JaLCDOI 10.18926/AMO/52898
フルテキストURL 68_5_291.pdf
著者 Tsuzaki, Ryuichiro| Takaki, Akinobu| Yagi, Takahito| Ikeda, Fusao| Koike, Kazuko| Iwasaki, Yoshiaki| Shiraha, Hidenori| Miyake, Yasuhiro| Sadamori, Hiroshi| Shinoura, Susumu| Umeda, Yuzo| Yoshida, Ryuichi| Nobuoka, Daisuke| Utsumi, Masashi| Nakayama, Eiichi| Fujiwara, Toshiyoshi| Yamamoto, Kazuhide|
抄録 It is not known how the immune system targets hepatitis C virus (HCV)-infected HLA-mismatched hepatocytes under immune-suppressed conditions after orthotopic liver transplantation (OLT). In addition, the relationship between the HCV-specific immune response and IL28B variants as predictors of HCV clearance has not been well-characterized. We determined the IL28B polymorphisms for 57 post-OLT HCV carriers, and we assessed the HCV-specific immune responses by measuring the peripheral blood mononuclear cell-derived HCV-specific interferon-gamma (IFN-γ) response using an enzyme-linked immunospot assay. At 1-3 years after OLT, patients with no active hepatitis showed higher total spots on the immunospot assay. At>3 years after OLT, patients with resolved HCV showed higher levels of core, NS3, NS5A, and total spots compared to the chronic hepatitis patients. The IL28B major genotype in the donors correlated with higher spot counts for NS5A and NS5B proteins at 1-3 years after OLT. In the post-OLT setting, the HCV-specific immune response could be strongly induced in patients with no active hepatitis with an IL28B major donor or sustained virological response. Strong immune responses in the patients with no active hepatitis could only be maintained for 3 years and diminished later. It may be beneficial to administer IFN treatment starting 3 years after OLT, to induce the maximum immunological effect.
キーワード interferon gamma ELISPOT assay single nucleotide polymorphisms dendritic cell CD4 T cell
Amo Type Original Article
出版物タイトル Acta Medica Okayama
発行日 2014-10
68巻
5号
出版者 Okayama University Medical School
開始ページ 291
終了ページ 302
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 英語
著作権者 CopyrightⒸ 2014 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 25338486
Web of Science KeyUT 000343269300006
関連URL http://ousar.lib.okayama-u.ac.jp/metadata/53129
フルテキストURL WorldJHepatology_5_4_189-195.pdf
著者 Satoh, Daisuke| Yagi, Takahito| Nagasaka, Takeshi| Shinoura, Susumu| Umeda, Yuzo| Yoshida, Ryuichi| Utsumi, Masashi| Tanaka, Takehiro| Sadamori, Hiroshi| Fujiwara, Toshiyoshi|
キーワード Non-alcoholic fatty liver disease Pancreatoduodenectomy CD14 Endotoxin Kupffer cells
発行日 2013-04-27
出版物タイトル World Journal of Hepatology
5巻
4号
開始ページ 189
終了ページ 195
資料タイプ 学術雑誌論文
言語 英語
OAI-PMH Set 岡山大学
著作権者 ©2013 Baishideng Publishing Group Co., Limited. All rights reserved.
論文のバージョン publisher
DOI 10.4254/wjh.v5.i4.189
オフィシャル URL http://www.wjgnet.com/1948-5182/full/v5/i4/189.htm|
関連URL http://ousar.lib.okayama-u.ac.jp/metadata/51448