このエントリーをはてなブックマークに追加
ID 50635
フルテキストURL
著者
Mizote, Yu Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Immunol
Taniguchi, Taku Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Immunol
Tanaka, Kei Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Immunol
Isobe, Midori Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Immunol
Wada, Hisashi Osaka Univ, Grad Sch Med, Dept Surg, Suita
Saika, Takashi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Urol
Kita, Shoichi Med & Biol Labs Ltd
Koide, Yukari Med & Biol Labs Ltd
Uenaka, Akiko Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Immunol
Nakayama, Eiichi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Immunol
抄録
Three novel NY-ESO-1 CD4 T cell epitopes were identified using PBMC obtained from patients who were vaccinated with a complex of cholesterol-bearing hydrophobized pullulan (CHP) and NY-ESO-1 protein (CHP-NY-ESO-1). The restriction molecules were determined by antibody blocking and using various EBV-B cells with different HLA alleles as APC to present peptides to CD4 T cells. The minimal epitope peptides were determined using various N- and C-termini truncated peptides deduced from 18-mer overlapping peptides originally identified for recognition. Those epitopes were DRB1*0901-restricted NY-ESO-1 87-100. DQB1*0401-restricted NY-ESO-1 95-107 and DRB1*0803-restricted NY-ESO-1 124-134. CD4 T cells used to determine those epitope peptides recognized EBV-B cells or DC that were treated with recombinant NY-ESO-1 protein or NY-ESO-1-expressing tumor cell lysate, suggesting that the epitope peptides are naturally processed. These CD4 T cells showed a cytokine profile with Th1 characteristics. Furthermore, NY-ESO-1 87-100 peptide/HLA-DRB1*0901 tetramer staining was observed. Multiple Th1-type CD4 T cell responses are beneficial for inducing effective anti-tumor responses after NY-ESO-1 protein vaccination.
発行日
2010-07-19
出版物タイトル
Vaccine
28巻
32号
出版者
Elsevier Sci Ltd
開始ページ
5338
終了ページ
5346
ISSN
0264-410X
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1016/j.vaccine.2010.05.044
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/50954
言語
English
OAI-PMH Set
岡山大学
著作権者
(C) 2010 Elsevier Ltd. All rights reserved.
論文のバージョン
author
査読
有り
DOI
Web of Sience KeyUT