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ID 59926
フルテキストURL
著者
Moriya, Hisao Graduate School of Environmental and Life Sciences, Research Core for Interdisciplinary Sciences, Okayama University ORCID Kaken ID publons researchmap
抄録
Nucleotide and amino acid sequences at the N-terminus affect the expression level and cytotoxicity of proteins; however, their effects are not fully understood yet. Here, N-terminal 30 nucleotide/10 amino acid (N10) sequences that affect the expression level and cytotoxicity of a green fluorescent protein were systematically isolated in the budding yeast Saccharomyces cerevisiae. The expression per gene (EPG) and gene copy number limit (CNL) relationships were examined to assess the effects of the N10 sequence. The isolated N10 nucleotide sequences suggested that codon optimality is the major determinant of the protein expression level. A higher number of hydrophobic or cysteine residues in the N10 sequence seemed to increase the cytotoxicity of the protein. Therefore, a high frequency of specific amino acid residues in the outside of the main tertiary structure of proteins might not be preferable.
キーワード
green fluorescent protein
overexpression
expression limit
expression level
protein cytotoxicity
発行日
2020-05-14
出版物タイトル
AIMS Biophysics
7巻
2号
出版者
AIMS Press
開始ページ
121
終了ページ
132
ISSN
2377-9098
資料タイプ
学術雑誌論文
言語
英語
OAI-PMH Set
岡山大学
著作権者
© 2020 the Author(s), licensee AIMS Press.
論文のバージョン
publisher
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.3934/biophy.2020010
ライセンス
http://creativecommons.org/licenses/by/4.0