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ID 32969
フルテキストURL
著者
Amin, Md. Ziaul Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Bando, Tetsuya Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Ruksana, Razia Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Anokye-Danso, Frederick Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Takashima, Yasuo Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Sakube, Yasuji Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Kagawa, Hiroaki Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
抄録
The aim of this study is to investigate the function of the C-terminal extension of three troponin I isoforms, that are unique to the body wall muscles of Caenorhabditis elegans and to understand the molecular interactions within the TN complex between troponin I with troponin C/T, and tropomyosin. We constructed several expression vectors to generate recombinant proteins of three body wall and one pharyngeal troponin I isoforms in Escherichia coli. Protein overlay assays and Western blot analyses were performed using antibodies. We demonstrated that pharyngeal TNI-4 interacted with only the pharyngeal isoforms of troponin C/T and tropomyosin. In contrast, the body wall TNI-2 bound both the body wall and pharyngeal isoforms of these components. Similar to other invertebrates, the N-terminus of troponin I contributes to interactions with troponin C. Full-length troponin I was essential for interactions with tropomyosin isoforms. Deletion of the C-terminal extension had no direct effect on the binding of the body wall troponin I to other muscle thin filament troponin C/T and tropomyosin isoforms.
キーワード
Troponin I
Caenorhabditis elegans
Troponin T
Troponin C
Tropomyosin
C-terminal extension
備考
Published with permission from the copyright holder. This is a author's copy,as published in Biochimica Biophysica et Acta, April 2007, volume 1774, issue 4, pp456-465.
Publisher URL: http://dx.doi.org/10.1016/j.bbapap.2007.01.003
Copyright © 2007 Elsevier B. V. All rights reserved.
発行日
2007-04
出版物タイトル
Biochimica et Biophysica Acta. Proteins and Proteomics
1774巻
4号
出版者
Elsevier B.V.
開始ページ
456
終了ページ
465
ISSN
1570-9639
NCID
AA11685085
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
Elsevier B.V.
論文のバージョン
author
査読
有り
DOI
PubMed ID
Web of Sience KeyUT
Submission Path
biology_general/35