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ID 50620
フルテキストURL
著者
Alam, Jawed Natl Inst Cholera & Enter Dis
Maiti, Sankar IISER
Ghosh, Prachetash Natl Inst Cholera & Enter Dis
De, Ronita Natl Inst Cholera & Enter Dis
Chowdhury, Abhijit Inst Post Grad Med Educ & Res, Sch Digest & Liver Dis
Das, Suryasnata St Johns Med Coll Hosp
Macaden, Ragini St Johns Med Coll Hosp
Devarbhavi, Harshad St Johns Med Coll Hosp
Ramamurthy, T. Natl Inst Cholera & Enter Dis
Mukhopadhyay, Asish K. Natl Inst Cholera & Enter Dis
抄録
A novel virulence factor, duodenal ulcer-promoting gene A (dupA), in Helicobacter pylori has been found to be associated with disease in certain populations but not in others. This study analysed a South-east Indian population as part of the debate about the relevance of dupA for the prediction of clinical outcomes. A total of 140 H. pylori strains isolated from duodenal ulcer (DU) (n=83) and non-ulcer dyspepsia (NUD) patients (n=57) were screened by PCR and dot-blot hybridization to determine the presence of the ORFs jhp0917 and jhp0918. Part of jhp0917-jhp0918 was sequenced to search for the C/T insertion that characterizes dupA and the levels of dupA transcripts were also assessed. The PCR and dot-blot results indicated the presence of jhp0917 and jhp0918 in 37.3% (31/83) and 12.2% (7/57) of H. pylori strains isolated from DU and NUD patients, respectively. Sequencing analysis showed insertion of a C at nt 1386 in the 3' region of jhp0917, forming the dupA gene in 35 strains. RT-PCR analysis detected the dupA transcript in 28 of these 35 strains. The expression level of the dupA transcript varied from strain to strain, as shown by real-time PCR. The results demonstrated that analysis based on PCR only for dupA may produce an erroneous interpretation. The prevalence of dupA was significantly greater among strains isolated from patients with DU than from patients with NUD in this population (P=0.001, odds ratio=4.26, confidence interval=1.60-11.74). Based on these findings, dupA can be considered a biomarker for DU patients in India. The reported discrepancies for this putative virulence marker in different populations may be due to the genome plasticity of H. pylori.
発行日
2012-09
出版物タイトル
Journal of Medical Microbiology
61巻
9号
開始ページ
1295
終了ページ
1302
ISSN
0022-2615
資料タイプ
学術雑誌論文
プロジェクト
インド感染症共同研究
オフィシャル URL
http://dx.doi.org/10.1099/jmm.0.038398-0
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
author
査読
有り
DOI
Web of Sience KeyUT