ID | 50636 |
フルテキストURL | |
著者 |
Kuroki, Misao
Okayama Univ, Dept Tumor Virol, Sch Med Dent & Pharmaceut Sci
Ariumi, Yasuo
Okayama Univ, Dept Tumor Virol, Sch Med Dent & Pharmaceut Sci
Hijikata, Makoto
Kyoto Univ, Dept Viral Oncol, Inst Virus Res
Ikeda, Masanori
Okayama Univ, Dept Tumor Virol, Sch Med Dent & Pharmaceut Sci
Kaken ID
publons
researchmap
Dansako, Hiromichi
Okayama Univ, Dept Tumor Virol, Sch Med Dent & Pharmaceut Sci
ORCID
Kaken ID
publons
researchmap
Wakita, Takaji
Natl Inst Infect Dis, Dept Virol 2
Shimotohno, Kunitada
Natl Ctr Global Hlth & Med, Res Ctr Hepatitis & Immunol
Kato, Nobuyuki
Okayama Univ, Dept Tumor Virol, Sch Med Dent & Pharmaceut Sci
Kaken ID
publons
researchmap
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抄録 | PML tumor suppressor protein, which forms discrete nuclear structures termed PML-nuclear bodies, has been associated with several cellular functions, including cell proliferation, apoptosis and antiviral defense. Recently, it was reported that the HCV core protein colocalizes with PML in PML-NBs and abrogates the PML function through interaction with PML. However, role(s) of PML in HCV life cycle is unknown. To test whether or not PML affects HCV life cycle, we examined the level of secreted HCV core and the infectivity of HCV in the culture supernatants as well as the level of HCV RNA in HuH-7-derived RSc cells, in which HCV-JFH1 can infect and efficiently replicate, stably expressing short hairpin RNA targeted to PML. In this context, the level of secreted HCV core and the infectivity in the supernatants from PML knockdown cells was remarkably reduced, whereas the level of HCV RNA in the PML knockdown cells was not significantly affected in spite of very effective knockdown of PML. In fact, we showed that PML is unrelated to HCV RNA replication using the subgenomic HCV-JFH1 replicon RNA, JRN/3-5B. Furthermore, the infectivity of HCV-like particle in the culture supernatants was significantly reduced in PML knockdown JRN/3-5B cells expressing core to NS2 coding region of HCV-JFH1 genome using the trans-packaging system. Finally, we also demonstrated that INI1 and DDX5, the PML-related proteins, are involved in HCV production. Taken together, these findings suggest that PML is required for HCV production.
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キーワード | Hepatitis C virus
PML
INI1
DDX5
Tumor suppressor
Lipid droplet
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発行日 | 2013-01-11
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出版物タイトル |
Biochemical and Biophysical Research Communications
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巻 | 430巻
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号 | 2号
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出版者 | Academic Press Inc Elsevier Science
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開始ページ | 592
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終了ページ | 597
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ISSN | 0006-291X
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NCID | AA00564395
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資料タイプ |
学術雑誌論文
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オフィシャル URL | http://dx.doi.org/10.1016/j.bbrc.2012.11.108
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関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/50690
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言語 |
英語
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著作権者 | (C) 2012 Published by Elsevier Inc. All rights reserved.
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論文のバージョン | author
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査読 |
有り
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DOI | |
Web of Science KeyUT |