このエントリーをはてなブックマークに追加
ID 52742
フルテキストURL
著者
Takahara, Masahiro Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch
Nemoto, Yasuhiro Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch
Oshima, Shigeru Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch
Matsuzawa, Yu Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch
Kanai, Takanori Keio Univ, Dept Internal Med, Div Gastroenterol & Hepatol, Sch Med
Okamoto, Ryuichi Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch
Tsuchiya, Kiichiro Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch
Nakamura, Tetsuya Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch
Yamamoto, Kazuhide Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Gastroenterol & Hepatol
Watanabe, Mamoru Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch
抄録
Colitogenic memory CD4(+) T cells are important in the pathogenesis of inflammatory bowel disease (IBD). Although memory stem cells with high survival and self-renewal capacity were recently identified in both mice and humans, it is unclear whether a similar subset is present in chronic colitis mice. We sought to identify and purify a long-lived subset of colitogenic memory CD4(+) T cells, which may be targets for treatment of IBD. A long-lived subset of colitogenic memory CD4(+) T cells was purified using a long-term culture system. The characteristics of these cells were assessed. Interleukin (IL)-7 promoted the in vitro survival for >8 weeks of lamina propria (LP) CD4(+) T cells from colitic SOD mice previously injected with CD4(+)CD45RB(high) T cells. These cells were in a quiescent state and divided a maximum of 5 times in 4 weeks. LP CD4(+) T cells expressed higher levels of Bcl-2, integrin-alpha 4 beta 7, CXCR3 and CD25 after than before culture, as well as secreting high concentrations of IL-2 and low concentrations of IFN-gamma and IL-17 in response to intestinal bacterial antigens. LP CD4(+) T cells from colitic mice cultured with IL-7 for 8 weeks induced more severe colitis than LP CD4(+) T cells cultured for 4 weeks. We developed a novel culture system to purify a long-lived, highly pathogenic memory subset from activated LP CD4(+) T cells. IL-7 promoted long-term in vitro survival of this subset in a quiescent state. This subset will be a novel, effective target for the treatment of IBD.
キーワード
Inflammatory bowel disease
Memory T cells
CD4
IL-7
Cell culture
発行日
2013-12
出版物タイトル
Immunology Letters
156巻
1-2号
出版者
Elsevier Science BV.
開始ページ
82
終了ページ
93
ISSN
0165-2478
NCID
AA00231960
資料タイプ
学術雑誌論文
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/52510
言語
English
OAI-PMH Set
岡山大学
著作権者
© 2013 Elsevier B.V. All rights reserved.
論文のバージョン
author
査読
有り
DOI
Web of Sience KeyUT