このエントリーをはてなブックマークに追加
ID 55412
フルテキストURL
Thumnail Figure 2.TIF 90.6 KB
Thumnail Table 1.TIF 186 KB
Thumnail fig_all.pdf 215 KB
著者
Kuse, Mariko Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
Sakumoto, Ryosuke Reproductive Biology Research Unit, National Institute of Agrobiological Sciences
Okuda, Kiyoshi Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University
抄録
Progesterone (P4) acts through different actuating pathways called genomic and non-genomic pathways. Here we investigated whether P4 regulates prostaglandin (PG) F2? (PGF) and PGE2 production in bovine endometrium through different pathways. Cultured endometrial cells were exposed to P4 for a short time (5-20min) or bovine serum albumin (BSA)-conjugated P4 (P4-BSA) for 24h. Progesterone treatment for 24h stimulated PGE2 production in epithelial cells, but suppressed both PGF and PGE2 production and the expression of PG-metabolising enzymes including phospholipase A2 (PLA2) and cyclooxygenase-2 (COX2) in stromal cells. Short-term (5-20min) P4 treatment did not affect PLA2 or COX2 transcript levels in either cell type. P4-BSA increased PGF and PGE2 production only in epithelial cells. Nuclear P4 receptor mRNA expression in endometrium was higher at the follicular phase than at the early- to mid-luteal stages, whereas membrane P4 receptor mRNA expression did not change throughout the oestrous cycle. The overall results suggest that P4 controls PG production by inhibiting enzymes via a genomic pathway and by stimulating signal transduction via a non-genomic pathway. Consequently, P4 may protect the corpus luteum by attenuating PGF production in stromal cells and by increasing PGE2 secretion from epithelial cells
キーワード
cows
luteolysis
steroid hormone
uterus
備考
This is an Accepted Manuscript of an article published by CSIRO Publishing.
発行日
2015-04
出版物タイトル
Reproduction, Fertility and Development
28巻
10号
出版者
CSIRO Publishing
開始ページ
1588
終了ページ
1597
ISSN
1031-3613
NCID
AA10718189
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン
author
PubMed ID
DOI
Web of Sience KeyUT
関連URL
isVersionOf https://doi.org/10.1071/RD14490