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ID 48703
フルテキストURL
著者
Shen, Jian-Ren Graduate School of Natural Science and Technology, Okayama University
Kawakami, Keisuke Graduate School of Natural Science and Technology, Okayama University
Koike, Hiroyuki Department of Biosciences, Faculty of Science and Engineering, Chuo University
抄録
This chapter describes the purification and crystallization of oxygen-evolving photosystem II core dimer complex from a thermophilic cyanobacterium Thermosynechococcus vulcanus. Procedures used for purification of photosystem II from the cyanobacterium involves cultivation of cells, isolation of thylakoid membranes, purification of crude and pure photosystem II core complexes by detergent solubilization, followed by differential centrifugation and column chromatography. The purified core dimer particles were successfully used for crystallization, and the methods and conditions used for crystallization are presented. These purification and crystallization procedures can be applied for another thermophilic cyanobacterium T. elongatus.
キーワード
Photosystem II
Oxygen evolution
Crystallization
Membrane proteins
Ion-exchange chromatography
発行日
2011
出版物タイトル
Methods in Molecular Biology
684巻
出版者
Humana Press
開始ページ
41
終了ページ
51
ISSN
1064-3745
NCID
AA11067778
資料タイプ
学術雑誌論文
プロジェクト
エネルギー環境新素材拠点
オフィシャル URL
http://www.springerlink.com/content/t782187342578257/
言語
English
著作権者
© Springer Science+Business Media, LLC
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DOI
PubMed ID