Koreishi, Mayuko The Research Core for Interdisciplinary Sciences (RCIS), Okayama University
Honjo, Yasuko The Research Core for Interdisciplinary Sciences (RCIS), Okayama University
Gene perturbation methods are commonly used in the study of gene and protein function. The authors of this paper recently developed a rapid protein inactivation technique utilizing tobacco etch virus (TEV)-derived protease. TEV protease recognizes the ENLYFQG (Glu-Asn-Leu-Tyr-Phe-Gln-Gly) amino acid sequence and specifically cleaves between Q and G. The authors developed antibodies that recognize the cleaved TEV (ENLYFQ) sequence, both in vitro and in vivo, but do not bind to uncleaved TEV (ENLYFQG). Using these antibodies, in situ protein cleavage was successfully detected. These antibodies used in combination with the TEV protease may be a useful complement to other perturbation methods.
Antibody Technology Journal
Dove Medical Press
© 2011 Koreishi et al