start-ver=1.4 cd-journal=joma no-vol=39 cd-vols= no-issue=3 article-no= start-page=188 end-page=196 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=2020 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Cyclic nigerosylnigerose ameliorates DSS-induced colitis with restoration of goblet cell number and increase in IgA reactivity against gut microbiota in mice en-subtitle= kn-subtitle= en-abstract= kn-abstract=Cyclic nigerosylnigerose (CNN) is a cyclic oligosaccharide. Oral administration of CNN promotes immunoglobulin A (IgA) secretion in the gut. IgA is a major antibody secreted into the gut and plays a crucial role in suppressing gut inflammation due to commensal gut microbiota. To investigate the effect of administration of CNN to promote IgA secretion on gut inflammation, experimental colitis was induced with dextran sulfate sodium (DSS) in Balb/c mice after 6 weeks of CNN pre-feeding. The severity of colitis was evaluated based on a disease activity index (DAI), the gene expression of inflammatory cytokines, and a histological examination. The CNN-treated mice with DSS-induced colitis (CNN-DSS group) showed significantly lower DAI scores and mRNA levels of interleukin-1 compared with the CNN-untreated mice with DSS-induced colitis (DSS group). Histological examination of the colon revealed that the pathological score was significantly lower in the CNN-DSS group compared with the DSS group due to the reduced infiltration of immune cells. The number of goblet cells was significantly higher in the CNN-DSS group compared with the DSS group. The IgA concentration and the ratio of microbiota coated with IgA were evaluated in the cecal content. Although there was no difference in the IgA concentration among groups, a higher proportion of cecal microbiota were coated with IgA in the CNN-DSS group compared with that in the DSS group. These results suggest that CNN might preserve goblet cells in the colon and promote IgA coating of gut microbiota, which synergistically ameliorate gut inflammation in mice with DSS-induced colitis. en-copyright= kn-copyright= en-aut-name=TsurutaTakeshi en-aut-sei=Tsuruta en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KatsumataEmiko en-aut-sei=Katsumata en-aut-mei=Emiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MizoteAkiko en-aut-sei=Mizote en-aut-mei=Akiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=JianHou Jian en-aut-sei=Jian en-aut-mei=Hou Jian kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MuhomahTeresia Aluoch en-aut-sei=Muhomah en-aut-mei=Teresia Aluoch kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NishinoNaoki en-aut-sei=Nishino en-aut-mei=Naoki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=2 en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=3 en-affil=Hayashibara Co., Ltd. kn-affil= affil-num=4 en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=5 en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=6 en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University kn-affil= en-keyword=oligosaccharide kn-keyword=oligosaccharide en-keyword=DSS-induced colitis kn-keyword=DSS-induced colitis en-keyword=goblet cell kn-keyword=goblet cell en-keyword=gut microbiota kn-keyword=gut microbiota en-keyword=immunoglobulin A kn-keyword=immunoglobulin A END start-ver=1.4 cd-journal=joma no-vol=39 cd-vols= no-issue=3 article-no= start-page=117 end-page=122 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200227 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Oral administration of the probiotic bacterium Lactobacillus acidophilus strain L-55 modulates the immunological parameters of the laying hen inoculated with a Newcastle disease virus-based live attenuated vaccine en-subtitle= kn-subtitle= en-abstract= kn-abstract=Probiotic supplements containing living bacteria have attracted interest as a potential source of health benefits for humans and livestock. The aim of this study was to determine whether administration of Lactobacillus acidophilus strain L-55 (LaL-55) enhances the immune response among chicks exposed to a Newcastle disease virus (NDV)-based live attenuated vaccine. Oral administration of LaL-55 augmented the elevation in the total numbers of leukocytes and lymphocytes following inoculation with the NDV-based live attenuated vaccine. Monocyte counts increased after LaL-55 administration independent of inoculation with the NDV vaccine. Among chicks that were administered LaL-55, there was a dose-dependent increase in the NK cell activity measured by a 51Cr release assay at 2 weeks after the secondary NDV vaccine inoculation. Two weeks after the secondary inoculation with the NDV vaccine, interferon (IFN)-ƒÁ-mRNA expression was significantly elevated in mononuclear splenocytes from chicks that were administered LaL-55. Meanwhile, LaL-55 administration did not change the mRNA levels of IFN-ƒ¿, IFN-ƒÀ, and interleukin-1ƒÀ. These results may suggest that coadministration of LaL-55 with an NDV vaccine augments the immune response against the virus. Therefore, LaL-55 may help protect against viral diseases in poultry. en-copyright= kn-copyright= en-aut-name=HoDung Thi en-aut-sei=Ho en-aut-mei=Dung Thi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HatabuToshimitsu en-aut-sei=Hatabu en-aut-mei=Toshimitsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SunadaYosuke en-aut-sei=Sunada en-aut-mei=Yosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KondoYasuhiro en-aut-sei=Kondo en-aut-mei=Yasuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=2 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=3 en-affil=Research & Development, Ohayo Dairy Products Co., Ltd. kn-affil= affil-num=4 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= en-keyword=Lactobacillus acidophilus L-55 kn-keyword=Lactobacillus acidophilus L-55 en-keyword=chicken kn-keyword=chicken en-keyword=Newcastle disease vaccine kn-keyword=Newcastle disease vaccine en-keyword=immunomodulatory kn-keyword=immunomodulatory END