start-ver=1.4 cd-journal=joma no-vol=961 cd-vols= no-issue=2 article-no= start-page=179 end-page=189 dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=20030131 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A Periaxonal Net in the Zebrafish Central Nervous System en-subtitle= kn-subtitle= en-abstract= kn-abstract=We produced a monoclonal antibody, named A20, which specifically recognizes a 35 kDa protein and stains myelinated axons in zebrafish brain. The A20 antigen is located at the outside of the myelin layer of large axons, and comprises a fine meshwork composed of thin unit fibers about 1?2 μm in length and about 100?200 nm in thickness. The unit fibers form pentagonal and hexagonal structures, which further polymerize into an envelope structure on the axons. The A20 monoclonal antibody did not stain neuronal cell bodies nor synapses. Instead, the distribution of the A20 antigen was along axons, practically coincident with the distribution of myelin basic protein. The monoclonal antibody stained only axons in the central nervous system (CNS), and not the extracellular matrix surrounding Schwann cells. These results suggest that this antigenic meshwork (which we call the periaxonal net) is synthesized by oligodendrocytes. During the development of the zebrafish brain, the periaxonal net appeared after the formation of myelin on the axons. The periaxonal net developed first at the brain stem, then gradually appeared at the caudal end of the spinal cord. The thickness of the periaxonal net around the Mauthner axon changed during development. Although the thickness of the Mauthner axon continues to grow throughout life, the thickness of periaxonal net stopped growing at 6 months after fertilization. en-copyright= kn-copyright= en-aut-name=NakayasuHiroshi en-aut-sei=Nakayasu en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ArataNoriko en-aut-sei=Arata en-aut-mei=Noriko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Department of Biology, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Biology, Faculty of Science, Okayama University en-keyword=Oligodendrocyte kn-keyword=Oligodendrocyte en-keyword=Monoclonal antibody kn-keyword=Monoclonal antibody en-keyword=Extracellular matrix kn-keyword=Extracellular matrix en-keyword=Central nervous system kn-keyword=Central nervous system END start-ver=1.4 cd-journal=joma no-vol=481 cd-vols= no-issue=2 article-no= start-page=311 end-page=319 dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=20030403 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Ultratrace determination of phosphorus in ultrapurified water by a slope comparison method en-subtitle= kn-subtitle= en-abstract= kn-abstract=The analytical method for the determination of phosphorus in ultrapurified water was developed. Ultrapurified water was evaporated to concentrate phosphorus and the final sample volume for analysis was 10 ml. In 0.55 mol 1(-1) HCl, orthophosphate forms molybdophosphate, and then the molybdophosphate forms ion associate with Malachite Green (MG), which can be collected on a tiny membrane filter (diameter: 5 mm, and effective filtering diameter: 2 mm). After the ion associate on the membrane filter is dissolved together with the membrane filter in I ml of methyl cellosolve (MC), the absorbance of MC solution is measured at 627 nm by a flow injection-spectrophotometric detection technique. When 10 ml of the sample solution was used for the procedures and absorbance measurement, the calibration graph is linear up to about 500 ng 1(-1) of phosphorus and the detection limit was 8 ng 1(-1) (S/N = 3). For the determination of phosphorus in an ultrapurified water, 10-40 ml of sample solutions were transferred into poly(tetrafluoroethylene) (PTFE) beaker and evaporated to 5 ml or to dryness. To them, 0.003 mol 1(-1) HCl was added to get 10 ml of final solution, which was used as sample. Phosphate is determined by comparing the slope of the varied sample volume after evaporation/concentration with a slope of the standard calibration graph (a slope comparison method: SCM). The SCM enables to evaluate the concentration of phosphate in ultrapurified waters more sensitively and accurately. en-copyright= kn-copyright= en-aut-name=SabarudinAkhmad en-aut-sei=Sabarudin en-aut-mei=Akhmad kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OshimaMitsuko en-aut-sei=Oshima en-aut-mei=Mitsuko kn-aut-name=大島光子 kn-aut-sei=大島 kn-aut-mei=光子 aut-affil-num=2 ORCID= en-aut-name=MotomizuShoji en-aut-sei=Motomizu en-aut-mei=Shoji kn-aut-name=本水昌二 kn-aut-sei=本水 kn-aut-mei=昌二 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University en-keyword=ultrapurified water kn-keyword=ultrapurified water en-keyword=evaporation/concentration kn-keyword=evaporation/concentration en-keyword=ion associate kn-keyword=ion associate en-keyword=molybdophosphate kn-keyword=molybdophosphate en-keyword=malachite green kn-keyword=malachite green en-keyword=slope comparison method kn-keyword=slope comparison method END start-ver=1.4 cd-journal=joma no-vol=532 cd-vols= no-issue=1 article-no= start-page=27 end-page=35 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050507 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Slope comparison method (SCM) for the determination of trace amounts of silicate in ultrapurified water en-subtitle= kn-subtitle= en-abstract= kn-abstract=A sensitive analytical method for the determination of trace amounts of silicate in ultrapurified water was developed. The method is based on the formation of an ion associate of molybdosilicate with malachite green (MG) and the collection of the ion associate on a tiny membrane filter (diameter: 5 mm, and effective filtering diameter: 1 mm). The ion associate formed on the membrane filter is dissolved together with the membrane filter in 1 ml of methyl cellosolve (MC) and the absorbance of MC solution is measured at 627 nm by a flow injection-spectrophotometric detection technique. In this method, silicate in the original sample (ultrapurified water) is concentrated as the ion associate into a small volume of MC to get high sensitivity. As sample concentration takes place, the small amounts of silicate contained in the reagents used also become concentrated as the ion associate into MC. The original sample volumes are varied and evaporated to an identical volume. Therefore, the reagent added is fixed to the same volume. The absorbance increase linearly with increase in the original sample volume will be due only to silicate in the original samples (ultrapurified water). The resulting slopes obtained by varying the sample volumes are compared with the slope of the calibration graph, and thus named the slope comparison method (SCM). The SCM facilitates a more sensitive and accurate evaluation of silicate concentration in the samples than either common calibration method (CCM) or standard addition method (SAM) because it compensates for the influence of trace amounts of silicate contained in chemicals, reagent solution and solvent used. The calibration graph was constructed from 0 to 0.25 ng ml(-1) of Si and the detection limit was 10 pg ml(-1) (ppt) when 30 ml of samples was used. The standard deviation and relative standard deviation from six measurements of the reagent blanks were 0.0012 and 3.5%, respectively. en-copyright= kn-copyright= en-aut-name=SabarudinAkhmad en-aut-sei=Sabarudin en-aut-mei=Akhmad kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OshimaMitsuko en-aut-sei=Oshima en-aut-mei=Mitsuko kn-aut-name=大島光子 kn-aut-sei=大島 kn-aut-mei=光子 aut-affil-num=2 ORCID= en-aut-name=MotomizuShoji en-aut-sei=Motomizu en-aut-mei=Shoji kn-aut-name=本水昌二 kn-aut-sei=本水 kn-aut-mei=昌二 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University en-keyword=slope comparison method kn-keyword=slope comparison method en-keyword=ultrapurified water kn-keyword=ultrapurified water en-keyword=ion associate kn-keyword=ion associate en-keyword=molybdosilicate kn-keyword=molybdosilicate en-keyword=malachite green kn-keyword=malachite green en-keyword=membrane filter kn-keyword=membrane filter END start-ver=1.4 cd-journal=joma no-vol=542 cd-vols= no-issue=2 article-no= start-page=207 end-page=215 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050629 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Synthesis of chitosan resin possessing 3,4-diamino benzoic acid moiety for the collection/concentration of arsenic and selenium in water samples and their measurement by inductively coupled plasma-mass spectrometry en-subtitle= kn-subtitle= en-abstract= kn-abstract=A chitosan resin functionalized with 3,4-diamino benzoic acid (CCTS-DBA resin) was newly synthesized by using a cross-linked chitosan (CCTS) as base material. The adsorption behavior of trace amounts of elements on the CCTS-DBA resin was examined by the pretreatment with a mini-column and measurement of the elements by inductively coupled plasma-Mass spectrometry (ICP-MS). Arsenic(V) could be retained on the CCTS-DBA resin at pH 3 as an oxoanion of H(2)ASO(4)(-). Selenium(VI) is strongly adsorbed at pH 2 and pH 3 as an oxoanion of SeO42-. while selenium(IV) as HSeO3- is adsorbed on the resin at pH 3. The sorption capacities are 82, 64, and 88 mg g(-1) resin for As(V), Se(IV), and Se(VI), respectively. The effect of common anions and cations on the adsorption of As(V), Se(IV), and Se(VI) were studied; there was no interference from such anionic matrices as chloride, sulfate, phosphate, and nitrate up to 20 ppm, as well as from such artificial river water matrices as Na, K, Mg, and Ca after passing samples through the mini-column containing the resin. The CCTS-DBA resin was applied to the collection of arsenic and selenium species in bottled drinking water, tap water, and river water. en-copyright= kn-copyright= en-aut-name=SabarudinAkhmad en-aut-sei=Sabarudin en-aut-mei=Akhmad kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OshitaKoji en-aut-sei=Oshita en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OshimaMitsuko en-aut-sei=Oshima en-aut-mei=Mitsuko kn-aut-name=大島光子 kn-aut-sei=大島 kn-aut-mei=光子 aut-affil-num=3 ORCID= en-aut-name=MotomizuShoji en-aut-sei=Motomizu en-aut-mei=Shoji kn-aut-name=本水昌二 kn-aut-sei=本水 kn-aut-mei=昌二 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=4 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University en-keyword=Cross-linked chitosan kn-keyword=Cross-linked chitosan en-keyword=3,4-diamino benzoic acid kn-keyword=3,4-diamino benzoic acid en-keyword=Arsenic kn-keyword=Arsenic en-keyword=Selenium kn-keyword=Selenium en-keyword=ICP-MS kn-keyword=ICP-MS END start-ver=1.4 cd-journal=joma no-vol=409 cd-vols= no-issue=4-6 article-no= start-page=187 end-page=191 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Fabrication and characterization of field-effect transistor device with C2v isomer of Pr@C82 en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A field-effect transistor (FET) device was fabricated with thin films of C2v isomer of Pr@C82. This device apparently showed n-channel normally-on type FET properties, where non-zero current was observed at gate-source voltage of 0 VGS, of 0V. Normally off FET properties were observed by subtraction of the non-zero current from the drain current.Thus the normally on properties are ascribed to the high bulk current caused by the small energy gap ?0.3 eV. The field-effect mobility for this FET was 1.5 x 10-4 cm2 V-1 s-1 at 320 K, being comparable to those of other endohedral metallofullerene FET devices.

en-copyright= kn-copyright= en-aut-name=NaganoTakayuki en-aut-sei=Nagano en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KuwaharaEiji en-aut-sei=Kuwahara en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakayanagiToshio en-aut-sei=Takayanagi en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KubozonoYoshihiro en-aut-sei=Kubozono en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=FujiwaraAkihiko en-aut-sei=Fujiwara en-aut-mei=Akihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=4 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=5 en-affil= kn-affil=CREST, Japan Science and Technology Agency en-keyword=Field effect transistors kn-keyword=Field effect transistors END start-ver=1.4 cd-journal=joma no-vol=413 cd-vols= no-issue=4-6 article-no= start-page=379 end-page=383 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050926 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Fabrication of a logic gate circuit based on ambipolar field-effect transistors with thin films of C60 and pentacene en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Ambipolar field-effect transistor (FET) devices were fabricated with a heterostructure of C60 and pentacene, and their p- and n-channel field-effect mobilities were studied as a function of thickness of pentacene thin-films. The observed dependences of the μ values were interpreted in terms of the morphology of the thin films and the band structure of C60/pentacene heterostructure. A complementary metal-oxide-semiconductor (CMOS) circuit was fabricated by integration of two ambipolar FETs, aiming at realization of a new CMOS inverter circuit composed of FETs with the same device structure. The gain of 4, the threshold voltage of 85 V, and the complex output characteristics were explained on the basis of the properties of the component FET devices.

en-copyright= kn-copyright= en-aut-name=KuwaharaEiji en-aut-sei=Kuwahara en-aut-mei=Eiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KusaiHaruka en-aut-sei=Kusai en-aut-mei=Haruka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NaganoTakayuki en-aut-sei=Nagano en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TakayanagiToshio en-aut-sei=Takayanagi en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KubozonoYoshihiro en-aut-sei=Kubozono en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=4 en-affil= kn-affil=Department of Chemistry, Okayama University affil-num=5 en-affil= kn-affil=Department of Chemistry, Okayama University en-keyword=Band structure kn-keyword=Band structure en-keyword=Carbon kn-keyword=Carbon en-keyword=CMOS integrated circuits kn-keyword=CMOS integrated circuits en-keyword=Field effect transistors kn-keyword=Field effect transistors en-keyword=Logic gates kn-keyword=Logic gates en-keyword=Thin films kn-keyword=Thin films en-keyword=Threshold voltage kn-keyword=Threshold voltage en-keyword=Band structures kn-keyword=Band structures en-keyword=Logic gate circuits kn-keyword=Logic gate circuits en-keyword=N-channel field-effective mobilities kn-keyword=N-channel field-effective mobilities en-keyword=Pentacene kn-keyword=Pentacene en-keyword=Logic circuits kn-keyword=Logic circuits END start-ver=1.4 cd-journal=joma no-vol=558 cd-vols= no-issue=1-2 article-no= start-page=246 end-page=253 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20060203 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Synthesis of a chitosan-based chelating resin and its application to the selective concentration and ultratrace determination of silver in environmental water samples en-subtitle= kn-subtitle= en-abstract= kn-abstract=A novel chelating resin using chitosan as a base material, ethylenediamine-type chitosan, has been synthesized for the first time in the present study, and applied to the collection/concentration of ultratrace amounts of silver in environmental water samples. In the present study, ultratrace amounts of silver collected on the resin were eluted and determined by ICP-MS. The resin packed in a 1 mL mini column could adsorb silver selectively and quantitatively at a flow rate of 2 mL min(-1) in the wide pH range from 1 to 8, and silver adsorbed on the resin could be easily recovered by passing 1 M nitric acid as an eluent into the column. High adsorption capacity for silver at pH 5, 0.37 mmol mL(-1) of the resin, was achieved, and t(1/2) of the adsorption is less than 5 min. The effect of chloride on the collection of silver was examined by varying chloride concentrations from 10(-4) to 0.75 M; the results showed that the present resin can be used for the collection/concentration of ultratrace amounts of silver in natural waters, as well as seawater. To ensure the accuracy and the precision of the method, CASS-4 near shore seawater reference material from the NRCC has been analyzed. This is not a certified SRM for silver, but has been used for comparative silver analysis by several groups, who report very similar results to those that are reported here. The developed method using ethylenediamine-type chitosan resin gives 0.7 pg mL(-1) of the detection limit when 50-fold enrichment was used. The proposed method was successfully applied to the determination of silver in tap, river, and seawater samples. en-copyright= kn-copyright= en-aut-name=KatarinaRosi Ketrin en-aut-sei=Katarina en-aut-mei=Rosi Ketrin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakayanagiToshio en-aut-sei=Takayanagi en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OshimaMitsuko en-aut-sei=Oshima en-aut-mei=Mitsuko kn-aut-name=大島光子 kn-aut-sei=大島 kn-aut-mei=光子 aut-affil-num=3 ORCID= en-aut-name=MotomizuShoji en-aut-sei=Motomizu en-aut-mei=Shoji kn-aut-name=本水昌二 kn-aut-sei=本水 kn-aut-mei=昌二 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=4 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University en-keyword=Chelating resin kn-keyword=Chelating resin en-keyword=Chitosan kn-keyword=Chitosan en-keyword=Determination of silver kn-keyword=Determination of silver en-keyword=Environmental water samples kn-keyword=Environmental water samples en-keyword=ICP-MS kn-keyword=ICP-MS END start-ver=1.4 cd-journal=joma no-vol=579 cd-vols= no-issue=1 article-no= start-page=33 end-page=37 dt-received= dt-revised= dt-accepted= dt-pub-year=2006 dt-pub=20061002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A membraneless gas diffusion unit: Design and its application to determination of ethanol in liquors by spectrophotometric flow injection en-subtitle= kn-subtitle= en-abstract= kn-abstract=This work presents new design of a gas diffusion unit, called 'membraneless gas diffusion (MGD) unit', which, unlike a conventional gas diffusion (GD) unit, allows selective detection of volatile compounds to be made without the need of a hydrophobic membrane. A flow injection method was developed employing the MGD unit to determine ethanol in alcoholic drinks based on the reduction of dichromate by ethanol vapor. Results clearly demonstrated that the MGD unit was suitable for determination of ethanol in beer, wine and distilled liquors. Detection limit (3S/N) of MGD unit was lower than the GD unit (GD: 0.68%, v/v; MGD: 0.27%, v/v). The MGD design makes the system more sensitive as mass transfer is more efficient than that of GD and thus, MGD can perfectly replace membrane-based designs. en-copyright= kn-copyright= en-aut-name=ChoengchanN. en-aut-sei=Choengchan en-aut-mei=N. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MantimT. en-aut-sei=Mantim en-aut-mei=T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WilairatP. en-aut-sei=Wilairat en-aut-mei=P. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=DasguptaP. K. en-aut-sei=Dasgupta en-aut-mei=P. K. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MotomizuShoji en-aut-sei=Motomizu en-aut-mei=Shoji kn-aut-name=本水昌二 kn-aut-sei=本水 kn-aut-mei=昌二 aut-affil-num=5 ORCID= en-aut-name=NacaprichaD. en-aut-sei=Nacapricha en-aut-mei=D. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Flow Innovation-Research for Science and Technology Laboratories, Department of Chemistry, Faculty of Science, Mahidol University affil-num=2 en-affil= kn-affil=Flow Innovation-Research for Science and Technology Laboratories, Department of Chemistry, Faculty of Science, Mahidol University affil-num=3 en-affil= kn-affil=Flow Innovation-Research for Science and Technology Laboratories, Department of Chemistry, Faculty of Science, Mahidol University affil-num=4 en-affil= kn-affil=Department of Chemistry and Biochemistry, Texas Tech University affil-num=5 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=6 en-affil= kn-affil=Flow Innovation-Research for Science and Technology Laboratories, Department of Chemistry, Faculty of Science, Mahidol University en-keyword=membraneless gas diffusion kn-keyword=membraneless gas diffusion en-keyword=flow injection kn-keyword=flow injection en-keyword=ethanol determination kn-keyword=ethanol determination en-keyword=liquor kn-keyword=liquor en-keyword=spectrophotometry kn-keyword=spectrophotometry END start-ver=1.4 cd-journal=joma no-vol=581 cd-vols= no-issue=2 article-no= start-page=214 end-page=220 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=20070109 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Functionalization of chitosan with 3,4-dihydroxybenzoic acid for the adsorption/collection of uranium in water samples and its determination by inductively coupled plasma-mass spectrometry en-subtitle= kn-subtitle= en-abstract= kn-abstract=A chitosan resin derivatized with 3,4-dihydroxybenzoic acid moiety (CCTS-DHBA resin) was newly synthesized for the collection/concentration of trace uranium by using cross-linked chitosan (CCTS) as base material, and the adsorption behavior of uranium as well as 60 elements on the resin was examined by passing the sample solutions through a mini-column packed with the resin. After the elution of the collected elements on the resin with 1M HNO3, the eluates were measured by inductively coupled plasma-mass spectrometry (ICP-MS). The CCTS-DHBA resin can adsorb several metal cations and several oxoanionic elements at appropriate pH. Among these metal ions, uranium shows an excellent adsorption behavior on this resin. Uranium as UO22+ species can be adsorbed on the resin by chelating mechanism with adsorption capacity of 330 mg g(-1) resin. Through the column treatment, the complete removal of large amounts of alkali and alkaline earth matrices without any loss of adsorption efficiency over prolonged usage were achieved with this resin. The CCTS-DHBA resin was applied to the adsorption/collection of uranium in tap water, river water and seawater samples with satisfactory results. The validation of the proposed method was carried out by analyzing uranium in the standard reference materials of SLRS-4, CASS-4, and NASS-5 after passing through the CCTS-DHBA resin, and the results showed good agreement with the certified values. en-copyright= kn-copyright= en-aut-name=SabarudinAkhmad en-aut-sei=Sabarudin en-aut-mei=Akhmad kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OshimaMitsuko en-aut-sei=Oshima en-aut-mei=Mitsuko kn-aut-name=大島光子 kn-aut-sei=大島 kn-aut-mei=光子 aut-affil-num=2 ORCID= en-aut-name=TakayanagiToshio en-aut-sei=Takayanagi en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HakimLukman en-aut-sei=Hakim en-aut-mei=Lukman kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OshitaKoji en-aut-sei=Oshita en-aut-mei=Koji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=GaoYun Hua en-aut-sei=Gao en-aut-mei=Yun Hua kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MotomizuShoji en-aut-sei=Motomizu en-aut-mei=Shoji kn-aut-name=本水昌二 kn-aut-sei=本水 kn-aut-mei=昌二 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=4 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=5 en-affil= kn-affil=Department of International Conservation Studies, Faculty of International and Industrial Studies, Kibi International University affil-num=6 en-affil= kn-affil=Technical Institute of Physics and Chemistry, Chinese Academy of Sciences affil-num=7 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University en-keyword=Chitosan resin kn-keyword=Chitosan resin en-keyword=3,4-dihydroxybenzoic acid kn-keyword=3,4-dihydroxybenzoic acid en-keyword=Uranium kn-keyword=Uranium en-keyword=Adsorption kn-keyword=Adsorption en-keyword=Water kn-keyword=Water en-keyword=Inductively coupled plasma-mass spectrometry kn-keyword=Inductively coupled plasma-mass spectrometry END start-ver=1.4 cd-journal=joma no-vol=588 cd-vols= no-issue=1 article-no= start-page=73 end-page=81 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=20070404 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Synthesis of novel chitosan resin derivatized with serine diacetic acid moiety and its application to on-line collection/concentration of trace elements and their determination using inductively coupled plasma-atomic emission spectrometry en-subtitle= kn-subtitle= en-abstract= kn-abstract=A novel chelating resin functionalized with serine diacetic acid moiety was synthesized by using chitosan as base material, and applied to the collection/concentration of trace elements in environmental water samples, followed by the determination using inductively coupled plasma-atomic emission spectrometer (ICP-AES). The synthesized resin, crosslinked chitosan serine diacetic acid (CCTS-SDA), showed good adsorption behavior toward trace amounts of Cd, Pb, Cu, Ni, V, Ga, Sc, In, and Th in a wide pH range. Additionally, rare earth elements also can be retained on the resin at neutral pH region. The adsorbed elements can be easily eluted with 1 mol L-1 of nitric acid, and their recoveries were found to be 90-100%. The CCTS-SDA was packed in a mini-column, which was then installed in a cornputer-controlled auto-pretreatment system (Auto-Pret System) for on-line trace elements collection and determination with ICP-AES. Experimental parameters which related to the improvement of sensitivity and reproducibility were optimized. The limits of detection (LOD) for 13 elements were found to be in sub-ppb level. The proposed method with CCTS-SDA resin was successfully applied to the determination of trace elements in river water samples. The method was validated by determining a certified reference material of river water, SLRS-4. en-copyright= kn-copyright= en-aut-name=LukmanHakim en-aut-sei=Lukman en-aut-mei=Hakim kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SabarudinAkhmad en-aut-sei=Sabarudin en-aut-mei=Akhmad kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OshimaMitsuko en-aut-sei=Oshima en-aut-mei=Mitsuko kn-aut-name=大島光子 kn-aut-sei=大島 kn-aut-mei=光子 aut-affil-num=3 ORCID= en-aut-name=MotomizuShoji en-aut-sei=Motomizu en-aut-mei=Shoji kn-aut-name=本水昌二 kn-aut-sei=本水 kn-aut-mei=昌二 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=4 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University en-keyword=chelating resin kn-keyword=chelating resin en-keyword=chitosan kn-keyword=chitosan en-keyword=serine diacetic acid moiety kn-keyword=serine diacetic acid moiety en-keyword=trace elements kn-keyword=trace elements en-keyword=on-line pretreatment kn-keyword=on-line pretreatment en-keyword=computer control kn-keyword=computer control en-keyword=inductively coupled plasma-atomic emission spectrometry kn-keyword=inductively coupled plasma-atomic emission spectrometry en-keyword=SLRS-4 kn-keyword=SLRS-4 END start-ver=1.4 cd-journal=joma no-vol=612 cd-vols= no-issue=2 article-no= start-page=165 end-page=172 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=200804 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Development of novel detection reagent for simple and sensitive determination of trace amounts of formaldehyde and its application to flow injection spectrophotometric analysis en-subtitle= kn-subtitle= en-abstract= kn-abstract=In this paper, a novel detection reagent for formaldehyde determination is proposed, and is applied to a simple and highly sensitive flow injection method for the spectrophotometric determination of formaldehyde. The method is based on the reaction of formaldehyde with methyl acetoacetate in the presence of ammonia. The increase in the absorbance of the reaction product was measured at 375 nm. An inexpensive light emitting diode (LED)based UV detector (375 nm) was, for the first time, used. Under the optimized experimental conditions, formaldehyde in an aqueous solution was determined over the concentration range from 0.25 to 20.0 x 10(-6) M with a liner calibration graph; the limit of detection (LOD) of 5 x 10(-8) M (1.5 mu g L-1) was possible. The relative standard deviation of 12 replicate measurements of 5 X 10(-6) M formaldehyde was 1.2%. Maximum sampling throughput was about 21 samples/h. The effect of potential interferences such as metals, organic compounds and other aldehyde was also examined. The analytical performance for formaldehyde determination was compared with those obtained by the conventional acetylacetone method, which uses visible absorption spectrophotometry. Finally, the proposed method was successfully applied to the determination of formaldehyde in natural water samples. en-copyright= kn-copyright= en-aut-name=LiQiong en-aut-sei=Li en-aut-mei=Qiong kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SritharathikhumPiyanete en-aut-sei=Sritharathikhum en-aut-mei=Piyanete kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OshimaMitsuko en-aut-sei=Oshima en-aut-mei=Mitsuko kn-aut-name=大島光子 kn-aut-sei=大島 kn-aut-mei=光子 aut-affil-num=3 ORCID= en-aut-name=MotomizuShoji en-aut-sei=Motomizu en-aut-mei=Shoji kn-aut-name=本水昌二 kn-aut-sei=本水 kn-aut-mei=昌二 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=3 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University affil-num=4 en-affil= kn-affil=Department of Chemistry, Faculty of Science, Okayama University en-keyword=Flow injection kn-keyword=Flow injection en-keyword=Formaldehyde determination kn-keyword=Formaldehyde determination en-keyword=Spectrophotometry kn-keyword=Spectrophotometry en-keyword=Methyl acetoacetate kn-keyword=Methyl acetoacetate en-keyword=UV-light emitting diode (LED) kn-keyword=UV-light emitting diode (LED) END start-ver=1.4 cd-journal=joma no-vol=620 cd-vols= no-issue=1-2 article-no= start-page=50 end-page=54 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080714 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A caffeine-sensitive membrane electrode: Previous misleading report and present approach en-subtitle= kn-subtitle= en-abstract= kn-abstract=Although a previous study [S.S.M. Hassan, M.A. Ahmed, M.M. Saoudi, Anal. Chem. 57 (1985) 1126] had shown that a caffeine-sensitive electrode made with picrylsulfonate and 1-octanol as a cation-exchanger and a solvent mediator, respectively, had a wide working pH range (5.5-9.5) and exhibited a Nernstian response, we could not find such response in this electrode. The present result was reasonable, because the pK, value of caffeinium ion was reported to be around 0.7 and the neutral form of caffeine was predominant in the pH range examined. Thus, we reinvestigated the response characteristics of a caffeine electrode, taking into consideration the pKa value, and constructed a new electrode with a combination of the lipophilic cation-exchanger, tetrakis[3,5-bis(2-methoxyhexafluoro-2-propyl)phenyl]borate (HFPB), and the solvent mediator with high degree of dielectric constant, 2-fluoro-2'-nitrodiphenyl ether (FNDPE). This electrode showed a pH-dependent response to caffeinium ion and gave a detection limit of 50 mu M with a slope of 55 mV per concentration decade at pH 2. The use of other solvent mediators was less effective than that of FNDPE. The electrode was applied for the determination of caffeine in some central stimulants. en-copyright= kn-copyright= en-aut-name=KatsuTakashi en-aut-sei=Katsu en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsunamotoYumi en-aut-sei=Tsunamoto en-aut-mei=Yumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HaniokaNobumitsu en-aut-sei=Hanioka en-aut-mei=Nobumitsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KomagoeKeiko en-aut-sei=Komagoe en-aut-mei=Keiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MasudaKazufumi en-aut-sei=Masuda en-aut-mei=Kazufumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NarimatsuShizuo en-aut-sei=Narimatsu en-aut-mei=Shizuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=2 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=3 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=4 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=5 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=6 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University en-keyword=ion-selective electrode kn-keyword=ion-selective electrode en-keyword=caffeinium ion kn-keyword=caffeinium ion en-keyword=solvent mediator kn-keyword=solvent mediator en-keyword=2-fluoro-2 '-nitrodiphenyl ether kn-keyword=2-fluoro-2 '-nitrodiphenyl ether en-keyword=caffeine determination kn-keyword=caffeine determination END start-ver=1.4 cd-journal=joma no-vol=122 cd-vols= no-issue=2 article-no= start-page=219 end-page=226 dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=20090221 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Promotion of IL-4- and IL-5-dependent differentiation of anti-μ-primed B cells by ascorbic acid 2-glucoside en-subtitle= kn-subtitle= en-abstract= kn-abstract=The stable ascorbic acid derivative 2-O-alpha-D-glucopyranosyl-L-ascorbic acid (AA-2G) was used to investigate the role of ascorbic acid (AA) in B cell differentiation in vitro. AA-2G is stable in a solution unlike AA but is hydrolyzed by cellular alpha-glucosidase to release AA. Mouse spleen B cells were primed for 2 days with an anti-mu antibody in the presence of interleukin (IL)-4 and IL-5 and then washed and recultured with AA-2G in the presence of IL-4 and IL-5. AA-2G, but not AA, dose-dependently increased IgM production, the greatest enhancement being 150% at concentrations of more than 0.5 mM. In the absence of IL-4 and IL-5, primed B cells produced a negligible amount of IgM, and AA-2G had no effect. AA-2G-induced IgM production in the presence of IL-4 and IL-5 was inhibited by the alpha-glucosidase inhibitor castanospermine. Intracellular AA content, depleted during the priming period, increased by adding AA-2G at the start of reculture. Treatment of B cells with AA-2G resulted in an increase in the number of IgM-secreting cells, CD138-positive cells and CD45R/B220-negative cells. The number of viable cells in untreated cultures decreased gradually, but the decrease was significantly attenuated by AA-2G, resulting in about 70% more viable cells in AA-2G-treated cultures. AA-2G caused a slight but reproducible enhancement of DNA synthesis and a slight decrease in the number of cells with a sub-G1 DNA content. These results demonstrated that AA released from AA-2G enhanced cytokine-dependent IgM production in anti-mu-primed B cells and suggest that its effect is caused through promoting the differentiation of B cells to plasma cells and attenuating the gradual decrease in the number of viable cells. en-copyright= kn-copyright= en-aut-name=IchiyamaKenji en-aut-sei=Ichiyama en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MitsuzumiHitoshi en-aut-sei=Mitsuzumi en-aut-mei=Hitoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ZhongMing en-aut-sei=Zhong en-aut-mei=Ming kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TaiAkihiro en-aut-sei=Tai en-aut-mei=Akihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TsuchiokaAkihiro en-aut-sei=Tsuchioka en-aut-mei=Akihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KawaiSaeko en-aut-sei=Kawai en-aut-mei=Saeko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=YamamotoItaru en-aut-sei=Yamamoto en-aut-mei=Itaru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=GohdaEiichi en-aut-sei=Gohda en-aut-mei=Eiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=5 en-affil= kn-affil=Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=7 en-affil= kn-affil=Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=8 en-affil= kn-affil=Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=2-O-α-D-Glucopyranosyl-l-ascorbic acid (AA-2G) kn-keyword=2-O-α-D-Glucopyranosyl-l-ascorbic acid (AA-2G) en-keyword=Ascorbic acid kn-keyword=Ascorbic acid en-keyword=Anti-μ antibody kn-keyword=Anti-μ antibody en-keyword=IgM production kn-keyword=IgM production en-keyword=B cell differentiation kn-keyword=B cell differentiation END start-ver=1.4 cd-journal=joma no-vol=1797 cd-vols= no-issue=2 article-no= start-page=278 end-page=284 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=201002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Structural and functional studies on Ycf12 (Psb30) and PsbZ-deletion mutants from a thermophilic cyanobacterium en-subtitle= kn-subtitle= en-abstract= kn-abstract=Ycf12 (Psb30) and PsbZ are two low molecular weight subunits of photosystem II (PSII), with one and two trans-membrane helices, respectively. In order to study the functions of these two subunits from a structural point of view, we constructed deletion mutants lacking either Ycf12 or PsbZ from Thermosynechococcus elongatus, and purified, crystallized and analyzed the structure of PSII dimer from the two mutants. Our results showed that Ycf12 is located in the periphery of PSII, close to PsbK, PsbZ and PsbJ, and corresponded to the unassigned helix X1 reported previously, in agreement with the recent structure at 2.9 ? resolution (A. Guskov, J. Kern, A. Gabdulkhakov, M. Broser, A. Zouni, W. Saenger, Cyanobacterial photosystem II at 2.9 ? resolution: role of quinones, lipids, channels and chloride, Nat. Struct. Mol. Biol. 16 (2009) 334?342). On the other hand, crystals of PsbZ-deleted PSII showed a remarkably different unit cell constants from those of wild-type PSII, indicating a role of PsbZ in the interactions between PSII dimers within the crystal. This is the first example for a different arrangement of PSII dimers within the cyanobacterial PSII crystals. PSII dimers had a lower oxygen-evolving activity from both mutants than that from the wild type. In consistent with this, the relative content of PSII in the thylakoid membranes was lower in the two mutants than that in the wild type. These results suggested that deletion of both subunits affected the PSII activity, thereby destabilized PSII, leading to a decrease in the PSII content in vivo. While PsbZ was present in PSII purified from the Ycf12-deletion mutant, Ycf12 was present in crude PSII but absent in the finally purified PSII from the PsbZ-deletion mutant, indicating a preferential, stabilizing role of PsbZ for the binding of Ycf12 to PSII. These results were discussed in terms of the PSII crystal structure currently available en-copyright= kn-copyright= en-aut-name=TakasakaKenji en-aut-sei=Takasaka en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IwaiMasako en-aut-sei=Iwai en-aut-mei=Masako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=UmenaYasufumi en-aut-sei=Umena en-aut-mei=Yasufumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KawakamiKeisuke en-aut-sei=Kawakami en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OhmoriYukari en-aut-sei=Ohmori en-aut-mei=Yukari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=IkeuchiMasahiko en-aut-sei=Ikeuchi en-aut-mei=Masahiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TakahashiYuichiro en-aut-sei=Takahashi en-aut-mei=Yuichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KamiyaNobuo en-aut-sei=Kamiya en-aut-mei=Nobuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=ShenJian-Ren en-aut-sei=Shen en-aut-mei=Jian-Ren kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology/Faculty of Science; Okayama University affil-num=2 en-affil= kn-affil=Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science affil-num=3 en-affil= kn-affil=Department of Chemistry, Graduate School of Science, Osaka City University affil-num=4 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology/Faculty of Science; Okayama University affil-num=5 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology/Faculty of Science; Okayama University affil-num=6 en-affil= kn-affil=Department of Life Sciences (Biology), Graduate School of Arts and Science, The University of Tokyo affil-num=7 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology/Faculty of Science; Okayama University affil-num=8 en-affil= kn-affil=Department of Chemistry, Graduate School of Science, Osaka City University affil-num=9 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology/Faculty of Science; Okayama University en-keyword=Photosystem II kn-keyword=Photosystem II en-keyword=Mutant kn-keyword=Mutant en-keyword=Crystal structure kn-keyword=Crystal structure en-keyword=Ycf12 kn-keyword=Ycf12 en-keyword=PsbZ kn-keyword=PsbZ en-keyword=Oxygen evolution kn-keyword=Oxygen evolution END start-ver=1.4 cd-journal=joma no-vol=1808 cd-vols= no-issue=1 article-no= start-page=490 end-page=497 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=201101 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Comparative study of the membrane-permeabilizing activities of mastoparans and related histamine-releasing agents in bacteria, erythrocytes, and mast cells en-subtitle= kn-subtitle= en-abstract= kn-abstract=The membrane-permeabilizing activities of mastoparans and related histamine-releasing agents were compared through measurements of K(+) efflux from bacteria, erythrocytes, and mast cells. Changes in bacterial cell viability, hemolysis, and histamine release, as well as in the shape of erythrocytes were also investigated. The compounds tested were mastoparans (HR1, a mastoparan from Polistes jadwagae, and a mastoparan from Vespula lewisii), granuliberin R, mast cell-degranulating peptide, and compound 48/80, as well as antimicrobial peptides, such as magainin I, magainin II, gramicidin S. and melittin. We used a K(+)-selective electrode to determine changes in the permeability to K(+) of the cytoplasmic membranes of cells. Consistent with the surface of mast cells becoming negatively charged during histamine release, due to the translocation of phosphatidylserine to the outer leaflet of the cytoplasmic membrane, histamine-releasing agents induced K(+) efflux from mast cells, dependent on their ability to increase the permeability of bacterial cytoplasmic membranes rich in negatively charged phospholipids. The present results demonstrated that amphiphilic peptides, possessing both histamine-releasing and antimicrobial capabilities, induced the permeabilization of the cytoplasmic membranes of not only bacteria but mast cells. Mastoparans increased the permeability of membranes in human erythrocytes at higher concentrations, and changed the normal discoid shape to a crenated form. The structural requirement for making the crenated form was determined using compound 48/80 and its constituents (monomer, dimer, and trimer), changing systematically the number of cationic charges of the molecules. en-copyright= kn-copyright= en-aut-name=NakaoSatoshi en-aut-sei=Nakao en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KomagoeKeiko en-aut-sei=Komagoe en-aut-mei=Keiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=InoueTsuyoshi en-aut-sei=Inoue en-aut-mei=Tsuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KatsuTakashi en-aut-sei=Katsu en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=2 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=3 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University affil-num=4 en-affil= kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University en-keyword=Mastoparan kn-keyword=Mastoparan en-keyword=Compound 48/80 kn-keyword=Compound 48/80 en-keyword=Antimicrobial peptide kn-keyword=Antimicrobial peptide en-keyword=Histamine-releasing agent kn-keyword=Histamine-releasing agent en-keyword=Membrane permeability kn-keyword=Membrane permeability en-keyword=Cell shape kn-keyword=Cell shape END start-ver=1.4 cd-journal=joma no-vol=102 cd-vols= no-issue=3-4 article-no= start-page=620 end-page=626 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=20110222 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Improvement in sulfur desorption of NOX storage and reduction catalysts using a Ba?Ti composite oxide en-subtitle= kn-subtitle= en-abstract= kn-abstract=A Ba-Ti composite oxide was formed on a NOx storage and reduction catalyst via impregnation of a Ba-Ti precursor solution composed of H(2)O(2) added to a complex prepared using the citric acid method. The structure of the Ba-Ti composite in solution was analyzed by chemical composition analysis and FT-Raman and UV-vis spectroscopy. MM2 calculations were performed to propose its chemical structure. Both Ba and Ti together were found to form a composite molecule in the solution. Furthermore, TEM-EDX and XRD analyses of the Ba-Ti composite oxide on the catalyst prepared by impregnation with the Ba-Ti composite aqueous solution revealed that Ba and Ti in the catalyst were highly dispersed at the nm scale. The formation of the Ba-Ti composite oxide on the NSR catalyst enhanced sulfur desorption efficiency and led to high-performance NO(X) conversion as a NO(X) storage and reduction activity catalyst after desulfation treatment. It was assumed that the existence of nano-scaled Ba compounds combined with Ti was efficient for the inhibition of the sintering of barium sulfate and its facile decomposition. It was found that dispersion of Ba compounds for NO(X) storage materials using a Ba-Ti complex solution is an efficient way to improve the durability of NSR catalysts. en-copyright= kn-copyright= en-aut-name=TanakaToshiyuki en-aut-sei=Tanaka en-aut-mei=Toshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TajimaIchirou en-aut-sei=Tajima en-aut-mei=Ichirou kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KatoYuichi en-aut-sei=Kato en-aut-mei=Yuichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NishiharaYasushi en-aut-sei=Nishihara en-aut-mei=Yasushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ShinjohHirofumi en-aut-sei=Shinjoh en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Toyota Central Research and Development Labs., Inc. affil-num=2 en-affil= kn-affil=Toyota Central Research and Development Labs., Inc. affil-num=3 en-affil= kn-affil=Toyota Central Research and Development Labs., Inc. affil-num=4 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=5 en-affil= kn-affil=Toyota Central Research and Development Labs., Inc. END start-ver=1.4 cd-journal=joma no-vol=1807 cd-vols= no-issue=3 article-no= start-page=319 end-page=325 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=201103 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Roles of PsbI and PsbM in photosystem II dimer formation and stability studied by deletion mutagenesis and X-ray crystallography en-subtitle= kn-subtitle= en-abstract= kn-abstract=PsbM and PsbI are two low molecular weight subunits of photosystem II (PSII), with PsbM being located in the center, and PsbI in the periphery, of the PSII dimer. In order to study the functions of these two subunits from a structural point of view, we crystallized and analyzed the crystal structure of PSII dimers from two mutants lacking either PsbM or PsbI. Our results confirmed the location of these two subunits in the current crystal structure, as well as their absence in the respective mutants. The relative contents of PSII dimers were found to be decreased in both mutants, with a concomitant increase in the amount of PSII monomers, suggesting a destabilization of PSII dimers in both of the mutants. On the other hand, the accumulation level of the overall PSII complexes in the two mutants was similar to that in the wild-type strain. Treatment of purified PSII dimers with lauryldimethylamine N-oxide at an elevated temperature preferentially disintegrated the dimers from the PsbM deletion mutant into monomers and CP43-less monomers, whereas no significant degradation of the dimers was observed from the PsbI deletion mutant. These results indicate that although both PsbM and PsbI are required for the efficient formation and stability of PSII dimers in vivo, they have different roles, namely, PsbM is required directly for the formation of dimers and its absence led to the instability of the dimers accumulated. On the other hand, PsbI is required in the assembly process of PSII dimers in vivo; once the dimers are formed, PsbI was no longer required for its stability. en-copyright= kn-copyright= en-aut-name=KawakamiKeisuke en-aut-sei=Kawakami en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=UmenaYasufumi en-aut-sei=Umena en-aut-mei=Yasufumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=IwaiMasako en-aut-sei=Iwai en-aut-mei=Masako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KawabataYousuke en-aut-sei=Kawabata en-aut-mei=Yousuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=IkeuchiMasahiko en-aut-sei=Ikeuchi en-aut-mei=Masahiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KamiyaNobuo en-aut-sei=Kamiya en-aut-mei=Nobuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=ShenJian-Ren en-aut-sei=Shen en-aut-mei=Jian-Ren kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology/Faculty of Science, Okayama University affil-num=2 en-affil= kn-affil=Department of Chemistry, Graduate School of Science, Osaka City University affil-num=3 en-affil= kn-affil=Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science affil-num=4 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology/Faculty of Science, Okayama University affil-num=5 en-affil= kn-affil=Department of Life Sciences (Biology), Graduate School of Arts and Science, The University of Tokyo affil-num=6 en-affil= kn-affil=Department of Chemistry, Graduate School of Science, Osaka City University affil-num=7 en-affil= kn-affil=Division of Bioscience, Graduate School of Natural Science and Technology/Faculty of Science, Okayama University en-keyword=Photosystem II kn-keyword=Photosystem II en-keyword=Mutant kn-keyword=Mutant en-keyword=Crystal structure kn-keyword=Crystal structure en-keyword=PsbM kn-keyword=PsbM en-keyword=PsbI kn-keyword=PsbI en-keyword=Oxygen evolution kn-keyword=Oxygen evolution END start-ver=1.4 cd-journal=joma no-vol=164 cd-vols= no-issue=1 article-no= start-page=552 end-page=555 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=20110430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=2-Diphenylphosphanyl-4-pyridyl(dimethyl) amine as an effective ligand for the ruthenium(II) complex catalyzed homogeneous hydration of nitriles under neutral conditions en-subtitle= kn-subtitle= en-abstract= kn-abstract=New homogeneous catalyst comprised of [Ru(methallyl)(2)(cod)] (cod = 1,5-cyclooctadiene) (1) and 2-diphenylphosphanyl-4-pyridyl(dimethyl) amine (2) is shown to efficiently catalyze the hydration of various nitriles under neutral conditions. The hydration proceeds in the presence of 0.5 mol% of the ruthenium catalyst at 80 degrees C in 1,2-dimethoxyethane solution and the corresponding amide is obtained within few hours without the formation of by products. Comparison of some phosphine ligands for the hydration reveals that the dimethylamino moiety of 2 improves the catalytic performance dramatically. en-copyright= kn-copyright= en-aut-name=MuranakaMakoto en-aut-sei=Muranaka en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HyodoIsao en-aut-sei=Hyodo en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OkumuraWataru en-aut-sei=Okumura en-aut-mei=Wataru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OshikiToshiyuki en-aut-sei=Oshiki en-aut-mei=Toshiyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil= kn-affil=Division of Chemistry and Biochemistry, Graduate School of Natural Science and Technology, Okayama University en-keyword=Hydration kn-keyword=Hydration en-keyword=Nitrile kn-keyword=Nitrile en-keyword=Amides kn-keyword=Amides en-keyword=Homogeneous catalysis kn-keyword=Homogeneous catalysis en-keyword=Ruthenium kn-keyword=Ruthenium en-keyword=Phosphine kn-keyword=Phosphine END start-ver=1.4 cd-journal=joma no-vol=30 cd-vols= no-issue=4 article-no= start-page=258 end-page=266 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=201105 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Drosophila type XV/XVIII collagen, Mp, is involved in Wingless distribution en-subtitle= kn-subtitle= en-abstract= kn-abstract=Multiplexin (Mp) is the Drosophila orthologue of vertebrate collagens XV and XVIII. Like them, Mp is widely distributed in the basement membranes of the developing embryos, including those of neuroblasts in the central and peripheral nervous systems, visceral muscles of the gut, and contractile cardioblasts. Here we report the identification of mutant larvae bearing piggyBac transposon insertions that exhibit decrease Mp production associated with abdominal cuticular and wing margin defects, malformation of sensory organs and impaired sensitivity to physical stimuli. Additional findings include the abnormal ultrastructure of fatbody associated with abnormal collagen IV deposition, and reduced Wingless deposition. Collectively, these findings are consistent with the notion that Mp is required for the proper formation and/or maintenance of basement membrane, and that Mp may be involved in establishing the Wingless signaling gradients in the Drosophila embryo. en-copyright= kn-copyright= en-aut-name=MomotaRyusuke en-aut-sei=Momota en-aut-mei=Ryusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NaitoIchiro en-aut-sei=Naito en-aut-mei=Ichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NinomiyaYoshifumi en-aut-sei=Ninomiya en-aut-mei=Yoshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OhtsukaAiji en-aut-sei=Ohtsuka en-aut-mei=Aiji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Department of Human Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Human Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Human Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=Extracellular matrix kn-keyword=Extracellular matrix en-keyword=Basement membrane kn-keyword=Basement membrane en-keyword=Collagen kn-keyword=Collagen en-keyword=Proteoglycan kn-keyword=Proteoglycan en-keyword=Chondroitin sulfate kn-keyword=Chondroitin sulfate en-keyword=Wingless/Wnt kn-keyword=Wingless/Wnt END start-ver=1.4 cd-journal=joma no-vol=1218 cd-vols= no-issue=25 article-no= start-page=3923 end-page=3927 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=20110624 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Rapid determination of multidrug resistance-associated protein in cancer cells by capillary electrophoresis immunoassay en-subtitle= kn-subtitle= en-abstract= kn-abstract=The adenosine triphosphate (ATP) binding-cassette (ABC) transporters are a superfamily of cellular proteins that have been partly implicated as a cause of multidrug resistance (MDR) in cancer cells. The ABC superfamily consists of P-glycoprotein, multidrug resistance-associated proteins (MRP) and breast cancer-related proteins, of which MRP is of particular interest because of its ability to efflux a broader range of substrates. Since MRP1 is the most prominent member of the MRP family, a simple technique is needed for its quantification. We developed a simple, fast (total analysis time of 3 h) capillary electrophoresis immunoassay (CEIA) for the quantification of MRP1 in cancer cells. MRP1 antibody was labeled with fluorescein isothiocyanate. The labeled antibody was incubated with the cell lysate for a fixed interval (1 h), after which the cell lysate mixture was directly injected into the capillary to separate the complex of MRP1 and its antibody from free antibody. The noncompetitive CEIA method had a limit of detection of 0.2 nM and a good linear range (1.7-14.9 x 10(4) cells), and was fairly reproducible (RSD < 10%). The results showed that two cell lines. A549 and RDES, expressed MRP1 in the absence of doxorubicin (DOX), with A549 registering a higher expression. Compared to DOX-free cancer cells, there was an acceleration of MRP1 expression during the 12 h-exposure to DOX, after which the level of expression remained nearly constant as the intracellular accumulation of DOX decreased. The results obtained in this work indicate that the developed CEIA method is useful for relative quantification of MRPs in cancer cells. en-copyright= kn-copyright= en-aut-name=MbunaJulius en-aut-sei=Mbuna en-aut-mei=Julius kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KanetaTakashi en-aut-sei=Kaneta en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ImasakaTotaro en-aut-sei=Imasaka en-aut-mei=Totaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Applied Chemistry, Graduate School of Engineering, Kyushu University affil-num=2 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Department of Applied Chemistry, Graduate School of Engineering, Kyushu University en-keyword=Capillary electrophoresis immunoassay kn-keyword=Capillary electrophoresis immunoassay en-keyword=Multidrug resistance-associated protein kn-keyword=Multidrug resistance-associated protein en-keyword=Cancer cell kn-keyword=Cancer cell en-keyword=Laser-induced fluorescence kn-keyword=Laser-induced fluorescence en-keyword=Doxorubicin kn-keyword=Doxorubicin END start-ver=1.4 cd-journal=joma no-vol=65 cd-vols= no-issue=14 article-no= start-page=2197 end-page=2200 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=20110731 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Effect of varying the ratio of matrix/dispersoid particle size on the piezoresistivity of alumina/carbon-black composite ceramics en-subtitle= kn-subtitle= en-abstract= kn-abstract=Alumina/carbon-black composite ceramics with different percolation thresholds were fabricated by changing the size ratio of constituent particles. The dependence of resistivity on pressure was established for each sample. The compositional dependence of resistivity can be explained by percolation theory. The percolation threshold decreases with increasing alumina/carbon-black particle size ratio. The pressure dependence of the resistivity increases as the composition approaches the percolation threshold. When the relative composition at the percolation threshold is fixed, the sensitivity increases with increasing matrix/dispersoid initial particle size ratio. en-copyright= kn-copyright= en-aut-name=KishimotoAkira en-aut-sei=Kishimoto en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakagawaYuto en-aut-sei=Takagawa en-aut-mei=Yuto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TeranishiTakashi en-aut-sei=Teranishi en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=HayashiHidetaka en-aut-sei=Hayashi en-aut-mei=Hidetaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Division of Molecular and Material Science, Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Division of Molecular and Material Science, Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Division of Molecular and Material Science, Graduate School of Natural Science and Technology, Okayama University affil-num=4 en-affil= kn-affil=Division of Molecular and Material Science, Graduate School of Natural Science and Technology, Okayama University en-keyword=Percolation kn-keyword=Percolation en-keyword=Piezoresistivity kn-keyword=Piezoresistivity en-keyword=Pressure sensor kn-keyword=Pressure sensor en-keyword=Particle size kn-keyword=Particle size END start-ver=1.4 cd-journal=joma no-vol=203 cd-vols= no-issue=1 article-no= start-page=157 end-page=162 dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20120115 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Mechanomyographic activity in the human lateral pterygoid muscle during mandibular movement en-subtitle= kn-subtitle= en-abstract= kn-abstract=The activity of the lateral pterygoid muscle has been regarded to be related to the pathological condition of the temporomandibular joint (TMJ) in the craniomandibular disorders. Because the lateral pterygoid muscle is a deep muscle, a needle electrode is necessary for EMG recordings. The purpose of this study was to establish a non-invasive method for the evaluation of muscle activity of the lateral pterygoid muscle using mechanomyogram (MMG). In three male subjects, surface electromyogram (EMG) in the left masseter muscle, left anterior and posterior belly of the temporal muscle, left anterior belly of the digastric muscle and needle EMG of the inferior head of the lateral pterygoid were recorded during mandibular movement tasks simultaneously with the MMG derived from a condenser microphone in the external ear canal. There were significant positive correlations between the needle EMG signal of the lateral pterygoid muscle and the MMG signal for the tasks of static jaw opened position of 30 mm of interincisal distance (p = 0.000, R(2)=0.725), static jaw opened position of 40 mm of interincisal distance (p = 0.000, R(2) = 0.753), 5 mm protruded mandibular position (p = 0.000, R(2) = 0.653), the most protruded mandibular position (p = 0.000, R(2) = 0803). On the contrary, for the task of maximal clenching, there was no significant correlation between the EMG signal of the lateral pterygoid muscle and the MMG signal. These results suggest that the activity of the lateral pterygoid muscle could be evaluated by the MMG signals recorded in the external ear canal, unless jaw closing major muscles show active contraction. en-copyright= kn-copyright= en-aut-name=KawakamiShigehisa en-aut-sei=Kawakami en-aut-mei=Shigehisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KodamaNaoki en-aut-sei=Kodama en-aut-mei=Naoki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MaedaNaoto en-aut-sei=Maeda en-aut-mei=Naoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SakamotoShunichi en-aut-sei=Sakamoto en-aut-mei=Shunichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OkiKazuhiro en-aut-sei=Oki en-aut-mei=Kazuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YanagiYoshinobu en-aut-sei=Yanagi en-aut-mei=Yoshinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=AsaumiJun-Ichi en-aut-sei=Asaumi en-aut-mei=Jun-Ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=MaedaTeruta en-aut-sei=Maeda en-aut-mei=Teruta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=MinagiShogo en-aut-sei=Minagi en-aut-mei=Shogo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Okayama Univ, Dept Occlusal & Oral Funct Rehabil affil-num=2 en-affil= kn-affil=Okayama Univ, Dept Occlusal & Oral Funct Rehabil affil-num=3 en-affil= kn-affil=Okayama Univ, Dept Occlusal & Oral Funct Rehabil affil-num=4 en-affil= kn-affil=Okayama Univ, Dept Occlusal & Oral Funct Rehabil affil-num=5 en-affil= kn-affil=Okayama Univ, Dept Occlusal & Oral Funct Rehabil affil-num=6 en-affil= kn-affil=Okayama Univ, Dept Oral & Maxillofacial Radiol affil-num=7 en-affil= kn-affil=Okayama Univ, Dept Oral & Maxillofacial Radiol affil-num=8 en-affil= kn-affil=Osaka Dent Univ, Dept Postgrad Clin Training affil-num=9 en-affil= kn-affil=Osaka Dent Univ, Dept Postgrad Clin Training en-keyword=Mechanomyogram kn-keyword=Mechanomyogram en-keyword=Electromyogram kn-keyword=Electromyogram en-keyword=Lateral pterygoid muscle kn-keyword=Lateral pterygoid muscle en-keyword=Jaw movement kn-keyword=Jaw movement END start-ver=1.4 cd-journal=joma no-vol=1288 cd-vols= no-issue= article-no= start-page=149 end-page=154 dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=20130503 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Determination of association constants between 5 '-guanosine monophosphate gel and aromatic compounds by capillary electrophoresis en-subtitle= kn-subtitle= en-abstract= kn-abstract=Hydro gel formed by 5'-guanosine monophosphate (GMP) in the presence of a potassium ion is expected to exhibit interesting selectivity in capillary electrophoretic separations. Here, we estimated the conditional association constants between the hydro gel (G-gel) and aromatic compounds by capillary electrophoresis in order to investigate the separation selectivity that is induced by the G-gel. Several aromatic compounds were separated in a solution containing GMP and potassium ion at different concentrations. The association constants were calculated by correlating the electrophoretic mobilities of the analytes obtained experimentally using a concentration of G-gel. During semi-quantitative estimation, naphthalene derivatives had larger association constants (K-ass = 10.3-16.8) compared with those of benzene derivatives (K-ass = 3.91-5.31), which means that the binding sites of G-gel match better to a naphthalene ring than to a benzene ring. A hydrophobic interaction was also found when the association constants for alkyl resorcinol were compared with those of different hydrocarbon chains. The association constants of nucleobases and tryptophan ranged from 6.05 to 12.6, which approximated the intermediate values between benzene and naphthalene derivatives. Consequently, the selective interaction between G-gel and aromatic compounds was classified as one of three types: (1) an intercalation into stacked planar GMP tetramers; (2) a hydrophobic interaction with a long alkyl chain; or, (3) a small contribution of steric hindrance and/or hydrogen bonding with functional groups such as amino and hydroxyl groups. en-copyright= kn-copyright= en-aut-name=YamaguchiKaori en-aut-sei=Yamaguchi en-aut-mei=Kaori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakeyasuNobuyuki en-aut-sei=Takeyasu en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KanetaTakashi en-aut-sei=Kaneta en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama Univ, Grad Sch Nat Sci & Technol, Div Earth Life & Mol Sci, Dept Chem affil-num=2 en-affil= kn-affil=Okayama Univ, Grad Sch Nat Sci & Technol, Div Earth Life & Mol Sci, Dept Chem affil-num=3 en-affil= kn-affil=Okayama Univ, Grad Sch Nat Sci & Technol, Div Earth Life & Mol Sci, Dept Chem en-keyword=Capillary electrophoresis kn-keyword=Capillary electrophoresis en-keyword=5 '-Guanosine monophosphate (GMP) kn-keyword=5 '-Guanosine monophosphate (GMP) en-keyword=G-gel kn-keyword=G-gel en-keyword=Association constant kn-keyword=Association constant END start-ver=1.4 cd-journal=joma no-vol=156 cd-vols= no-issue=1-2 article-no= start-page=82 end-page=93 dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=201312 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=IL-7 promotes long-term in vitro survival of unique long-lived memory subset generated from mucosal effector memory CD4(+) T cells in chronic colitis mice en-subtitle= kn-subtitle= en-abstract= kn-abstract=Colitogenic memory CD4(+) T cells are important in the pathogenesis of inflammatory bowel disease (IBD). Although memory stem cells with high survival and self-renewal capacity were recently identified in both mice and humans, it is unclear whether a similar subset is present in chronic colitis mice. We sought to identify and purify a long-lived subset of colitogenic memory CD4(+) T cells, which may be targets for treatment of IBD. A long-lived subset of colitogenic memory CD4(+) T cells was purified using a long-term culture system. The characteristics of these cells were assessed. Interleukin (IL)-7 promoted the in vitro survival for >8 weeks of lamina propria (LP) CD4(+) T cells from colitic SOD mice previously injected with CD4(+)CD45RB(high) T cells. These cells were in a quiescent state and divided a maximum of 5 times in 4 weeks. LP CD4(+) T cells expressed higher levels of Bcl-2, integrin-alpha 4 beta 7, CXCR3 and CD25 after than before culture, as well as secreting high concentrations of IL-2 and low concentrations of IFN-gamma and IL-17 in response to intestinal bacterial antigens. LP CD4(+) T cells from colitic mice cultured with IL-7 for 8 weeks induced more severe colitis than LP CD4(+) T cells cultured for 4 weeks. We developed a novel culture system to purify a long-lived, highly pathogenic memory subset from activated LP CD4(+) T cells. IL-7 promoted long-term in vitro survival of this subset in a quiescent state. This subset will be a novel, effective target for the treatment of IBD. en-copyright= kn-copyright= en-aut-name=TakaharaMasahiro en-aut-sei=Takahara en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NemotoYasuhiro en-aut-sei=Nemoto en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OshimaShigeru en-aut-sei=Oshima en-aut-mei=Shigeru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MatsuzawaYu en-aut-sei=Matsuzawa en-aut-mei=Yu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KanaiTakanori en-aut-sei=Kanai en-aut-mei=Takanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OkamotoRyuichi en-aut-sei=Okamoto en-aut-mei=Ryuichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TsuchiyaKiichiro en-aut-sei=Tsuchiya en-aut-mei=Kiichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=NakamuraTetsuya en-aut-sei=Nakamura en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=YamamotoKazuhide en-aut-sei=Yamamoto en-aut-mei=Kazuhide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=WatanabeMamoru en-aut-sei=Watanabe en-aut-mei=Mamoru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch affil-num=2 en-affil= kn-affil=Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch affil-num=3 en-affil= kn-affil=Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch affil-num=4 en-affil= kn-affil=Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch affil-num=5 en-affil= kn-affil=Keio Univ, Dept Internal Med, Div Gastroenterol & Hepatol, Sch Med affil-num=6 en-affil= kn-affil=Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch affil-num=7 en-affil= kn-affil=Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch affil-num=8 en-affil= kn-affil=Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch affil-num=9 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Gastroenterol & Hepatol affil-num=10 en-affil= kn-affil=Tokyo Med & Dent Univ, Dept Gastroenterol & Hepatol, Grad Sch en-keyword=Inflammatory bowel disease kn-keyword=Inflammatory bowel disease en-keyword=Memory T cells kn-keyword=Memory T cells en-keyword=CD4 kn-keyword=CD4 en-keyword=IL-7 kn-keyword=IL-7 en-keyword=Cell culture kn-keyword=Cell culture END start-ver=1.4 cd-journal=joma no-vol=1440 cd-vols= no-issue= article-no= start-page=145 end-page=149 dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20160401 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Direct determination of lignin peroxidase released from Phanerochaete chrysosporium by in-capillary enzyme assay using micellar electrokinetic chromatography en-subtitle= kn-subtitle= en-abstract= kn-abstract=Here we describe the application of an in-capillary enzyme assay using micellar electrokinetic chromatography (MEKC) in the determination of enzyme activity in a crude culture medium containing lignin peroxidase released from Phanerochaete chrysosporium (P. chrysosporium). The method consists of a plug?plug reaction between lignin peroxidase and its substrate, veratryl alcohol, the separation of the product, veratraldehyde, from the other components including the enzyme and the culture medium, and the determination of the enzyme activity from the peak area of veratraldehyde produced by the plug?plug reaction. This method is more sensitive than conventional spectrophotometry since the background originates from the enzyme and the culture medium can be removed via MEKC separation. Veratraldehyde was separated at ?10 kV in a background electrolyte containing 50 mM tartrate buffer (pH 2.5) and 50 mM sodium dodecyl sulfate (SDS) after a plug?plug reaction in the capillary for 5 min. The calibration curve of veratraldehyde was linear up to 4 pmol (500 μM) with a limit to quantification of 0.026 pmol (3.2 μM) (SN = 10). The activity of lignin peroxidase was directly measured from the peak area of veratraldehyde. The activity of lignin peroxidase released from P. chrysosporium into the medium for 7 days was successfully determined to be 3.40 UL?1. en-copyright= kn-copyright= en-aut-name=HaradaAiri en-aut-sei=Harada en-aut-mei=Airi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SasakiKeiko en-aut-sei=Sasaki en-aut-mei=Keiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KanetaTakashi en-aut-sei=Kaneta en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Department of Earth Resources Engineering, Graduate School of Engineering, Kyushu University affil-num=3 en-affil= kn-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University en-keyword=Lignin Peroxidase kn-keyword=Lignin Peroxidase en-keyword=Phanerochaete chrysosporium kn-keyword=Phanerochaete chrysosporium en-keyword=Micellar electrokinetic chromatography kn-keyword=Micellar electrokinetic chromatography en-keyword=Capillary electrophoresis kn-keyword=Capillary electrophoresis en-keyword=Enzyme assay kn-keyword=Enzyme assay END