ID | 57772 |
フルテキストURL | |
著者 |
Yamashita, Toru
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
ORCID
Kaken ID
researchmap
Shang, Jingwei
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Nakano, Yumiko
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Morihara, Ryuta
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Sato, Kota
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
ORCID
Takemoto, Mami
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
ORCID
Kaken ID
Hishikawa, Nozomi
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
Ohta, Yasuyuki
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
researchmap
Abe, Koji
Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kaken ID
publons
researchmap
|
抄録 | The therapeutic effect of in vivo direct reprogramming on ischemic stroke has not been evaluated. In the present study, a retroviral solution (1.5-2.0 × 107 /ul) of mock pMX-GFP (n = 13) or pMX-Ascl1/Sox2/NeuroD1 (ASN) (n = 14) was directly injected into the ipsilateral striatum and cortex 3 days after 30 min of transient cerebral ischemia. The reprogrammed cells first expressed neuronal progenitor marker Dcx 7 and 21 days after viral injection, then expressed mature neuronal marker NeuN. This was accompanied by morphological changes, including long processes and synapse-like structures, 49 days after viral injection. Meanwhile, therapeutic improvement was not detected both in clinical scores or infarct volume. The present study provides a future novel self-repair strategy for ischemic stroke with beneficial modifications of the inducer-suppressor balance.
|
発行日 | 2019-07-29
|
出版物タイトル |
Scientific Reports
|
巻 | 9巻
|
号 | 1号
|
出版者 | NPG
|
開始ページ | 10956
|
ISSN | 2045-2322
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
OAI-PMH Set |
岡山大学
|
著作権者 | © The Author(s) 2019
|
論文のバージョン | publisher
|
PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1038/s41598-019-47482-0
|
ライセンス | http://creativecommons.org/licenses/by/4.0/
|
Citation | Yamashita, T., Shang, J., Nakano, Y. et al. In vivo direct reprogramming of glial linage to mature neurons after cerebral ischemia. Sci Rep 9, 10956 (2019) doi:10.1038/s41598-019-47482-0
|
助成機関名 |
文部科学省
|
助成番号 | 17H04196
17K10827
15K15527
|