フルテキストURL J_Orthop_Sci_21_4_524.pdf fig.pdf
著者 Furumatsu, Takayuki| Maehara, Ami| Ozaki, Toshifumi|
抄録 BACKGROUND: Proper functioning of the meniscus depends on the composition and organization of its fibrocartilaginous extracellular matrix. We previously demonstrated that the avascular inner meniscus has a more chondrocytic phenotype compared with the outer meniscus. Inhibition of the Rho family GTPase ROCK, the major regulator of the actin cytoskeleton, stimulates the chondrogenic transcription factor Sry-type HMG box (SOX) 9-dependent α1(II) collagen (COL2A1) expression in inner meniscus cells. However, the crosstalk between ROCK inhibition, SOX9, and other transcription modulators on COL2A1 upregulation remains unclear in meniscus cells. The aim of this study was to investigate the role of SOX9-related transcriptional complex on COL2A1 expression under the inhibition of ROCK in human meniscus cells. METHODS: Human inner and outer meniscus cells were prepared from macroscopically intact lateral menisci. Cells were cultured in the presence or absence of ROCK inhibitor (ROCKi, Y27632). Gene expression, collagen synthesis, and nuclear translocation of SOX9 and Smad2/3 were analyzed. RESULTS: Treatment of ROCKi increased the ratio of type I/II collagen double positive cells derived from the inner meniscus. In real-time PCR analyses, expression of SOX9 and COL2A1 genes was stimulated by ROCKi treatment in inner meniscus cells. ROCKi treatment also induced nuclear translocation of SOX9 and phosphorylated Smad2/3 in immunohistological analyses. Complex formation between SOX9 and Smad3 was increased by ROCKi treatment in inner meniscus cells. Chromatin immunoprecipitation analyses revealed that association between SOX9/Smad3 transcriptional complex with the COL2A1 enhancer region was increased by ROCKi treatment. CONCLUSIONS: This study demonstrated that ROCK inhibition stimulated SOX9/Smad3-dependent COL2A1 expression through the immediate nuclear translocation of Smad3 in inner meniscus cells. Our results suggest that ROCK inhibition can stimulates type II collagen synthesis through the cooperative activation of Smad3 in inner meniscus cells. ROCKi treatment may be useful to promote the fibrochondrocytic healing of the injured inner meniscus.
備考 This is an Accepted Manuscript of an article published by Elsevier
発行日 2016-07
出版物タイトル Journal of Orthopaedic Science
21巻
4号
出版者 Elsevier
開始ページ 524
終了ページ 529
ISSN 0949-2658
NCID AA11052566
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
PubMed ID 27113646
DOI 10.1016/j.jos.2016.02.013
Web of Sience KeyUT 000381142800020
関連URL isVersionOf https://doi.org/10.1016/j.jos.2016.02.013
著者 Lu, Zhichao| Furumatsu, Takayuki| Fujii, Masataka| Maehara, Ami| Ozaki, Toshifumi|
抄録 BACKGROUND: The meniscus plays an important role in controlling the complex biomechanics of the knee. Meniscus injury is common in the knee joint. The perimeniscal capillary plexus supplies the outer meniscus, whereas the inner meniscus is composed of avascular tissue. Angiogenesis factors, such as vascular endothelial growth factor (VEGF), have important roles in promoting vascularization of various tissues. VEGF-mediated neovascularization is beneficial to the healing of injured tissues. However, the distribution and angiogenic role of VEGF remains unclear in the meniscus and injured meniscus. We hypothesized that VEGF could affect meniscus cells and modulate the meniscus healing process. METHODS: Menisci were obtained from total knee arthroplasty patients. Meniscal injury was created ex vivo by a microsurgical blade. VEGF mRNA and protein expression were detected by the polymerase chain reaction and immunohistochemical analyses, respectively. RESULTS: In native meniscal tissue, the expression of VEGF and HIF-1α mRNAs could not be detected. However, VEGF and HIF-1α mRNAs were found in cultured meniscal cells (VEGF: outer > inner; HIF-1α: outer = inner). Injury increased mRNA levels of both VEGF and HIF-1α, with the increase being greatest in the outer area. Immunohistochemical analyses revealed that VEGF protein was detected mainly in the outer region and around injured areas of the meniscus. However, VEGF concentrations were similar between inner and outer menisci-derived media. CONCLUSIONS: This study demonstrated that both the inner and outer regions of the meniscus contained VEGF. HIF-1α expression and VEGF deposition were high in injured meniscal tissue. Our results suggest that injury stimulates the expression of HIF-1α and VEGF that may be preserved in the extracellular matrix as the healing stimulator of damaged meniscus, especially in the outer meniscus.
キーワード vascular endothelial growth factor (VEGF) meniscus meniscal injury hypoxia-inducible factor-1α (HIF-1α)
備考 This is an Accepted Manuscript of an article published by Elsevier This fulltext availavle in Jul 2018
発行日 2017-07
出版物タイトル Journal of Orthopaedic Science
22巻
4号
出版者 Elsevier
開始ページ 715
終了ページ 721
ISSN 0949-2658
NCID AA11052566
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
PubMed ID 28318650
DOI 10.1016/j.jos.2017.02.006
Web of Sience KeyUT 000407394700022
関連URL isVersionOf https://doi.org/10.1016/j.jos.2017.02.006
著者 Furumatsu, Takayuki| Fujii, Masataka| Kodama, Yuya| Ozaki, Toshifumi|
抄録 BACKGROUND: The posterior root ligament of the medial meniscus (MM) has a critical role in regulating the MM movement. An accurate diagnosis of the MM posterior root tear (MMPRT) using magnetic resonance imaging (MRI) is important for preventing sequential osteoarthritis following the MMPRT. However, diagnosis of the MMPRT is relatively difficult even after using several characteristic MRI findings. The aim of this study was to identify a useful meniscal body sign of the MMPRT for improving diagnostic MRI reading. METHODS: Eighty-five patients who underwent surgical treatments for the MMPRT (39 knees) and other types of MM tears (49 knees) were included. The presence of characteristic MRI findings such as cleft sign, ghost sign, radial tear sign, medial extrusion sign, and new meniscal body shape-oriented "giraffe neck sign" was evaluated in 120 MRI examinations. RESULTS: Giraffe neck signs were observed in 81.7% of the MMPRTs and in 3.3% of other MM tears. Cleft, ghost, and radial tear signs were highly positive in the MMPRTs compared with other MM tears. Medial extrusion signs were frequently observed in both groups. Coexistence rates of any 2 MRI signs, except for medial extrusion sign, were 91.7% in the MMPRT group and 5% in other MM tears. CONCLUSIONS: This study demonstrated that a new characteristic MRI finding "giraffe neck sign" was observed in 81.7% of the MMPRT. Our results suggest that the combination of giraffe neck, cleft, ghost, and radial tear signs may be important for an accurate diagnostic MRI reading of the MMPRT.
備考 This is an Accepted Manuscript of an article published by Elsevier This fulltext availavle in Jul 2018
発行日 2017-07
出版物タイトル Journal of Orthopaedic Science
22巻
4号
出版者 Elsevier
開始ページ 731
終了ページ 736
ISSN 0949-2658
NCID AA11052566
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
PubMed ID 28416188
DOI 10.1016/j.jos.2017.03.013
Web of Sience KeyUT 000407394700025
関連URL isVersionOf https://doi.org/10.1016/j.jos.2017.03.013
著者 Ghosh, Souvik| Gatheru, Zipporah| Nyangao, James| Adachi, Noriaki| Urushibara, Noriko| Kobayashi, Nobumichi|
発行日 2011-01
出版物タイトル Infection, Genetics and Evolution
11巻
1号
資料タイプ 学術雑誌論文
著者 Yan, He| Li, Lin| Alam, M. Jahangir| Shinoda, Sumio| Miyoshi, Shin-ichi| Shi, Lei|
発行日 2010-10-15
出版物タイトル International Journal of Food Microbiology
143巻
3号
資料タイプ 学術雑誌論文
著者 Wajima, Takeaki| Sabui, Subrata| Fukumoto, Megumi| Kano, Shigeyuki| Ramamurthy, Thandavarayan| Chatterjee, Nabendu Sekhar| Hamabata, Takashi|
発行日 2011-10
出版物タイトル Microbial Pathogenesis
51巻
4号
資料タイプ 学術雑誌論文
著者 Miyoshi, Shin-ichi| Abe, Yuki| Senoh, Mitsutoshi| Mizuno, Tamaki| Maehara, Yoko| Nakao, Hiroshi|
発行日 2011-05
出版物タイトル Toxicon
57巻
6号
資料タイプ 学術雑誌論文
著者 Khan, Shahbaz Manzoor| Debnath, Chanchal| Pramanik, Amiya Kumar| Xiao, Lihua| Nozaki, Tomoyoshi| Ganguly, Sandipan|
発行日 2011-06-10
出版物タイトル Veterinary Parasitology
178巻
3-4号
資料タイプ 学術雑誌論文
著者 Khan, Shahbaz Manzoor| Debnath, Chanchal| Pramanik, Amiya Kumar| Xiao, Lihua| Nozaki, Tomoyoshi| Ganguly, Sandipan|
発行日 2010-07-15
出版物タイトル Veterinary Parasitology
171巻
1-2号
資料タイプ 学術雑誌論文
著者 Muraoka, Y| Saeki, K| Yao, Y| Wakita, T| Hirai, M| Yokoya, T| Eguchi, R| Shin, S|
発行日 2010-08
出版物タイトル Journal of Electron Spectroscopy and Related Phenomena
181巻
2-3号
資料タイプ 学術雑誌論文
著者 Wakita, Takanori| Okazaki, Hiroyuki| Takano, Yoshihiko| Hirai, Masaaki| Muraoka, Yuji| Yokoya, Takayoshi|
発行日 2010-12
出版物タイトル Physica C-Superconductivity and its Applications
470巻
S1号
資料タイプ 学術雑誌論文
著者 Yoshida, Rikiya| Wakita, Takanori| Okazaki, Hiroyuki| Mizuguchi, Yoshikazu| Tsuda, Shunsuke| Takano, Yoshihiko| Takeya, Hiroyuki| Hirata, Kazuto| Kato, Yukako| Muro, Takayuki| Okawa, Mario| Ishizaka, Kyoko| Shin, Shik| Harima, Hisatomo| Hirai, Masaaki| Muraoka, Yuji| Yokoya, Takayoshi|
発行日 2010-12
出版物タイトル Physica C-Superconductivity and its Applications
470巻
S1号
資料タイプ 学術雑誌論文
著者 Okazaki, Hiroyuki| Yoshida, Rikiya| Iwai, Keisuke| Noami, Kengo| Muro, Takayuki| Nakamura, Tetsuya| Wakita, Takanori| Muraoka, Yuji| Hirai, Masaaki| Tomioka, Fumiaki| Takano, Yoshihiko| Takenaka, Asami| Toyoda, Masahiro| Oguch, Tamio| Yokoya, Takayoshi|
発行日 2010-12
出版物タイトル Physica C-Superconductivity and its Applications
470巻
S1号
資料タイプ 学術雑誌論文
著者 Nishioka, Yasutaka| Suzumori, Koichi| Kanda, Takefumi| Wakimoto, Shuichi|
発行日 2010-12
出版物タイトル Sensors and Actuators A: Physical
164巻
1-2号
資料タイプ 学術雑誌論文
著者 Nohara, Minoru| Kakiya, Satomi| Kudo, Kazutaka| Oshiro, Yoshihiro| Araki, Shingo| Kobayashi, Tatsuo C.| Oku, Kenta| Nishibori, Eiji| Sawa, Hiroshi|
発行日 2012-04
出版物タイトル Solid State Communications
152巻
8号
資料タイプ 学術雑誌論文
著者 Matsuura, Koji| Hayashi, Nobuyoshi| Kuroda, Yuka| Takiue, Chisato| Hirata, Rei| Takenami, Mami| Aoi, Yoko| Yoshioka, Nanako| Habara, Toshihiro| Mukaida, Tetsunori| Naruse, Keiji|
発行日 2010-03
出版物タイトル Reproductive BioMedicine Online
20巻
3号
資料タイプ 学術雑誌論文
著者 Sano, Hikaru| Matsuura, Koji| Naruse, Keiji| Funahashi, Hiroaki|
発行日 2010-09-15
出版物タイトル Theriogenology
74巻
5号
資料タイプ 学術雑誌論文
著者 Matsuura, Koji| Hayashi, Nobuyoshi| Takiue, Chisato| Hirata, Rei| Habara, Toshihiro| Naruse, Keiji|
発行日 2010-08
出版物タイトル Fertility and Sterility
94巻
3号
資料タイプ 学術雑誌論文
著者 Matsuura, Koji| Takenami, Mami| Kuroda, Yuka| Hyakutake, Toru| Yanase, Shinichiro| Naruse, Keiji|
発行日 2012-01
出版物タイトル Reproductive BioMedicine Online
24巻
1号
資料タイプ 学術雑誌論文
著者 Yoshioka-Nishimura, Miho| Yamamoto, Yasusi|
発行日 2014
出版物タイトル Journal of Photochemistry and Photobiology B: Biology
137巻
資料タイプ 学術雑誌論文