start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=73
end-page=88
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=20140916
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Detection and analysis of non-retroviral RNA virus-like elements in plant, fungal, and insect genomes.
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Endogenous non-retroviral RNA like sequences (NRVSs) have been discovered in the genome of a wide range of eukaryotes. These are considered as fossil RNA viral elements integrated into host genomes by as-yet-known mechanisms, and in many cases, those fossils are estimated to be millions-of-years-old. It is likely that the number of NRVS records will increase rapidly due to the growing availability of whole-genome sequences for many kinds of eukaryotes. Discovery of the novel NRVSs and understanding of their phylogenetic relationship with modern viral relatives provide important information on deep evolutionary history of RNA virus-host interactions. In this chapter, therefore, the common strategies for the identification and characterization of endogenous NRVSs from plants, insects, and fungi are described.
en-copyright=
kn-copyright=

en-aut-name=KondoHideki
en-aut-sei=Kondo
en-aut-mei=Hideki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=ChibaSotaro
en-aut-sei=Chiba
en-aut-mei=Sotaro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=SuzukiNobuhiro
en-aut-sei=Suzuki
en-aut-mei=Nobuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=Institute of Plant Science and Resources (IPSR), Okayama University
kn-affil=

affil-num=2
en-affil=Institute of Plant Science and Resources (IPSR)Okayama University
kn-affil=

affil-num=3
en-affil=Institute of Plant Science and Resources (IPSR), Okayama University
kn-affil=
en-keyword=Pareovirology
kn-keyword=Pareovirology
en-keyword=Molecular fossil record
kn-keyword=Molecular fossil record
en-keyword=Non-retrovirus-like sequence
kn-keyword=Non-retrovirus-like sequence
en-keyword=Database search
kn-keyword=Database search
en-keyword=Whole-genome shotgun
kn-keyword=Whole-genome shotgun
en-keyword=Genomic PCR
kn-keyword=Genomic PCR
en-keyword=Southern blotting
kn-keyword=Southern blotting
en-keyword=Phylogenetic analysis
kn-keyword=Phylogenetic analysis
en-keyword=Maximum-likelihood
kn-keyword=Maximum-likelihood
END

start-ver=1.4
cd-journal=joma
no-vol=684
cd-vols=
no-issue=
article-no=
start-page=41
end-page=51
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2011
dt-pub=2011
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Purification and Crystallization of Oxygen-Evolving Photosystem II Core Complex from Thermophilic Cyanobacteria
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This chapter describes the purification and crystallization of oxygen-evolving photosystem II core dimer complex from a thermophilic cyanobacterium Thermosynechococcus vulcanus. Procedures used for purification of photosystem II from the cyanobacterium involves cultivation of cells, isolation of thylakoid membranes, purification of crude and pure photosystem II core complexes by detergent solubilization, followed by differential centrifugation and column chromatography. The purified core dimer particles were successfully used for crystallization, and the methods and conditions used for crystallization are presented. These purification and crystallization procedures can be applied for another thermophilic cyanobacterium T. elongatus.
en-copyright=
kn-copyright=

en-aut-name=ShenJian-Ren
en-aut-sei=Shen
en-aut-mei=Jian-Ren
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=KawakamiKeisuke
en-aut-sei=Kawakami
en-aut-mei=Keisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=KoikeHiroyuki
en-aut-sei=Koike
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

affil-num=1
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University

affil-num=2
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University

affil-num=3
en-affil=
kn-affil=Department of Biosciences, Faculty of Science and Engineering, Chuo University
en-keyword=Photosystem II
kn-keyword=Photosystem II
en-keyword=Oxygen evolution
kn-keyword=Oxygen evolution
en-keyword=Crystallization
kn-keyword=Crystallization
en-keyword=Membrane proteins
kn-keyword=Membrane proteins
en-keyword=Ion-exchange chromatography
kn-keyword=Ion-exchange chromatography
END