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ID 50619
フルテキストURL
著者
Sabui, Subrata Natl Inst Cholera & Enter Dis
Dutta, Sanjucta Natl Inst Cholera & Enter Dis
Debnath, Anusuya Natl Inst Cholera & Enter Dis
Ghosh, Avishek Natl Inst Cholera & Enter Dis
Hamabata, T. Natl Ctr Global Hlth & Med
Rajendran, K. Natl Inst Cholera & Enter Dis
Ramamurthy, T. Natl Inst Cholera & Enter Dis
Nataro, James P. Univ Virginia, Sch Med, Dept Pediat
Sur, Dipika Natl Inst Cholera & Enter Dis, Calcutta
Levine, Myron M. Univ Maryland, Sch Med, Ctr Vaccine Dev
Chatterjee, Nabendu Sekhar Natl Inst Cholera & Enter Dis
抄録
Enterotoxigenic Escherichia coli (ETEC) expressing the colonization factor CS6 is widespread in many developing countries, including India. The different allelic variants of CS6, caused by point mutations in its structural genes, cssA and cssB, are designated AIBI, AIIBII, AIIIBI, AIBII, and AIIIBII. A simple, reliable, and specific mismatch amplification mutation assay based on real-time quantitative PCR (MAMA-qPCR) was developed for the first time for the detection of CS6-expressing ETEC, along with the identification of allelic variations. The assay was based on mismatched nucleotide incorporation at the penultimate base at the 3' ends of the reverse primers specific for cssA and cssB and was validated using 38 CS6-expressing ETEC isolates. This strategy was effective in detecting all the alleles containing single-nucleotide polymorphisms. Using MAMA-qPCR, we also tested CS6 allelic variants in 145 ETEC isolates from children with acute diarrhea and asymptomatic infections, with the latter serving as controls. We observed that the AIBI and AIIIBI allelic variants were mostly associated with cases rather than controls, whereas the AIIBII variants were detected mostly in controls. In addition, the AIBI and AIIIBI alleles were frequently associated with ETEC harboring the heat-stable toxin gene (est) alone or with the heat-labile toxin gene (elt), whereas the AIIBII allele was predominant in ETEC isolates harboring the elt gene. This study may help in understanding the association of allelic variants in CS6-expressing ETEC with the clinical features of diarrhea, as well as in ETEC vaccine studies.
発行日
2012-04
出版物タイトル
Journal of Clinical Microbiology
50巻
4号
出版者
American Society for Microbiology
開始ページ
1308
終了ページ
1312
ISSN
0095-1137
NCID
AA00695531
資料タイプ
学術雑誌論文
プロジェクト
インド感染症共同研究
オフィシャル URL
http://dx.doi.org/10.1128/JCM.05424-11
言語
English
著作権者
Copyright © 2012, American Society for Microbiology. All Rights Reserved.
論文のバージョン
author
査読
有り
DOI
Web of Sience KeyUT