ID | 50622 |
フルテキストURL | |
著者 |
Jiyou, Wang
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci
Katoh, Keizo
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci
Senoh, Mitsutoshi
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci
Mizuno, Tamaki
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci
researchmap
Maehara, Yoko
Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci
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抄録 | Vibrio vulnificus is a ubiquitous estuarine microorganism but causes fatal systemic infections in immunocompromised humans, cultured eels or shrimps. An extracellular metalloprotease VVP/VvpE has been reported to be a potential virulence factor of the bacterium; however, a few strains isolated from a diseased eel or shrimp were recently found to produce a serine protease termed VvsA, but not VVP/VvpE. In the present study, we found that these strains had lost the 80 kb genomic region including the gene encoding VVP/VvpE. We also purified VvsA from the culture supernatant through ammonium sulfate fractionation, gel filtration and ion-exchange column chromatography, and the enzyme was demonstrated to be a chymotrypsin-like protease, as well as those from some vibrios. The gene vvsA was shown to constitute an operon with a downstream gene vvsB, and several Vibrio species were found to have orthologues of vvsAB. These findings indicate that the genes vvp/vvpE and vvsAB might be mobile genetic elements.
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キーワード | Polymerase chain reaction
Purification
Serine protease
Metalloprotease
Vibrio vulnificus
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発行日 | 2012-04
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出版物タイトル |
World Journal of Microbiology & Biotechnology
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巻 | 28巻
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号 | 4号
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出版者 | Springer
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開始ページ | 1633
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終了ページ | 1639
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ISSN | 0959-3993
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資料タイプ |
学術雑誌論文
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プロジェクト |
インド感染症共同研究
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オフィシャル URL | http://dx.doi.org/10.1007/s11274-011-0969-y
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言語 |
英語
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論文のバージョン | author
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査読 |
有り
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DOI | |
Web of Science KeyUT |