start-ver=1.4 cd-journal=joma no-vol=27 cd-vols= no-issue=24 article-no= start-page=8739 end-page=8747 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080706 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Drosophila blimp-1 is a transient transcriptional repressor that controls timing of the ecdysone-induced developmental pathway en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-fl is expressed in a temporally regulated manner following pulses of ecdysteroid and this precise expression is necessary for the development of Drosophila melanogaster. To understand how insect hormone ecdysteroids regulate the timing of FTZ-F1 expression, we purified a DNA binding regulator of ftz-fl. Mass spectroscopy analysis revealed this protein to be a fly homolog of mammalian B lymphocyte-induced maturation protein 1 (Blimp-1). Drosophila Blimp-1 (dBlimp-1) is induced directly by 20-hydroxyecdysone, and its product exists during high-ecdysteroid periods and turns over rapidly. Forced expression of dBlimp-1 and RNA interference analysis indicate that dBlimp-1 acts as a repressor and controls the timing of FTZ-F1 expression. Furthermore, its prolonged expression results in delay of pupation timing. These results suggest that the transient transcriptional repressor dBlimp-1 is important for determining developmental timing in the ecdysone-induced pathway.

en-copyright= kn-copyright= en-aut-name=AgawaYasuo en-aut-sei=Agawa en-aut-mei=Yasuo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SarhanMoustafa en-aut-sei=Sarhan en-aut-mei=Moustafa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KageyamaYuji en-aut-sei=Kageyama en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=AkagiKazutaka en-aut-sei=Akagi en-aut-mei=Kazutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TakaiMasayoshi en-aut-sei=Takai en-aut-mei=Masayoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HashiyamaKazuya en-aut-sei=Hashiyama en-aut-mei=Kazuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=WadaTadashi en-aut-sei=Wada en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=HandaHiroshi en-aut-sei=Handa en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=IwamatsuAkihiro en-aut-sei=Iwamatsu en-aut-mei=Akihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=HiroseSusumu en-aut-sei=Hirose en-aut-mei=Susumu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=UedaHitoshi en-aut-sei=Ueda en-aut-mei=Hitoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil= kn-affil=Department of Developmental Genetics, National Institute of Genetics and Department of Genetics, The Graduate University for Advanced Studies affil-num=2 en-affil= kn-affil=The Graduate School of Natural Science and Technology and Department of Biology, Faculty of Science, Okayama University affil-num=3 en-affil= kn-affil=Department of Developmental Genetics, National Institute of Genetics and Department of Genetics, The Graduate University for Advanced Studies affil-num=4 en-affil= kn-affil=The Graduate School of Natural Science and Technology and Department of Biology, Faculty of Science, Okayama University affil-num=5 en-affil= kn-affil=The Graduate School of Natural Science and Technology and Department of Biology, Faculty of Science, Okayama University affil-num=6 en-affil= kn-affil=Department of Developmental Genetics, National Institute of Genetics and Department of Genetics, The Graduate University for Advanced Studies affil-num=7 en-affil= kn-affil=Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology affil-num=8 en-affil= kn-affil=Integrated Research Institute, Tokyo Institute of Technology affil-num=9 en-affil= kn-affil=Protein Research Network, Inc. affil-num=10 en-affil= kn-affil=Department of Developmental Genetics, National Institute of Genetics and Department of Genetics, The Graduate University for Advanced Studies affil-num=11 en-affil= kn-affil=The Graduate School of Natural Science and Technology and Department of Biology, Faculty of Science, Okayama University en-keyword=SEQUENTIAL GENE ACTIVATION kn-keyword=SEQUENTIAL GENE ACTIVATION en-keyword=MATURATION PROTEIN-1 BLIMP-1 kn-keyword=MATURATION PROTEIN-1 BLIMP-1 en-keyword=STEROID-RECEPTOR SUPERFAMILY kn-keyword=STEROID-RECEPTOR SUPERFAMILY en-keyword=POLYTENE CHROMOSOMES kn-keyword=POLYTENE CHROMOSOMES en-keyword=SALIVARY-GLAND kn-keyword=SALIVARY-GLAND en-keyword=FUSHI-TARAZU kn-keyword=FUSHI-TARAZU en-keyword=EMBRYONIC-DEVELOPMENT kn-keyword=EMBRYONIC-DEVELOPMENT en-keyword=MOLECULAR-MECHANISM kn-keyword=MOLECULAR-MECHANISM en-keyword=TEMPORAL REGULATION kn-keyword=TEMPORAL REGULATION en-keyword=STAGE SPECIFICITY kn-keyword=STAGE SPECIFICITY END