このエントリーをはてなブックマークに追加
ID 57558
フルテキストURL
著者
Ojima, Fumiya Department of Biology, The Graduate School of Natural Science and Technology, Okayama University
Saito, Yuka Department of Biology, The Graduate School of Natural Science and Technology, Okayama University
Tsuchiya, Yukiko Department of Biology, The Graduate School of Natural Science and Technology, Okayama University
Ogoshi, Maho Department of Biology, The Graduate School of Natural Science and Technology, Okayama University Kaken ID
Fukamachi, Hiroshi The Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
Inagaki, Kenichi The Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Kaken ID
Otsuka, Fumio The Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID
Takeuchi, Sakae Department of Biology, The Graduate School of Natural Science and Technology, Okayama University Kaken ID publons
Takahashi, Sumio Department of Biology, The Graduate School of Natural Science and Technology, Okayama University Kaken ID
抄録
We previously demonstrated that female Runx3 knockout (Runx3-/-) mice were anovulatory and their uteri were atrophic and that Runx3 mRNA was expressed in granulosa cells. To clarify how Runx3 regulates folliculogenesis and ovulation, we examine the effects of Runx3 knockout on the gene expression of growth factors associated with folliculogenesis and enzymes associated with steroidogenesis. In Runx3-/- mouse ovaries, the numbers of primary and antral follicles were lower than those in wild-type (wt) mice at 3 weeks of age, indicating that the loss of Runx3 affects folliculogenesis. The expression of genes encoding activin and inhibin subunits (Inha, Inhba and Inhbb) was also decreased in ovaries from the Runx3-/- mice compared with that in wt mice. Moreover, the expression of the genes Cyp11a1 and Cyp19a1 encoding steroidogenic enzymes was also decreased. In cultured granulosa cells from 3-week-old mouse ovaries, Cyp19a1 mRNA levels were lower in Runx3-/- mice than those in wt mice. Follicle-stimulating hormone (FSH) treatment increased Cyp19a1 mRNA levels in both wt and Runx3-/- granulosa cells in culture but the mRNA level in Runx3-/- granulosa cells was lower than that in wt ones, indicating that granulosa cells could not fully function in the absence of Runx3. At 3 weeks of age, gonadotropin α subunit, FSHβ subunit and luteinizing hormone (LH) β subunit mRNA levels were decreased in Runx3-/- mice. These findings suggest that Runx3 plays a key role in female reproduction by regulating folliculogenesis and steroidogenesis in granulosa cells.
キーワード
Estrogen
Follicle
Mouse
Ovary
Runx3
発行日
2018-10-30
出版物タイトル
Cell and Tissue Research
375巻
3号
出版者
Springer
開始ページ
743
終了ページ
754
ISSN
0302-766X
NCID
AA00600105
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
author
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1007/s00441-018-2947-2
Citation
Ojima, F., Saito, Y., Tsuchiya, Y. et al. Cell Tissue Res (2019) 375: 743. https://doi.org/10.1007/s00441-018-2947-2
助成機関名
日本学術振興会
助成番号
26440167