start-ver=1.4 cd-journal=joma no-vol=27 cd-vols= no-issue=1 article-no= start-page=181 end-page=189 dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210210 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Medial meniscus posterior root repairs: A comparison among three surgical techniques in short-term clinical outcomes and arthroscopic meniscal healing scores en-subtitle= kn-subtitle= en-abstract= kn-abstract=Background
Medial meniscus (MM) posterior root repairs lead to favorable clinical outcomes in patients with MM posterior root tears (MMPRTs). However, there are few comparative studies in evaluating the superiority among several pullout repair techniques such as modified Mason?Allen suture, simple stitch, and concomitant posteromedial pullout repair. We hypothesized that an additional pullout suture at the MM posteromedial part would have clinical advantages in transtibial pullout repairs of the MMPRTs. The aim of this study was to compare the clinical usefulness among several types of pullout repair techniques in patients with MMPRTs.
Methods
Eighty-three patients who underwent arthroscopic pullout repairs of the MMPRTs were investigated. Patients were divided into three groups using different pullout repair techniques: a modified Mason?Allen suture using FasT-Fix all-inside meniscal repair device (F-MMA, n?=?28), two simple stitches (TSS, n?=?30), and TSS concomitant with posteromedial pullout repair using all-inside meniscal repair device (TSS-PM, n?=?25). Postoperative clinical outcomes and semi-quantitative arthroscopic meniscal healing scores (0?10 points) were evaluated at second-look arthroscopies.
Results
No significant differences among the three groups were observed in patient demographics and preoperative clinical scores, except for preoperative Lysholm scores. At second-look arthroscopies, there were no significant differences among the three techniques in postoperative clinical outcomes and meniscal healing scores.
Conclusions
This study demonstrated that the TSS-PM pullout repair technique did not show better scores in postoperative clinical outcomes and meniscal healings compared with the F-MMA and TSS techniques. Our results suggest that the concomitant posteromedial pullout suture may have no clinical advantage in the conventional pullout repairs for the patients with MMPRTs. en-copyright= kn-copyright= en-aut-name=FurumatsuTakayuki en-aut-sei=Furumatsu en-aut-mei=Takayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HiranakaTakaaki en-aut-sei=Hiranaka en-aut-mei=Takaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OkazakiYuki en-aut-sei=Okazaki en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KintakaKeisuke en-aut-sei=Kintaka en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KodamaYuya en-aut-sei=Kodama en-aut-mei=Yuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KamatsukiYusuke en-aut-sei=Kamatsuki en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OzakiToshifumi en-aut-sei=Ozaki en-aut-mei=Toshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Department of Orthopaedic Surgery, Okayama University Hospital kn-affil= affil-num=2 en-affil=Department of Orthopaedic Surgery, Okayama University Hospital kn-affil= affil-num=3 en-affil=Department of Orthopaedic Surgery, Okayama University Hospital kn-affil= affil-num=4 en-affil=Department of Orthopaedic Surgery, Okayama University Hospital kn-affil= affil-num=5 en-affil=Department of Orthopaedic Surgery, Okayama University Hospital kn-affil= affil-num=6 en-affil=Department of Orthopaedic Surgery, Okayama University Hospital kn-affil= affil-num=7 en-affil=Department of Orthopaedic Surgery, Okayama University Hospital kn-affil= END start-ver=1.4 cd-journal=joma no-vol=8 cd-vols= no-issue= article-no= start-page=41 end-page=44 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20190731 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A proposed simple screening method to determine relative contributions of CYP3A4 and CYP3A5 to drug metabolism in?vitro en-subtitle= kn-subtitle= en-abstract= kn-abstract=Purpose
The cytochrome P450 (CYP) 3A family of enzymes metabolize the majority of clinically used drugs. CYP3A4 and CYP3A5 are the two major CYP3A isoforms, but exhinbit different substrate specificity. The aim of this study was to establish a simple screening method to determine the relative contributions of CYP3A4 and CYP3A5 to drug metabolism in vitro.
Methods
A screening method was developed based on competitive inhibition using luciferin-PPXE (L-PPXE), a luminogenic CYP3A substrate. CYP3cide, tacrolimus, and midazolam were selected as standard compounds metabolized by CYP3A4 or CYP3A5. Nine clinically-used drugs were evaluated for their abilities to inhibit luminescence resulting from L-PPXE metabolism. Appropriate reaction conditions for the screening method were determined using recombinant CYP3A4 and CYP3A5.
Results
A significant decrease in luminescence resulting from L-PPXE metabolism by CYP3A4 and CYP3A5 was observed only for drugs reported to be metabolized by CYP3As. The substrate specificities of CYP3A4 or CYP3A5 for the proposed CYP3A substrates using our screening method were consistent with those of previous reports or available drug information from pharmaceutical companies. The reaction volume for this method was 50 ƒÊL, and the time required for the entire procedure was 70 min. Furthermore, this screening can be performed using a single tube with minimal training.
Conclusions
Through the establishment of our screening method in the present study, we are sure it is useful to determine the relative contributions of CYP3A4 and CYP3A5 to drug metabolism in vitro. en-copyright= kn-copyright= en-aut-name=MatsumotoJun en-aut-sei=Matsumoto en-aut-mei=Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakamuraHiroyoshi en-aut-sei=Nakamura en-aut-mei=Hiroyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SanSu Nwe en-aut-sei=San en-aut-mei=Su Nwe kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SatoHikari en-aut-sei=Sato en-aut-mei=Hikari kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TakezawaManami en-aut-sei=Takezawa en-aut-mei=Manami kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KishiRyuto en-aut-sei=Kishi en-aut-mei=Ryuto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KitoYutaro en-aut-sei=Kito en-aut-mei=Yutaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=SuganoJunko en-aut-sei=Sugano en-aut-mei=Junko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=IzukiMai en-aut-sei=Izuki en-aut-mei=Mai kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=YanagisawaNao en-aut-sei=Yanagisawa en-aut-mei=Nao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=IkedaNaoki en-aut-sei=Ikeda en-aut-mei=Naoki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=SaitoYusuke en-aut-sei=Saito en-aut-mei=Yusuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=KatoYoshinori en-aut-sei=Kato en-aut-mei=Yoshinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=YamadaHarumi en-aut-sei=Yamada en-aut-mei=Harumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=FujiyoshiMasachika en-aut-sei=Fujiyoshi en-aut-mei=Masachika kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= en-aut-name=AriyoshiNoritaka en-aut-sei=Ariyoshi en-aut-mei=Noritaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=16 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=2 en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare kn-affil= affil-num=3 en-affil=Graduate School of Pharmaceutical Sciences, International University of Health and Welfare kn-affil= affil-num=4 en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare kn-affil= affil-num=5 en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare kn-affil= affil-num=6 en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare kn-affil= affil-num=7 en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare kn-affil= affil-num=8 en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare kn-affil= affil-num=9 en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare kn-affil= affil-num=10 en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare kn-affil= affil-num=11 en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare kn-affil= affil-num=12 en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare kn-affil= affil-num=13 en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare kn-affil= affil-num=14 en-affil=Department of Pharmaceutical Sciences, International University of Health and Welfare kn-affil= affil-num=15 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=16 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= en-keyword=CYP3A kn-keyword=CYP3A en-keyword=CYP3A5*3 allele kn-keyword=CYP3A5*3 allele en-keyword=P450 Glo Assay system kn-keyword=P450 Glo Assay system en-keyword=Pharmacokinetics kn-keyword=Pharmacokinetics en-keyword=Pharmacogenetics kn-keyword=Pharmacogenetics END start-ver=1.4 cd-journal=joma no-vol=924 cd-vols= no-issue= article-no= start-page=60 end-page=67 dt-received= dt-revised= dt-accepted= dt-pub-year=2016 dt-pub=20160614 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Chelate titrations of Ca2+ and Mg2+ using microfluidic paper-based analytical devices en-subtitle= kn-subtitle= en-abstract= kn-abstract=We developed microfluidic paper-based analytical devices (ƒÊPADs) for the chelate titrations of Ca2+ and Mg2+ in natural water. The ƒÊPAD consisted of ten reaction zones and ten detection zones connected through narrow channels to a sample zone located at the center. Buffer solutions with a pH of 10 or 13 were applied to all surfaces of the channels and zones. Different amounts of ethylenediaminetetraacetic acid (EDTA) were added to the reaction zones and a consistent amount of a metal indicator (Eriochrome Black T or Calcon) was added to the detection zones. The total concentrations of Ca2+ and Mg2+ (total hardness) in the water were measured using a ƒÊPAD containing a buffer solution with a pH of 10, whereas only Ca2+ was titrated using a ƒÊPAD prepared with a potassium hydroxide solution with a pH of 13. The ƒÊPADs permitted the determination of Ca2+ and Mg2+ in mineral water, river water, and seawater samples within only a few minutes using only the naked eye?no need of instruments. en-copyright= kn-copyright= en-aut-name=KaritaShingo en-aut-sei=Karita en-aut-mei=Shingo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KanetaTakashi en-aut-sei=Kaneta en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University kn-affil=‰ªŽR‘åŠw‘åŠw‰@Ž©‘R‰ÈŠwŒ¤‹†‰È affil-num=2 en-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University kn-affil=‰ªŽR‘åŠw‘åŠw‰@Ž©‘R‰ÈŠwŒ¤‹†‰È en-keyword=Chelate titration kn-keyword=Chelate titration en-keyword=Microfluidic paper-based analytical device kn-keyword=Microfluidic paper-based analytical device en-keyword=Hardness kn-keyword=Hardness en-keyword=Calcium kn-keyword=Calcium en-keyword=Magnesium kn-keyword=Magnesium en-keyword=Natural water kn-keyword=Natural water END