ID | 32969 |
フルテキストURL | |
著者 |
Amin, Md. Ziaul
Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Bando, Tetsuya
Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Ruksana, Razia
Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Anokye-Danso, Frederick
Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Takashima, Yasuo
Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Sakube, Yasuji
Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Kagawa, Hiroaki
Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
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抄録 | The aim of this study is to investigate the function of the C-terminal extension of three troponin I isoforms, that are unique to the body wall muscles of Caenorhabditis elegans and to understand the molecular interactions within the TN complex between troponin I with troponin C/T, and tropomyosin. We constructed several expression vectors to generate recombinant proteins of three body wall and one pharyngeal troponin I isoforms in Escherichia coli. Protein overlay assays and Western blot analyses were performed using antibodies. We demonstrated that pharyngeal TNI-4 interacted with only the pharyngeal isoforms of troponin C/T and tropomyosin. In contrast, the body wall TNI-2 bound both the body wall and pharyngeal isoforms of these components. Similar to other invertebrates, the N-terminus of troponin I contributes to interactions with troponin C. Full-length troponin I was essential for interactions with tropomyosin isoforms. Deletion of the C-terminal extension had no direct effect on the binding of the body wall troponin I to other muscle thin filament troponin C/T and tropomyosin isoforms.
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キーワード | Troponin I
Caenorhabditis elegans
Troponin T
Troponin C
Tropomyosin
C-terminal extension
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備考 | Published with permission from the copyright holder. This is a author's copy,as published in Biochimica Biophysica et Acta, April 2007, volume 1774, issue 4, pp456-465.
Publisher URL: http://dx.doi.org/10.1016/j.bbapap.2007.01.003 Copyright © 2007 Elsevier B. V. All rights reserved. |
発行日 | 2007-04
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出版物タイトル |
Biochimica et Biophysica Acta. Proteins and Proteomics
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巻 | 1774巻
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号 | 4号
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出版者 | Elsevier B.V.
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開始ページ | 456
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終了ページ | 465
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ISSN | 1570-9639
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NCID | AA11685085
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資料タイプ |
学術雑誌論文
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言語 |
英語
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著作権者 | Elsevier B.V.
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論文のバージョン | author
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査読 |
有り
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DOI | |
PubMed ID | |
Web of Science KeyUT | |
Submission Path | biology_general/35
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