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ID 50622
フルテキストURL
著者
Miyoshi, Shin-ichi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci
Jiyou, Wang Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci
Katoh, Keizo Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci
Senoh, Mitsutoshi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci
Mizuno, Tamaki Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci
Maehara, Yoko Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci
抄録
Vibrio vulnificus is a ubiquitous estuarine microorganism but causes fatal systemic infections in immunocompromised humans, cultured eels or shrimps. An extracellular metalloprotease VVP/VvpE has been reported to be a potential virulence factor of the bacterium; however, a few strains isolated from a diseased eel or shrimp were recently found to produce a serine protease termed VvsA, but not VVP/VvpE. In the present study, we found that these strains had lost the 80 kb genomic region including the gene encoding VVP/VvpE. We also purified VvsA from the culture supernatant through ammonium sulfate fractionation, gel filtration and ion-exchange column chromatography, and the enzyme was demonstrated to be a chymotrypsin-like protease, as well as those from some vibrios. The gene vvsA was shown to constitute an operon with a downstream gene vvsB, and several Vibrio species were found to have orthologues of vvsAB. These findings indicate that the genes vvp/vvpE and vvsAB might be mobile genetic elements.
キーワード
Polymerase chain reaction
Purification
Serine protease
Metalloprotease
Vibrio vulnificus
発行日
2012-04
出版物タイトル
World Journal of Microbiology & Biotechnology
28巻
4号
出版者
Springer
開始ページ
1633
終了ページ
1639
ISSN
0959-3993
資料タイプ
学術雑誌論文
プロジェクト
インド感染症共同研究
オフィシャル URL
http://dx.doi.org/10.1007/s11274-011-0969-y
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
author
査読
有り
DOI
Web of Sience KeyUT