ID | 60634 |
フルテキストURL | |
著者 |
Saeki, Nozomu
Graduate School of Environmental and Life Science, Okayama University
Eguchi, Yuichi
Center for Mechanisms of Evolution, School of Life Sciences, Arizona State University
Kintaka, Reiko
Donnelly Center for Cellular and Biomolecular Research, Department of Medical Genetics, University of Toronto
Makanae, Koji
Graduate School of Environmental and Life Science, Okayama University
Shichino, Yuichi
RNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research
Iwasaki, Shintaro
RNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research
Kanno, Manabu
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology
Kimura, Nobutada
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology
Moriya, Hisao
Research Core for Interdisciplinary Sciences, Okayama University
ORCID
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抄録 | Extreme overproduction of gratuitous proteins can overload cellular protein production resources, leading to growth defects, a phenomenon known as the protein burden/cost effect. Genetic screening in the budding yeast Saccharomyces cerevisiae has isolated several dubious ORFs whose deletions mitigated the protein burden effect, but individual characterization thereof has yet to be delineated. We found that deletion of the YJL175W ORF yielded an N-terminal deletion of Swi3, a subunit of the SWI/SNF chromatin remodeling complex, and partial loss of function of Swi3. The deletion mutant showed a reduction in transcription of genes encoding highly expressed, secreted proteins and an overall reduction in translation. Mutations in the chromatin remodeling complex could thus mitigate the protein burden effect, likely by reallocating residual cellular resources used to overproduce proteins. This cellular state might also be related to cancer cells, as they frequently harbor mutations in the SWI/SNF complex.
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キーワード | Cell growth
Gene expression
Gene regulation
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発行日 | 2020-06-11
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出版物タイトル |
Scientific Reports
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巻 | 10巻
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号 | 1号
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出版者 | Nature
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開始ページ | 9500
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ISSN | 2045-2322
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © The Author(s) 2020
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
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関連URL | isVersionOf https://doi.org/10.1038/s41598-020-66307-z
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ライセンス | http://creativecommons.org/licenses/by/4.0/
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助成機関名 |
日本学術振興会
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助成番号 | 15KK0258
17H03618
18K19300
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