Japan Society for Bioscience, Biotechnology, and AgrochemistryActa Medica Okayama0916-84517142007Synthesis of lipid derivatives of pyrrole polyamide and their biological activity10781082ENMasahikoYamamotoChangjinZhuLuiYiZhengRongYoshieMiuraMinoruIzumiShuheiNakajimaKen-ichiTanamotoSakayuShimizuNaomichiBabaNovel fatty acyl and phospholipid derivatives of pyrrole polyamide were synthesized. Their cytotoxicity against a cancer cell line of MT-4 cells and those infected by human immunodeficiency virus (HIV) was examined. Although no anti-HIV activity was found, their cytotoxicitty against the cancer cells was significantly enhanced by introducing a lipophilic group into the pyrrole polyamide.No potential conflict of interest relevant to this article was reported.Japan Society for Bioscience, Biotechnology, and AgrochemistryActa Medica Okayama0916-84517132007Glucosylation of Sucrose Laurate with Cyclodextrin Glucanotransferase826829ENKatsuhideOkadaHaisuoZhaoMinoruIzumiShuheiNakajimaNaomichiBabaSucrose monolauroyl esters were found to serve as substrates for cyclodextrin glucanotransferase (CGTase)-catalyzed transglucosidation reactions, affording new sucrose esters that have an additional 1-3 glucose residues on the pyranose ring of the sucrose moiety in the ester.No potential conflict of interest relevant to this article was reported.Japan Society for Bioscience, Biotechnology, and AgrochemistryActa Medica Okayama0916-84517142007Three antinematodal diterpenes from Euphorbia kansui10861089ENJian-XiaoShiZhi-XuanLiTeruhikoNitodaMinoruIzumiHiroshiKanzakiNaomichiBabaKazuyoshiKawazuShuheiNakajimaThree compounds, 20-O-acetyl-[3-O-(2'E,4'Z)-deca-dienoyl]-ingenol (1), 20-O-acetyl-[5-O-(2'E,4'Z)-decadienoyl]-ingenol (2) and 3-O-(2'E,4'Z)-decadienoylingenol (3), were isolated from Euphorbia kansui under the bioassay-guided method. Each compound showed the same antinematodal activity against the nematode, Bursaphelenchus xylophilus, at a minimum effective dose (MED) of 5 mu g/cotton ball.No potential conflict of interest relevant to this article was reported.Japan Society for Bioscience, Biotechnology, and AgrochemistryActa Medica Okayama0916-84517142007Evidence for new beta 1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera11111114ENYoshinobuKimuraShoSakamuraTakayukiUshijimaYoichiroHamaHiroyukiKajiuraKazuhitoFujiyamaKiyoshiOkiharaKenHashimotoHiroyukiSugimotoHideoYamadaIn a previous study (Y. Kimura et al., Biosci. Biotechnol Biochem., 70, 2583-2587, 2006), we found that new complex type N-glycans harboring Thomsen-Friedenreich antigen (Ga1 beta 1-3GalNAc) unit occur on royal jelly glycoproteins, suggesting the involvement of a new beta 1-3galactosyltransferase in the synthesis of the unusual complex type N-glycans. So far, such beta 1-3galactosyltransferase activity, which can transfer galactosyl residues with the beta 1-3 linkage to beta 1-4 GalNAc residues in N-glycan, has not been found among any eucaryotic cells. But using GalNAc(2)GlcNAc(2)Man(3)-GlcNAc(2)-PA as acceptor N-glycan, we detected the beta 1-3 galactosyltransferase activity in membrane fraction prepared from honeybee cephalic portions. This result indicates that honeybee expresses a unique beta 1-3 galactosyltransferase involved in biosynthesis of the unusual N-glycan containing a tumor related antigen in the hypopharyngeal gland.No potential conflict of interest relevant to this article was reported.