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ID 31714
JaLCDOI
フルテキストURL
著者
Kawada, Kazuhiko Okayama University
Yonei, Toshiro National Okayama Medical Center
Ueoka, Hiroshi Okayama University
Kiura, Katsuyuki Okayama University
Tabata, Masahiro Okayama University
Takigawa, Nagio Okayama University
Harada, Mine Kyushu University, Fukuoka
Tanimoto, Mitsune Okayama University
抄録

When the development of chemotherapeutic agents reaches the clinical trial stage, it is necessary to perform drug sensitivity tests quickly in order to select the most promising agents for the treatment of cancer. In order to assess the possibility of using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay as a substitute for the human tumor clonogenic assay (HTCA), we evaluated the correlation between the results obtained by these 2 assays in 5 human lung cancer cell lines. The correlation coefficient between the results of the HTCA and the MTT assay was 0.673, indicating a relatively good correlation. The correlation was most prominent in platinum analogues (r = 0.939) and good in anthracyclines/anthracenedione (r = 0.611). However, no significant correlation was observed in vinca alkaloids, etoposide, irinotecan, SN-38 (an active metabolite of irinotecan), and rhizoxin. The results of the MTT assay showed a high degree of correlation with those of the HTCA in predicting the sensitivity of cancer cell lines to platinum analogues, and anthracyclines/anthracenedione. These results suggest that the MTT assay may be more convenient and quickly performed than the HTCA and can replace HTCA in evaluating the effects of anticancer agents, especially the platinum analogues and anthracyclines/anthracenedione.

キーワード
chemosensitivity test
3-(4
5-dimethylthiazol-2-yl)-2
5-diphenyltertrazolium bromide (MTT) assay
clonogenic assay
Amo Type
Article
発行日
2002-06
出版物タイトル
Acta Medica Okayama
56巻
3号
出版者
Okayama University Medical School
開始ページ
129
終了ページ
134
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT