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ID 61290
フルテキストURL
fulltext.pdf 1.59 MB
著者
Tumewu, Stephany Angelia Graduate School of Environmental and Life Science, Okayama University
Matsui, Hidenori Graduate School of Environmental and Life Science, Okayama University
Yamamoto, Mikihiro Graduate School of Environmental and Life Science, Okayama University Kaken ID publons researchmap
Noutoshi, Yoshiteru Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Toyoda, Kazuhiro Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Ichinose, Yuki Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
抄録
γ-Aminobutyric acid (GABA) is a widely distributed non-proteinogenic amino acid that accumulates in plants under biotic and abiotic stress conditions. Recent studies suggested that GABA also functions as an intracellular signaling molecule in plants and in signals mediating interactions between plants and phytopathogenic bacteria. However, the molecular mechanisms underlying GABA responses to bacterial pathogens remain unknown. In the present study, a GABA receptor, named McpG, was conserved in the highly motile plant-pathogenic bacteria Pseudomonas syringae pv. tabaci 6605 (Pta6605). We generated a deletion mutant of McpG to further investigate its involvement in GABA chemotaxis using quantitative capillary and qualitative plate assays. The wild-type strain of Pta6605 was attracted to GABA, while the ΔmcpG mutant abolished chemotaxis to 10‍ ‍mM GABA. However, ΔmcpG retained chemotaxis to proteinogenic amino acids and succinic semialdehyde, a structural analog of GABA. Furthermore, ΔmcpG was unable to effectively induce disease on host tobacco plants in three plant inoculation assays: flood, dip, and infiltration inoculations. These results revealed that the GABA sensing of Pta6605 is important for the interaction of Pta6605 with its host tobacco plant.
キーワード
bacterial virulence
chemotaxis
GABA
plant-microbe interaction
Pseudomonas
発行日
2020
出版物タイトル
Microbes and Environments
35巻
4号
出版者
Japanese Society of Microbial Ecology
開始ページ
ME20114
ISSN
1342-6311
NCID
AA11173196
資料タイプ
学術雑誌論文
言語
英語
OAI-PMH Set
岡山大学
著作権者
© 2020 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles.
論文のバージョン
publisher
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1264/jsme2.ME20114
助成機関名
文部科学省
助成番号
26660035
19H02956