ID | 53946 |
フルテキストURL | |
著者 |
Gulsan, Ara Sathi
Department of Biomaterials, Okayama University
Mahmoud, Farahat
Department of Biomaterials, Okayama University
Kazi, Anisur Rahman
Department of Biomaterials, Okayama University
Sakai, Takayoshi
Department of Oral-Facial Disorders, Osaka University
Hirano, Yoshiaki
Department of Chemical Engineering, Kansai University
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抄録 | In vitro fabricated biological tissue would be a valuable tool to screen newly synthesized drugs or understand the tissue development process. Several studies have attempted to fabricate biological tissue in vitro. However, controlling the growth and morphology of the fabricated tissue remains a challenge. Therefore, new techniques are required to modulate tissue growth. RGD (arginine-glycine-aspartic acid), which is an integrin-binding domain of fibronectin, has been found to enhance cell adhesion and survival; it has been used to modify substrates for in vitro cell culture studies or used as tissue engineering scaffolds. In addition, this study shows novel functions of the RGD peptide, which enhances tissue growth and modulates tissue morphology in vitro. When an isolated submandibular gland (SMG) was cultured on an RGD-modified alginate hydrogel sheet, SMG growth including bud expansion and cleft formation was dramatically enhanced. Furthermore, we prepared small RGD-modified alginate beads and placed them on the growing SMG tissue. These RGD-modified beads successfully induced cleft formation at the bead position, guiding the desired SMG morphology. Thus, this RGD-modified material might be a promising tool to modulate tissue growth and morphology in vitro for biological tissue fabrication.
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備考 | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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発行日 | 2015
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出版物タイトル |
Scientific reports
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巻 | 5巻
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出版者 | Nature Publishing Group
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開始ページ | 11468
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ISSN | 2045-2322
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資料タイプ |
学術雑誌論文
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オフィシャル URL | http://dx.doi.org/10.1038/srep11468
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関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/53945
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言語 |
英語
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著作権者 | © 2015 Nature Publishing Group
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論文のバージョン | publisher
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査読 |
有り
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DOI | |
PubMed ID | |
Web of Science KeyUT |