Shohei|
抄録

We studied the association of gamma-glutamyltransferase (GGT) and other serum markers of liver injury with daily alcohol consumption in a healthy population of 1,043 Japanese males. A positive correlation between daily alcohol consumption and biochemical markers, such as log GGT (r = 0.432), log AST (r = 0.244) or log LAP (r = 0.246), was seen in all drinkers. However, there was a negative correlation, such as log GGT (r = -0.434), log AST (r = -0.424) or log LAP (r = -0.430), in heavy drinkers who consumed more than 70 g ethanol a day. On the other hand, a positive correlation, such as log GGT (r = 0.426), log AST (r = 0.247) or log LAP (r = 0.216) was found in moderate drinkers who consumed less than 70 g ethanol a day. Interestingly, there was a tendency toward negative association between alcohol consumption and the Tokyo University ALDH2 Phenotype Screening Test (TAST) score in the heavy drinkers, and there was a tendency toward positive association between GGT and TAST score in this group. Our results suggest that there are 2 groups of drinkers, those with elevated GGT (good responders) and those with normal GGT (poor responders) despite heavy drinking.

キーワード gamma-glutamyltransferase(GGT) daily alcohol consumption good responder and poor responder
Amo Type Article
出版物タイトル Acta Medica Okayama
発行日 2003-08
57巻
4号
出版者 Okayama University Medical School
開始ページ 171
終了ページ 178
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 14627068
Web of Science KeyUT 000184987100002