フルテキストURL J_Orthop_Sci_22_4_715.pdf fig.pdf
著者 Lu, Zhichao| Furumatsu, Takayuki| Fujii, Masataka| Maehara, Ami| Ozaki, Toshifumi|
抄録 BACKGROUND: The meniscus plays an important role in controlling the complex biomechanics of the knee. Meniscus injury is common in the knee joint. The perimeniscal capillary plexus supplies the outer meniscus, whereas the inner meniscus is composed of avascular tissue. Angiogenesis factors, such as vascular endothelial growth factor (VEGF), have important roles in promoting vascularization of various tissues. VEGF-mediated neovascularization is beneficial to the healing of injured tissues. However, the distribution and angiogenic role of VEGF remains unclear in the meniscus and injured meniscus. We hypothesized that VEGF could affect meniscus cells and modulate the meniscus healing process. METHODS: Menisci were obtained from total knee arthroplasty patients. Meniscal injury was created ex vivo by a microsurgical blade. VEGF mRNA and protein expression were detected by the polymerase chain reaction and immunohistochemical analyses, respectively. RESULTS: In native meniscal tissue, the expression of VEGF and HIF-1α mRNAs could not be detected. However, VEGF and HIF-1α mRNAs were found in cultured meniscal cells (VEGF: outer > inner; HIF-1α: outer = inner). Injury increased mRNA levels of both VEGF and HIF-1α, with the increase being greatest in the outer area. Immunohistochemical analyses revealed that VEGF protein was detected mainly in the outer region and around injured areas of the meniscus. However, VEGF concentrations were similar between inner and outer menisci-derived media. CONCLUSIONS: This study demonstrated that both the inner and outer regions of the meniscus contained VEGF. HIF-1α expression and VEGF deposition were high in injured meniscal tissue. Our results suggest that injury stimulates the expression of HIF-1α and VEGF that may be preserved in the extracellular matrix as the healing stimulator of damaged meniscus, especially in the outer meniscus.
キーワード vascular endothelial growth factor (VEGF) meniscus meniscal injury hypoxia-inducible factor-1α (HIF-1α)
備考 This is an Accepted Manuscript of an article published by Elsevier
発行日 2017-07
出版物タイトル Journal of Orthopaedic Science
22巻
4号
出版者 Elsevier
開始ページ 715
終了ページ 721
ISSN 0949-2658
NCID AA11052566
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
PubMed ID 28318650
DOI 10.1016/j.jos.2017.02.006
Web of Sience KeyUT 000407394700022
関連URL isVersionOf https://doi.org/10.1016/j.jos.2017.02.006
フルテキストURL Adv_Math_305_144.pdf
著者 Hashimoto, Mitsuyasu| Symondsb, Peter|
抄録 We define the Frobenius limit of a module over a ring of prime characteristic to be the limit of the normalized Frobenius direct images in a certain Grothendieck group. When a finite group acts on a polynomial ring, we calculate this limit for all the modules over the twisted group algebra that are free over the polynomial ring; we also calculate the Frobenius limit for the restriction of these to the ring of invariants. As an application, we generalize the description of the generalized F-signature of a ring of invariants by the second author and Nakajima to the modular case.
キーワード Frobenius direct image Hilbert–Kunz multiplicity F-signature Frobenius limit
備考 This is an Accepted Manuscript of an article published by Elsevier
発行日 2017-01-10
出版物タイトル Advances in Mathematics
305巻
出版者 Elsevier
開始ページ 144
終了ページ 164
ISSN 0001-8708
NCID AA00513055
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
DOI 10.1016/j.aim.2016.09.020
Web of Sience KeyUT 000406169200003
関連URL isVersionOf https://doi.org/10.1016/j.aim.2016.09.020
著者 Sing’ombe Ombiro Geofrey| Sawai, Taku| Noutoshi, Yoshiteru| Nishina, Yuta| Matsui, Hidenori| Yamamoto, Mikihiro| Toyoda, Kazuhiro| Ichinose, Yuki|
抄録 Plant pathogenic bacteria cause huge yield losses in crops globally. Therefore, finding effective bactericides to these pathogens is an immediate challenge. In this study, we sought compounds that specifically inhibit the growth of Ralstonia solanacearum. As a result, we identified one promising compound, 1-(4-bromophenyl)-6-methoxy-2,3,4,9-tetrahydro-1H-β-carboline, which inhibited the growth of R. solanacearum (Rs1002) from a pilot library of 376 chemicals provided from RIKEN. We further obtained its structural analogues and assessed their ability to inhibit Rs1002 growth. Then we identified five compounds, named ralhibitins A to E, that specifically inhibit growth of Rs1002 at >5 μg/ml final concentration. The most effective compounds, ralhibitins A, C, and E completely inhibited the growth of Rs1002 at 1.25 μg/ml. In addition, ralhibitins A to E inhibited growth of Xanthomonas oryzae pv. oryzae but not the other bacteria tested at a final concentration of 10 μg/ml. Whereas, ralhibitin E, besides inhibiting R. solanacearum and X. oryzae pv. oryzae, completely inhibited the growth of X. campestris pv. campestris and the Gram-positive bacterium Clavibacter michiganensis subsp. michiganensis at 10 μg/ml. Growth inhibition by these compounds was stable at pH 6–9 and after autoclaving. Because Rs1002 grew in the culture medium in which ralhibitins were incubated with the ralhibitin-insensitive bacteria, the unaffected bacteria may be able to inactivate the inhibitory effect of ralhibitins. These results suggest that ralhibitins might be potential lead compounds for the specific control of phytopathogenic bacteria.
キーワード Bactericide Ralhibitins Ralstonia solanacearum Lead compounds
備考 This is an Accepted Manuscript of an article published by Elsevier This fulltext will be available in June 2019
発行日 2018-10
出版物タイトル Microbiological Research
215巻
出版者 Elsevier
開始ページ 29
終了ページ 35
ISSN 0944-5013
NCID AA11017780
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
DOI 10.1016/j.micres.2018.06.005
関連URL isVersionOf https://doi.org/10.1016/j.micres.2018.06.005
著者 Matsuura, Koji| Takenami, Mami| Kuroda, Yuka| Hyakutake, Toru| Yanase, Shinichiro| Naruse, Keiji|
発行日 2012-01
出版物タイトル Reproductive BioMedicine Online
24巻
1号
資料タイプ 学術雑誌論文
フルテキストURL BBRC_485_2_261.pdf
著者 Kawaguchi, Yuka| Nariki, Hiroaki| Kawamoto, Naoko| Kanehiro, Yuichi| Miyazaki, Satoshi| Suzuki, Mari| Magari, Masaki| Tokumitsu, Hiroshi| Kanayama, Naoki|
抄録  Activation-induced cytidine deaminase (AID) is essential for diversification of the Ig variable region (IgV). AID is excluded from the nucleus, where it normally functions. However, the molecular mechanisms responsible for regulating AID localization remain to be elucidated. The SR-protein splicing factor SRSF1 is a nucleocytoplasmic shuttling protein, a splicing isoform of which called SRSF1-3, has previously been shown to contribute to IgV diversification in chicken DT40 cells. In this study, we examined whether SRSF1-3 functions in IgV diversification by promoting nuclear localization of AID. AID expressed alone was localized predominantly in the cytoplasm. In contrast, co-expression of AID with SRSF1-3 led to the nuclear accumulation of both AID and SRSF1-3 and the formation of a protein complex that contained them both, although SRSF1-3 was dispensable for nuclear import of AID. Expression of either SRSF1-3 or a C-terminally-truncated AID mutant increased IgV diversification in DT40 cells. However, overexpression of exogenous SRSF1-3 was unable to further enhance IgV diversification in DT40 cells expressing the truncated AID mutant, although SRSF1-3 was able to form a protein complex with the AID mutant. These results suggest that SRSF1-3 promotes nuclear localization of AID probably by forming a nuclear protein complex, which might stabilize nuclear AID and induce IgV diversification in an AID C-terminus-dependent manner.
キーワード AID DT40 Gene conversion Ig SRSF1 Somatic hypermutation
発行日 2017-04
出版物タイトル Biochemical and Biophysical Research Communications
485巻
2号
出版者 Elsevier
開始ページ 261
終了ページ 266
ISSN 0006-291X
NCID AA00564395
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
PubMed ID 28235482
DOI 10.1016/j.bbrc.2017.02.097
Web of Sience KeyUT 000396798300007
関連URL isVersionOf https://doi.org/10.1016/j.bbrc.2017.02.097
フルテキストURL K0005427_other1.pdf
著者 Okano, Mitsuhiro| Fujiwara, Tazuko| Kariya, Shin| Higaki, Takaya| Makihara, Sei-ichiro| Haruna, Takenori| Noyama, Yasuyuki| Koyama, Takahisa| Omichi, Ryotaro| Orita, Yorihisa| Miki, Kentaro| Kanai, Kengo| Nishizaki, Kazunori|
抄録 BACKGROUND: Toll-like receptor 3 (TLR3) is expressed in upper airways, however, little is known regarding whether Toll-like receptor 3 (TLR3) signals exert a regulatory effect on the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP), especially on eosinophilic inflammation. We sought to investigate the effect of Poly(IC), the ligand for TLR3, on cytokine production by dispersed nasal polyp cells (DNPCs). METHODS: DNPCs were pretreated with or without Poly(IC), and were then cultured in the presence or absence of staphylococcal enterotoxin B (SEB), following which the levels of IL-5, IL-10, IL-13, IL-17A and interferon (IFN)-γ in the supernatant were measured. To determine the involvement of IL-10 and cyclooxygenase in Poly(IC)-mediated signaling, DNPCs were treated with anti-IL-10 monoclonal antibody and diclofenac, the cyclooxygenase inhibitor, respectively. Poly(IC)-induced prostaglandin E2 (PGE2) production was also determined. RESULTS: Exposure to Poly(IC) induced a significant production of IL-10, but not of IL-5, IL-13, IL-17A or IFN-γ by DNPCs. Pretreatment with Poly(IC) dose-dependently inhibited SEB-induced IL-5, IL-13 and IL-17A, but not IFN-γ production. Neutralization of IL-10 significantly abrogated the inhibitory effect of Poly(IC). Treatment with diclofenac also abrogated the inhibitory effect of Poly(IC) on SEB-induced IL-5 and IL-13 production. However, unlike exposure of diclofenac-treated DNPCs to lipopolysaccharide, the ligand for TLR4, exposure of these cells to Poly(IC) did not enhance IL-5 or IL-13 production. Poly(IC) did not significantly increase PGE2 production by DNPCs. CONCLUSIONS: These results suggest that TLR3 signaling regulates eosinophilia-associated cytokine production in CRSwNP, at least in part, via IL-10 production.
キーワード Chronic rhinosinusitis with nasal polyps IL-10 IL-5 Poly(IC) Toll-like receptor
備考 学位審査副論文
発行日 2016-01
出版物タイトル Allergology international : official journal of the Japanese Society of Allergology
65巻
1号
出版者 Elsevier
開始ページ 96
終了ページ 102
ISSN 1323-8930
NCID AA11091750
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン publisher
PubMed ID 26666485
Web of Sience KeyUT 000367238600015
関連URL https://doi.org/10.1016/j.alit.2015.08.005 http://ousar.lib.okayama-u.ac.jp/54846
フルテキストURL J_Orthop_Sci_21_4_524.pdf fig.pdf
著者 Furumatsu, Takayuki| Maehara, Ami| Ozaki, Toshifumi|
抄録 BACKGROUND: Proper functioning of the meniscus depends on the composition and organization of its fibrocartilaginous extracellular matrix. We previously demonstrated that the avascular inner meniscus has a more chondrocytic phenotype compared with the outer meniscus. Inhibition of the Rho family GTPase ROCK, the major regulator of the actin cytoskeleton, stimulates the chondrogenic transcription factor Sry-type HMG box (SOX) 9-dependent α1(II) collagen (COL2A1) expression in inner meniscus cells. However, the crosstalk between ROCK inhibition, SOX9, and other transcription modulators on COL2A1 upregulation remains unclear in meniscus cells. The aim of this study was to investigate the role of SOX9-related transcriptional complex on COL2A1 expression under the inhibition of ROCK in human meniscus cells. METHODS: Human inner and outer meniscus cells were prepared from macroscopically intact lateral menisci. Cells were cultured in the presence or absence of ROCK inhibitor (ROCKi, Y27632). Gene expression, collagen synthesis, and nuclear translocation of SOX9 and Smad2/3 were analyzed. RESULTS: Treatment of ROCKi increased the ratio of type I/II collagen double positive cells derived from the inner meniscus. In real-time PCR analyses, expression of SOX9 and COL2A1 genes was stimulated by ROCKi treatment in inner meniscus cells. ROCKi treatment also induced nuclear translocation of SOX9 and phosphorylated Smad2/3 in immunohistological analyses. Complex formation between SOX9 and Smad3 was increased by ROCKi treatment in inner meniscus cells. Chromatin immunoprecipitation analyses revealed that association between SOX9/Smad3 transcriptional complex with the COL2A1 enhancer region was increased by ROCKi treatment. CONCLUSIONS: This study demonstrated that ROCK inhibition stimulated SOX9/Smad3-dependent COL2A1 expression through the immediate nuclear translocation of Smad3 in inner meniscus cells. Our results suggest that ROCK inhibition can stimulates type II collagen synthesis through the cooperative activation of Smad3 in inner meniscus cells. ROCKi treatment may be useful to promote the fibrochondrocytic healing of the injured inner meniscus.
備考 This is an Accepted Manuscript of an article published by Elsevier
発行日 2016-07
出版物タイトル Journal of Orthopaedic Science
21巻
4号
出版者 Elsevier
開始ページ 524
終了ページ 529
ISSN 0949-2658
NCID AA11052566
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
PubMed ID 27113646
DOI 10.1016/j.jos.2016.02.013
Web of Sience KeyUT 000381142800020
関連URL isVersionOf https://doi.org/10.1016/j.jos.2016.02.013
著者 Yoshioka-Nishimura, Miho| Yamamoto, Yasusi|
発行日 2014
出版物タイトル Journal of Photochemistry and Photobiology B: Biology
137巻
資料タイプ 学術雑誌論文
著者 Yan, He| Li, Lin| Alam, M. Jahangir| Shinoda, Sumio| Miyoshi, Shin-ichi| Shi, Lei|
発行日 2010-10-15
出版物タイトル International Journal of Food Microbiology
143巻
3号
資料タイプ 学術雑誌論文
著者 Okazaki, Hiroyuki| Yoshida, Rikiya| Iwai, Keisuke| Noami, Kengo| Muro, Takayuki| Nakamura, Tetsuya| Wakita, Takanori| Muraoka, Yuji| Hirai, Masaaki| Tomioka, Fumiaki| Takano, Yoshihiko| Takenaka, Asami| Toyoda, Masahiro| Oguch, Tamio| Yokoya, Takayoshi|
発行日 2010-12
出版物タイトル Physica C-Superconductivity and its Applications
470巻
S1号
資料タイプ 学術雑誌論文
著者 Nishioka, Yasutaka| Suzumori, Koichi| Kanda, Takefumi| Wakimoto, Shuichi|
発行日 2010-12
出版物タイトル Sensors and Actuators A: Physical
164巻
1-2号
資料タイプ 学術雑誌論文
著者 Khan, Shahbaz Manzoor| Debnath, Chanchal| Pramanik, Amiya Kumar| Xiao, Lihua| Nozaki, Tomoyoshi| Ganguly, Sandipan|
発行日 2011-06-10
出版物タイトル Veterinary Parasitology
178巻
3-4号
資料タイプ 学術雑誌論文
著者 Khan, Shahbaz Manzoor| Debnath, Chanchal| Pramanik, Amiya Kumar| Xiao, Lihua| Nozaki, Tomoyoshi| Ganguly, Sandipan|
発行日 2010-07-15
出版物タイトル Veterinary Parasitology
171巻
1-2号
資料タイプ 学術雑誌論文
著者 Nohara, Minoru| Kakiya, Satomi| Kudo, Kazutaka| Oshiro, Yoshihiro| Araki, Shingo| Kobayashi, Tatsuo C.| Oku, Kenta| Nishibori, Eiji| Sawa, Hiroshi|
発行日 2012-04
出版物タイトル Solid State Communications
152巻
8号
資料タイプ 学術雑誌論文
著者 Miyoshi, Shin-ichi| Abe, Yuki| Senoh, Mitsutoshi| Mizuno, Tamaki| Maehara, Yoko| Nakao, Hiroshi|
発行日 2011-05
出版物タイトル Toxicon
57巻
6号
資料タイプ 学術雑誌論文
著者 Matsuura, Koji| Hayashi, Nobuyoshi| Kuroda, Yuka| Takiue, Chisato| Hirata, Rei| Takenami, Mami| Aoi, Yoko| Yoshioka, Nanako| Habara, Toshihiro| Mukaida, Tetsunori| Naruse, Keiji|
発行日 2010-03
出版物タイトル Reproductive BioMedicine Online
20巻
3号
資料タイプ 学術雑誌論文
著者 Ghosh, Souvik| Gatheru, Zipporah| Nyangao, James| Adachi, Noriaki| Urushibara, Noriko| Kobayashi, Nobumichi|
発行日 2011-01
出版物タイトル Infection, Genetics and Evolution
11巻
1号
資料タイプ 学術雑誌論文
フルテキストURL K0005273 other2.pdf
著者 Sakamoto, Shinji| Takaki, Manabu| Okahisa, Yuko| Mizuki, Yutaka| Kodama, Masafumi| Ujike, Hiroshi| Uchitomi, Yosuke|
備考 学位審査副論文
発行日 2014-05
出版物タイトル Psychiatry Research
216巻
2号
出版者 Elsevier
開始ページ 288
終了ページ 289
ISSN 0165-1781
NCID AA0036432X
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
PubMed ID 24576429
DOI 10.1016/j.psychres.2014.02.006
Web of Sience KeyUT 000335099900020
関連URL https://doi.org/10.1016/j.psychres.2014.02.006
著者 Hashimoto, Mitsuyasu|
抄録 Using the description of the Frobenius limit of modules over the ring of invariants under an action of a finite group on a polynomial ring over a field of characteristic p>0 developed by Symonds and the author, we give a characterization of the ring of invariants with a positive dual F-signature. Combining this result and Kemper's result on depths of the ring of invariants under an action of a permutation group, we give an example of an F-rational, but non-F-regular ring of invariants under the action of a finite group.
キーワード F-rational F-regular Dual F-signature Frobenius limit
備考 This is an Accepted Manuscript of an article published by Elsevier This fulltext available in Aug 2019
発行日 2017-08-15
出版物タイトル Journal of Algebra
484巻
出版者 Elsevier
開始ページ 207
終了ページ 223
ISSN 0021-8693
NCID AA00692420
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
DOI 10.1016/j.jalgebra.2017.04.017
Web of Sience KeyUT 000403626100010
関連URL https://doi.org/10.1016/j.jalgebra.2017.04.017
著者 Wajima, Takeaki| Sabui, Subrata| Fukumoto, Megumi| Kano, Shigeyuki| Ramamurthy, Thandavarayan| Chatterjee, Nabendu Sekhar| Hamabata, Takashi|
発行日 2011-10
出版物タイトル Microbial Pathogenesis
51巻
4号
資料タイプ 学術雑誌論文