| フルテキストURL | J_Orthop_Sci_21_4_524.pdf fig.pdf |
|---|---|
| 著者 | Furumatsu, Takayuki| Maehara, Ami| Ozaki, Toshifumi| |
| 抄録 | BACKGROUND: Proper functioning of the meniscus depends on the composition and organization of its fibrocartilaginous extracellular matrix. We previously demonstrated that the avascular inner meniscus has a more chondrocytic phenotype compared with the outer meniscus. Inhibition of the Rho family GTPase ROCK, the major regulator of the actin cytoskeleton, stimulates the chondrogenic transcription factor Sry-type HMG box (SOX) 9-dependent α1(II) collagen (COL2A1) expression in inner meniscus cells. However, the crosstalk between ROCK inhibition, SOX9, and other transcription modulators on COL2A1 upregulation remains unclear in meniscus cells. The aim of this study was to investigate the role of SOX9-related transcriptional complex on COL2A1 expression under the inhibition of ROCK in human meniscus cells. METHODS: Human inner and outer meniscus cells were prepared from macroscopically intact lateral menisci. Cells were cultured in the presence or absence of ROCK inhibitor (ROCKi, Y27632). Gene expression, collagen synthesis, and nuclear translocation of SOX9 and Smad2/3 were analyzed. RESULTS: Treatment of ROCKi increased the ratio of type I/II collagen double positive cells derived from the inner meniscus. In real-time PCR analyses, expression of SOX9 and COL2A1 genes was stimulated by ROCKi treatment in inner meniscus cells. ROCKi treatment also induced nuclear translocation of SOX9 and phosphorylated Smad2/3 in immunohistological analyses. Complex formation between SOX9 and Smad3 was increased by ROCKi treatment in inner meniscus cells. Chromatin immunoprecipitation analyses revealed that association between SOX9/Smad3 transcriptional complex with the COL2A1 enhancer region was increased by ROCKi treatment. CONCLUSIONS: This study demonstrated that ROCK inhibition stimulated SOX9/Smad3-dependent COL2A1 expression through the immediate nuclear translocation of Smad3 in inner meniscus cells. Our results suggest that ROCK inhibition can stimulates type II collagen synthesis through the cooperative activation of Smad3 in inner meniscus cells. ROCKi treatment may be useful to promote the fibrochondrocytic healing of the injured inner meniscus. |
| 備考 | This is an Accepted Manuscript of an article published by Elsevier |
| 発行日 | 2016-07 |
| 出版物タイトル | Journal of Orthopaedic Science |
| 巻 | 21巻 |
| 号 | 4号 |
| 出版者 | Elsevier |
| 開始ページ | 524 |
| 終了ページ | 529 |
| ISSN | 0949-2658 |
| NCID | AA11052566 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | English |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja |
| 論文のバージョン | author |
| PubMed ID | 27113646 |
| DOI | 10.1016/j.jos.2016.02.013 |
| Web of Sience KeyUT | 000381142800020 |
| 関連URL | isVersionOf https://doi.org/10.1016/j.jos.2016.02.013 |
| 著者 | Lu, Zhichao| Furumatsu, Takayuki| Fujii, Masataka| Maehara, Ami| Ozaki, Toshifumi| |
|---|---|
| 抄録 | BACKGROUND: The meniscus plays an important role in controlling the complex biomechanics of the knee. Meniscus injury is common in the knee joint. The perimeniscal capillary plexus supplies the outer meniscus, whereas the inner meniscus is composed of avascular tissue. Angiogenesis factors, such as vascular endothelial growth factor (VEGF), have important roles in promoting vascularization of various tissues. VEGF-mediated neovascularization is beneficial to the healing of injured tissues. However, the distribution and angiogenic role of VEGF remains unclear in the meniscus and injured meniscus. We hypothesized that VEGF could affect meniscus cells and modulate the meniscus healing process. METHODS: Menisci were obtained from total knee arthroplasty patients. Meniscal injury was created ex vivo by a microsurgical blade. VEGF mRNA and protein expression were detected by the polymerase chain reaction and immunohistochemical analyses, respectively. RESULTS: In native meniscal tissue, the expression of VEGF and HIF-1α mRNAs could not be detected. However, VEGF and HIF-1α mRNAs were found in cultured meniscal cells (VEGF: outer > inner; HIF-1α: outer = inner). Injury increased mRNA levels of both VEGF and HIF-1α, with the increase being greatest in the outer area. Immunohistochemical analyses revealed that VEGF protein was detected mainly in the outer region and around injured areas of the meniscus. However, VEGF concentrations were similar between inner and outer menisci-derived media. CONCLUSIONS: This study demonstrated that both the inner and outer regions of the meniscus contained VEGF. HIF-1α expression and VEGF deposition were high in injured meniscal tissue. Our results suggest that injury stimulates the expression of HIF-1α and VEGF that may be preserved in the extracellular matrix as the healing stimulator of damaged meniscus, especially in the outer meniscus. |
| キーワード | vascular endothelial growth factor (VEGF) meniscus meniscal injury hypoxia-inducible factor-1α (HIF-1α) |
| 備考 | This is an Accepted Manuscript of an article published by Elsevier This fulltext availavle in Jul 2018 |
| 発行日 | 2017-07 |
| 出版物タイトル | Journal of Orthopaedic Science |
| 巻 | 22巻 |
| 号 | 4号 |
| 出版者 | Elsevier |
| 開始ページ | 715 |
| 終了ページ | 721 |
| ISSN | 0949-2658 |
| NCID | AA11052566 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | English |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja |
| 論文のバージョン | author |
| PubMed ID | 28318650 |
| DOI | 10.1016/j.jos.2017.02.006 |
| Web of Sience KeyUT | 000407394700022 |
| 関連URL | isVersionOf https://doi.org/10.1016/j.jos.2017.02.006 |
| 著者 | Furumatsu, Takayuki| Fujii, Masataka| Kodama, Yuya| Ozaki, Toshifumi| |
|---|---|
| 抄録 | BACKGROUND: The posterior root ligament of the medial meniscus (MM) has a critical role in regulating the MM movement. An accurate diagnosis of the MM posterior root tear (MMPRT) using magnetic resonance imaging (MRI) is important for preventing sequential osteoarthritis following the MMPRT. However, diagnosis of the MMPRT is relatively difficult even after using several characteristic MRI findings. The aim of this study was to identify a useful meniscal body sign of the MMPRT for improving diagnostic MRI reading. METHODS: Eighty-five patients who underwent surgical treatments for the MMPRT (39 knees) and other types of MM tears (49 knees) were included. The presence of characteristic MRI findings such as cleft sign, ghost sign, radial tear sign, medial extrusion sign, and new meniscal body shape-oriented "giraffe neck sign" was evaluated in 120 MRI examinations. RESULTS: Giraffe neck signs were observed in 81.7% of the MMPRTs and in 3.3% of other MM tears. Cleft, ghost, and radial tear signs were highly positive in the MMPRTs compared with other MM tears. Medial extrusion signs were frequently observed in both groups. Coexistence rates of any 2 MRI signs, except for medial extrusion sign, were 91.7% in the MMPRT group and 5% in other MM tears. CONCLUSIONS: This study demonstrated that a new characteristic MRI finding "giraffe neck sign" was observed in 81.7% of the MMPRT. Our results suggest that the combination of giraffe neck, cleft, ghost, and radial tear signs may be important for an accurate diagnostic MRI reading of the MMPRT. |
| 備考 | This is an Accepted Manuscript of an article published by Elsevier This fulltext availavle in Jul 2018 |
| 発行日 | 2017-07 |
| 出版物タイトル | Journal of Orthopaedic Science |
| 巻 | 22巻 |
| 号 | 4号 |
| 出版者 | Elsevier |
| 開始ページ | 731 |
| 終了ページ | 736 |
| ISSN | 0949-2658 |
| NCID | AA11052566 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | English |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja |
| 論文のバージョン | author |
| PubMed ID | 28416188 |
| DOI | 10.1016/j.jos.2017.03.013 |
| Web of Sience KeyUT | 000407394700025 |
| 関連URL | isVersionOf https://doi.org/10.1016/j.jos.2017.03.013 |
| 著者 | Ghosh, Souvik| Gatheru, Zipporah| Nyangao, James| Adachi, Noriaki| Urushibara, Noriko| Kobayashi, Nobumichi| |
|---|---|
| 発行日 | 2011-01 |
| 出版物タイトル | Infection, Genetics and Evolution |
| 巻 | 11巻 |
| 号 | 1号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | Yan, He| Li, Lin| Alam, M. Jahangir| Shinoda, Sumio| Miyoshi, Shin-ichi| Shi, Lei| |
|---|---|
| 発行日 | 2010-10-15 |
| 出版物タイトル | International Journal of Food Microbiology |
| 巻 | 143巻 |
| 号 | 3号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | Wajima, Takeaki| Sabui, Subrata| Fukumoto, Megumi| Kano, Shigeyuki| Ramamurthy, Thandavarayan| Chatterjee, Nabendu Sekhar| Hamabata, Takashi| |
|---|---|
| 発行日 | 2011-10 |
| 出版物タイトル | Microbial Pathogenesis |
| 巻 | 51巻 |
| 号 | 4号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | Miyoshi, Shin-ichi| Abe, Yuki| Senoh, Mitsutoshi| Mizuno, Tamaki| Maehara, Yoko| Nakao, Hiroshi| |
|---|---|
| 発行日 | 2011-05 |
| 出版物タイトル | Toxicon |
| 巻 | 57巻 |
| 号 | 6号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | Khan, Shahbaz Manzoor| Debnath, Chanchal| Pramanik, Amiya Kumar| Xiao, Lihua| Nozaki, Tomoyoshi| Ganguly, Sandipan| |
|---|---|
| 発行日 | 2011-06-10 |
| 出版物タイトル | Veterinary Parasitology |
| 巻 | 178巻 |
| 号 | 3-4号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | Khan, Shahbaz Manzoor| Debnath, Chanchal| Pramanik, Amiya Kumar| Xiao, Lihua| Nozaki, Tomoyoshi| Ganguly, Sandipan| |
|---|---|
| 発行日 | 2010-07-15 |
| 出版物タイトル | Veterinary Parasitology |
| 巻 | 171巻 |
| 号 | 1-2号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | Muraoka, Y| Saeki, K| Yao, Y| Wakita, T| Hirai, M| Yokoya, T| Eguchi, R| Shin, S| |
|---|---|
| 発行日 | 2010-08 |
| 出版物タイトル | Journal of Electron Spectroscopy and Related Phenomena |
| 巻 | 181巻 |
| 号 | 2-3号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | Wakita, Takanori| Okazaki, Hiroyuki| Takano, Yoshihiko| Hirai, Masaaki| Muraoka, Yuji| Yokoya, Takayoshi| |
|---|---|
| 発行日 | 2010-12 |
| 出版物タイトル | Physica C-Superconductivity and its Applications |
| 巻 | 470巻 |
| 号 | S1号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | Yoshida, Rikiya| Wakita, Takanori| Okazaki, Hiroyuki| Mizuguchi, Yoshikazu| Tsuda, Shunsuke| Takano, Yoshihiko| Takeya, Hiroyuki| Hirata, Kazuto| Kato, Yukako| Muro, Takayuki| Okawa, Mario| Ishizaka, Kyoko| Shin, Shik| Harima, Hisatomo| Hirai, Masaaki| Muraoka, Yuji| Yokoya, Takayoshi| |
|---|---|
| 発行日 | 2010-12 |
| 出版物タイトル | Physica C-Superconductivity and its Applications |
| 巻 | 470巻 |
| 号 | S1号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | Okazaki, Hiroyuki| Yoshida, Rikiya| Iwai, Keisuke| Noami, Kengo| Muro, Takayuki| Nakamura, Tetsuya| Wakita, Takanori| Muraoka, Yuji| Hirai, Masaaki| Tomioka, Fumiaki| Takano, Yoshihiko| Takenaka, Asami| Toyoda, Masahiro| Oguch, Tamio| Yokoya, Takayoshi| |
|---|---|
| 発行日 | 2010-12 |
| 出版物タイトル | Physica C-Superconductivity and its Applications |
| 巻 | 470巻 |
| 号 | S1号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | Nishioka, Yasutaka| Suzumori, Koichi| Kanda, Takefumi| Wakimoto, Shuichi| |
|---|---|
| 発行日 | 2010-12 |
| 出版物タイトル | Sensors and Actuators A: Physical |
| 巻 | 164巻 |
| 号 | 1-2号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | Nohara, Minoru| Kakiya, Satomi| Kudo, Kazutaka| Oshiro, Yoshihiro| Araki, Shingo| Kobayashi, Tatsuo C.| Oku, Kenta| Nishibori, Eiji| Sawa, Hiroshi| |
|---|---|
| 発行日 | 2012-04 |
| 出版物タイトル | Solid State Communications |
| 巻 | 152巻 |
| 号 | 8号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | Matsuura, Koji| Hayashi, Nobuyoshi| Kuroda, Yuka| Takiue, Chisato| Hirata, Rei| Takenami, Mami| Aoi, Yoko| Yoshioka, Nanako| Habara, Toshihiro| Mukaida, Tetsunori| Naruse, Keiji| |
|---|---|
| 発行日 | 2010-03 |
| 出版物タイトル | Reproductive BioMedicine Online |
| 巻 | 20巻 |
| 号 | 3号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | Sano, Hikaru| Matsuura, Koji| Naruse, Keiji| Funahashi, Hiroaki| |
|---|---|
| 発行日 | 2010-09-15 |
| 出版物タイトル | Theriogenology |
| 巻 | 74巻 |
| 号 | 5号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | Matsuura, Koji| Hayashi, Nobuyoshi| Takiue, Chisato| Hirata, Rei| Habara, Toshihiro| Naruse, Keiji| |
|---|---|
| 発行日 | 2010-08 |
| 出版物タイトル | Fertility and Sterility |
| 巻 | 94巻 |
| 号 | 3号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | Matsuura, Koji| Takenami, Mami| Kuroda, Yuka| Hyakutake, Toru| Yanase, Shinichiro| Naruse, Keiji| |
|---|---|
| 発行日 | 2012-01 |
| 出版物タイトル | Reproductive BioMedicine Online |
| 巻 | 24巻 |
| 号 | 1号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | Yoshioka-Nishimura, Miho| Yamamoto, Yasusi| |
|---|---|
| 発行日 | 2014 |
| 出版物タイトル | Journal of Photochemistry and Photobiology B: Biology |
| 巻 | 137巻 |
| 資料タイプ | 学術雑誌論文 |