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ID 48823
フルテキストURL
著者
Koreishi, Mayuko The Research Core for Interdisciplinary Sciences (RCIS), Okayama University
Honjo, Yasuko The Research Core for Interdisciplinary Sciences (RCIS), Okayama University
Satoh, Ayano The Research Core for Interdisciplinary Sciences (RCIS), Okayama University ORCID Kaken ID publons
抄録
Gene perturbation methods are commonly used in the study of gene and protein function. The authors of this paper recently developed a rapid protein inactivation technique utilizing tobacco etch virus (TEV)-derived protease. TEV protease recognizes the ENLYFQG (Glu-Asn-Leu-Tyr-Phe-Gln-Gly) amino acid sequence and specifically cleaves between Q and G. The authors developed antibodies that recognize the cleaved TEV (ENLYFQ) sequence, both in vitro and in vivo, but do not bind to uncleaved TEV (ENLYFQG). Using these antibodies, in situ protein cleavage was successfully detected. These antibodies used in combination with the TEV protease may be a useful complement to other perturbation methods.
キーワード
TEV protease
Golgi
golgins
microinjection
recombinant proteins
発行日
201-08-09
出版物タイトル
Antibody Technology Journal
2011巻
1号
出版者
Dove Medical Press
開始ページ
5
終了ページ
11
ISSN
2230-3170
資料タイプ
学術雑誌論文
オフィシャル URL
http://www.dovepress.com/detection-of-in-situ-cleaved-p115-with-the-cut-specific-antibodies-in--peer-reviewed-article-ANTI
言語
English
著作権者
© 2011 Koreishi et al
論文のバージョン
publisher
査読
有り
DOI