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ID 60535
フルテキストURL
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著者
Kohara, Keigo Department of Cellular and Functional Biology, Institute of Biomedical Science, Kansai Medical University
Inoue, Akitoshi Department of Medical Chemistry, Kansai Medical University, Graduate School of Medicine
Nakano, Yousuke Department of Anatomy, Kansai Medical University, Graduate School of Medicine
Hirai, Hirokazu Department of Neurophysiology and Neural Repair, Gunma University Graduate School of Medicine
Kobayashi, Takuya Department of Medical Chemistry, Kansai Medical University, Graduate School of Medicine, Hirakata
Maruyama, Masato Faculty of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
Baba, Ryosuke Department of Cellular and Functional Biology, Institute of Biomedical Science, Kansai Medical University
Kawashima, Chiho Department of Cellular and Functional Biology, Institute of Biomedical Science, Kansai Medical University
抄録
Elucidating fine architectures and functions of cellular and synaptic connections requires development of new flexible methods. Here, we created a concept called the “battle of transgenes,” based on which we generated strategies using genetically engineered battles of multiple recombinases. The strategies enabled split-tunable allocation of multiple transgenes. We demonstrated the versatility of these strategies and technologies in inducing strong and multi-sparse allocations of multiple transgenes. Furthermore, the combination of our transgenic strategy and expansion microscopy enabled three-dimensional high-resolution imaging of whole synaptic structures in the hippocampus with simultaneous visualizations of endogenous synaptic proteins. These strategies and technologies based on the battle of genes may accelerate the analysis of whole synaptic and cellular connections in diverse life science fields.
発行日
2020-06-26
出版物タイトル
iScience
23巻
6号
出版者
Cell Press
開始ページ
101248
ISSN
25890042
資料タイプ
学術雑誌論文
言語
英語
OAI-PMH Set
岡山大学
著作権者
© 2020 The Author(s).
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publisher
DOI
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ライセンス
https://creativecommons.org/licenses/by-nc-nd/4.0/
オープンアクセス(出版社)
OA
オープンアーカイブ(出版社)
非OpenArchive