ID | 59926 |
フルテキストURL | |
著者 |
Moriya, Hisao
Graduate School of Environmental and Life Sciences, Research Core for Interdisciplinary Sciences, Okayama University
ORCID
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抄録 | Nucleotide and amino acid sequences at the N-terminus affect the expression level and cytotoxicity of proteins; however, their effects are not fully understood yet. Here, N-terminal 30 nucleotide/10 amino acid (N10) sequences that affect the expression level and cytotoxicity of a green fluorescent protein were systematically isolated in the budding yeast Saccharomyces cerevisiae. The expression per gene (EPG) and gene copy number limit (CNL) relationships were examined to assess the effects of the N10 sequence. The isolated N10 nucleotide sequences suggested that codon optimality is the major determinant of the protein expression level. A higher number of hydrophobic or cysteine residues in the N10 sequence seemed to increase the cytotoxicity of the protein. Therefore, a high frequency of specific amino acid residues in the outside of the main tertiary structure of proteins might not be preferable.
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キーワード | green fluorescent protein
overexpression
expression limit
expression level
protein cytotoxicity
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発行日 | 2020-05-14
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出版物タイトル |
AIMS Biophysics
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巻 | 7巻
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号 | 2号
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出版者 | AIMS Press
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開始ページ | 121
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終了ページ | 132
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ISSN | 2377-9098
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2020 the Author(s), licensee AIMS Press.
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論文のバージョン | publisher
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関連URL | isVersionOf https://doi.org/10.3934/biophy.2020010
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ライセンス | http://creativecommons.org/licenses/by/4.0
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