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ID 58056
フルテキストURL
Thumnail fulltext.pdf 2.07 MB
著者
Jinno, Katsuya Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Hiramatsu, Bunta Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Tsunashima, Kenta Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Fujimoto, Kayo Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University
Sakai, Kenji Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Kaken ID
Kiwa, Toshihiko Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Kaken ID researchmap
Tsukada, Keiji Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University Kaken ID researchmap
抄録
Liquid-phase magnetic immunoassay (MIA) using magnetic nano-particles (MNPs) has been studied as a more rapid method compared to optical methods for inspecting proteins and viruses. MIA can estimate the number of conjugated antibodies without being washed differently from conventional optical immunoassay. However, in the case of the liquid phase, it is considered that the magnetic properties of MNPs are affected by physical properties such as viscosity and impurity substances such as biological substances contained in the blood. In this study, the effect of sodium chloride (NaCl) in buffer and serum solution was evaluated to reveal the effect of serum because the sodium (Na+) and chloride (Cl-) ions in the serum dominate ion balance of blood. The measurement results of AC magnetic susceptibility and a dynamic light scattering (DLS) showed that the aggregation of MNPs was largely affected by the concentration of NaCl. This effect of the NaCl could be explained by shielding of the surface charge of MNPs by ions in the solution. Although the concentrations of NaCl in the buffer and serum solution were almost same, we found that MNPs were aggregated more in their size for those in the serum solution because of other impurities, such as proteins. These results suggest evaluation of effects of the contaminants in serum and optimization of polymer coatings of MNPs could be important factors to realize measurements of magnetic immunoassay with high accuracy. (C) 2019 Author(s).
発行日
2019-12-20
出版物タイトル
AIP Advances
9巻
12号
出版者
American Institute of Physics
開始ページ
125317
ISSN
2158-3226
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
© Author(s) 2019
論文のバージョン
publisher
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1063/1.5130168
ライセンス
http://creativecommons.org/licenses/by/4.0/
助成機関名
日本学術振興会
助成番号
JP15H05764