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ID 47455
フルテキストURL
著者
Umena, Yasufumi Department of Chemistry, Graduate School of Science, Osaka City University
Kawakami, Keisuke Division of Bioscience, Graduate School of Natural Science and Technology/Faculty of Science; Okayama University
Shen, Jian-Ren Division of Bioscience, Graduate School of Natural Science and Technology/Faculty of Science; Okayama University ORCID Kaken ID publons researchmap
Kamiya, Nobuo Department of Chemistry, Graduate School of Science, Osaka City University
抄録
Photosystem II is the site of photosynthetic water oxidation and contains 20 subunits with a total molecular mass of 350 kDa. The structure of photosystem II has been reported at resolutions from 3.8 to 2.9 angstrom. These resolutions have provided much information on the arrangement of protein subunits and cofactors but are insufficient to reveal the detailed structure of the catalytic centre of water splitting. Here we report the crystal structure of photosystem II at a resolution of 1.9 angstrom. From our electron density map, we located all of the metal atoms of the Mn(4)CaO(5) cluster, together with all of their ligands. We found that five oxygen atoms served as oxo bridges linking the five metal atoms, and that four water molecules were bound to the Mn(4)CaO(5) cluster; some of them may therefore serve as substrates for dioxygen formation. We identified more than 1,300 water molecules in each photosystem II monomer. Some of them formed extensive hydrogen-bonding networks that may serve as channels for protons, water or oxygen molecules. The determination of the high-resolution structure of photosystem II will allow us to analyse and understand its functions in great detail.
発行日
2011-05-05
出版物タイトル
Nature
473巻
7345号
出版者
Nature Publishing Group
開始ページ
55
終了ページ
60
ISSN
0028-0836
NCID
AA00752384
資料タイプ
学術雑誌論文
プロジェクト
エネルギー環境新素材拠点
言語
English
著作権者
©2011 Macmillan Publishers Limited. All rights reserved
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