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ID 48122
フルテキストURL
著者
Ono, Takehiro Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Tsuji, Tomoe Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Sakai, Miho Miyazaki Prefectural Food R&D Center
Yukizaki, Chizuko Miyazaki Prefectural Food R&D Center
Ino, Hisatoshi Miyazaki Agricultural Research Institute
Akagi, Isao Miyazaki Prefectural Industrial Support Foundation
Hiramatsu, Kaori Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Matsumoto, Yohsuke Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Sugiura, Yoshihiro Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Uto, Hirofumi Department of Digestive Disease and Life-style Related Diseases, Health Research Human and Environmental Sciences, Kagoshima University Graduate School of Medical and Dental Sciences
Tsubouchi, Hirohito Department of Digestive Disease and Life-style Related Diseases, Health Research Human and Environmental Sciences, Kagoshima University Graduate School of Medical and Dental Sciences
Gohda, Eiichi Department of Immunochemistry, Division of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
抄録
Hepatocyte growth factor (HGF) is useful as a potential therapeutic agent for hepatic and renal fibrosis and cardiovascular diseases through inducing proliferation of epithelial and endothelial cells. HGF inducers may also be useful as therapeutic agents for these diseases. However, there have been no reports on induction of HGF production by plant extracts or juices. An extract of bitter melon (Momordica charantia L.) pulp markedly induced HGF production. There was a time lag of 72 h before induction of HGF production after the extract addition. Its stimulatory effect was accompanied by upregulation of HGF gene expression. Increases in mitogen-activated protein kinases (MAPKs) were observed from 72 h after the extract addition. Inhibitors of MAPKs suppressed the extract-induced HGF production. The extract also stimulated cell proliferation. Both activities for induction of HGF production and cell proliferation were eluted together in a single peak with 14,000 Da on gel filtration. The results indicate that bitter melon pulp extract induced HGF production and cell proliferation of human dermal fibroblasts and suggest that activation of MAPKs is involved in the HGF induction. Our findings suggest potential usefulness of the extract for tissue regeneration and provide an insight into the molecular mechanism underlying the wound-healing property of bitter melon.
キーワード
Hepatocyte growth factor
Bitter melon
Extracellular signal-regulated kinase
Cell proliferation
Dermal fibroblast
発行日
2009-04
出版物タイトル
Cytokine
46巻
1号
出版者
Academic Press Ltd. Elsevier Science Ltd.
開始ページ
119
終了ページ
126
ISSN
1043-4666
NCID
AA10794201
資料タイプ
学術雑誌論文
言語
English
著作権者
© 2009 Elsevier Ltd. All rights reserved.
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