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ID 50975
フルテキストURL
著者
Miki, Yukari Okayama Univ, Grad Sch Hlth Sci, Dept Med Technol
Gion, Yuka Okayama Univ, Dept Pathol, Grad Sch Med Dent & Pharmaceut Sci
Mukae, Yuriko Kawasaki Coll Allied Hlth Profess, Dept Med Technol
Hayashi, Atsushi Okayama Univ, Grad Sch Hlth Sci, Dept Med Technol
Sato, Hiaki Okayama Univ, Grad Sch Hlth Sci, Dept Med Technol
Yoshino, Tadashi Okayama Univ, Dept Pathol, Grad Sch Med Dent & Pharmaceut Sci
Takahashi, Kiyoshi Okayama Univ, Grad Sch Hlth Sci, Dept Med Technol
抄録
Little is known about the S100B+ lymphocytes, which are unique human peripheral blood lymphocytes (PBL) containing the S100B protein. It has recently been shown that S100B is released from various types of S100B+ cells and exhibits varied cytokine-like activities. In this study, we precisely characterized the S100B+ lymphocytes of healthy adults with respect to the proportion in the whole PBL, immunophenotypes, function, and their S100B mRNA expression and also evaluated their S100B-releasing activity upon stimulation. S100B+ lymphocytes were detected in all individuals examined, and the proportion of S100B+ lymphocytes in the whole PBL ranged from 0.42% to 16.15% (mean, 4.21%). In addition, two subtypes of S100B+ lymphocytes, a CTL subtype (CD3+ CD8+ CD16-) and a NK subtype (CD3- CD8- CD16+), were detected. The majority of the CTL subtype of S100B+ lymphocytes expressed the alpha beta-T-cell receptor. Surprisingly, S100B mRNA was detected not only in S100B+ lymphocytes, but also in every S100B- lymphocytes, although the expression levels of S100B mRNA in S100B- lymphocytes were much lower than those of S100B+ lymphocytes. The CTL subtype of S100B+ lymphocytes exhibited blastic morphological changes, proliferated and released S100B upon stimulation with phytohemagglutinin. The NK subtype of S100B+ lymphocytes exhibited morphological NK activity when cocultivated with NK-sensitive target, K-562 cells. Thus, the CTL subtype of S100B+ lymphocytes exhibit the biological characteristics of T cells, while the NK subtype of S100B+ lymphocytes exhibit the characteristics of NK cells. These results suggest that S100B+ lymphocytes are a particular subtype of cytotoxic lymphocytes that play a unique role in antitumor immunity.
キーワード
S100B
peripheral blood
cytotoxic T cells
NK cells
発行日
2013-02
出版物タイトル
European Journal of Haematology
90巻
2号
出版者
Wiley-Blackwell
開始ページ
99
終了ページ
110
ISSN
0902-4441
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1111/ejh.12036
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/50874
言語
English
論文のバージョン
author
査読
有り
DOI
Web of Sience KeyUT