start-ver=1.4 cd-journal=joma no-vol=9 cd-vols= no-issue=8 article-no= start-page=1810 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200730 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Ligation of MHC Class II Induces PKC-Dependent Clathrin-Mediated Endocytosis of MHC Class II en-subtitle= kn-subtitle= en-abstract= kn-abstract=In addition to antigen presentation to CD4(+)T cells, aggregation of cell surface major histocompatibility complex class II (MHC-II) molecules induces signal transduction in antigen presenting cells that regulate cellular functions. We previously reported that crosslinking of MHC-II induced the endocytosis of MHC-II, which was associated with decreased surface expression levels in murine dendritic cells (DCs) and resulted in impaired activation of CD4(+)T cells. However, the downstream signal that induces MHC-II endocytosis remains to be elucidated. In this study, we found that the crosslinking of MHC-II induced intracellular Ca(2+)mobilization, which was necessary for crosslinking-induced MHC-II endocytosis. We also found that these events were suppressed by inhibitors of Syk and phospholipase C (PLC). Treatments with a phorbol ester promoted MHC-II endocytosis, whereas inhibitors of protein kinase C (PKC) suppressed crosslinking-induced endocytosis of MHC-II. These results suggest that PKC could be involved in this process. Furthermore, crosslinking-induced MHC-II endocytosis was suppressed by inhibitors of clathrin-dependent endocytosis. Our results indicate that the crosslinking of MHC-II could stimulate Ca(2+)mobilization and induce the clathrin-dependent endocytosis of MHC-II in murine DCs. en-copyright= kn-copyright= en-aut-name=MasakiKento en-aut-sei=Masaki en-aut-mei=Kento kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HirakiYuhji en-aut-sei=Hiraki en-aut-mei=Yuhji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OnishiHiroka en-aut-sei=Onishi en-aut-mei=Hiroka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SatohYuka en-aut-sei=Satoh en-aut-mei=Yuka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=RochePaul A. en-aut-sei=Roche en-aut-mei=Paul A. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TanakaSatoshi en-aut-sei=Tanaka en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=FurutaKazuyuki en-aut-sei=Furuta en-aut-mei=Kazuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Department of Immunobiology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Immunobiology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Immunobiology, Faculty of Pharmacy and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=4 en-affil=Department of Immunobiology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Experimental Immunology Branch, National Cancer Institute, National Institutes of Health kn-affil= affil-num=6 en-affil=Department of Pharmacology, Division of Pathological Sciences, Kyoto Pharmaceutical University kn-affil= affil-num=7 en-affil=Department of Immunobiology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences kn-affil= en-keyword=MHC-II kn-keyword=MHC-II en-keyword=dendritic cells kn-keyword=dendritic cells en-keyword=endocytosis kn-keyword=endocytosis en-keyword=crosslink kn-keyword=crosslink en-keyword=PKC kn-keyword=PKC END start-ver=1.4 cd-journal=joma no-vol=100 cd-vols= no-issue=11-12 article-no= start-page=1029 end-page=1034 dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=1988 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A case of primary thoracic hemangiopericytoma: correlation between Ga-67 scintigraphic and pathological findings kn-title=胸腔内原発のhemangiopericytomaの1例 ―(67)Gaシンチグラフィーと病理所見の対比― en-subtitle= kn-subtitle= en-abstract= kn-abstract=A 27-year-old male was admitted to our hospital with the chief complaint of dyspnea. Radiologically, he was found to have a large tumor shadow in the left hemithorax with pleural effusion. The first thoracotomy was done to resect the tumor on January 14, 1988. The second and third thoracotomies were done because of local recurrence in the left hemithorax. Histological diagnosis was of a hmangio-pericytoma. Before the third operation, Ga-67 scintigraphy was performed. Ga-67 accumulation was noted in parts of the tumor mass. Histological findings of the resected tumor were compared with the degree and location of Ga-67 accumulation. Histologically, Ga-67 accumulation occurred in areas of bleeding and necrosis in the tumor tissue, but not in viable parts of the tumor. Ga-67 accumulation usually occurs in viable tumor cells, but our findings were contrary to this generality. en-copyright= kn-copyright= en-aut-name=TanabeMasatada en-aut-sei=Tanabe en-aut-mei=Masatada kn-aut-name=田邉正忠 kn-aut-sei=田邉 kn-aut-mei=正忠 aut-affil-num=1 ORCID= en-aut-name=GohdaFuminori en-aut-sei=Gohda en-aut-mei=Fuminori kn-aut-name=合田文則 kn-aut-sei=合田 kn-aut-mei=文則 aut-affil-num=2 ORCID= en-aut-name=YodenMidori en-aut-sei=Yoden en-aut-mei=Midori kn-aut-name=余田みどり kn-aut-sei=余田 kn-aut-mei=みどり aut-affil-num=3 ORCID= en-aut-name=SakamotoKazuhiro en-aut-sei=Sakamoto en-aut-mei=Kazuhiro kn-aut-name=坂本和裕 kn-aut-sei=坂本 kn-aut-mei=和裕 aut-affil-num=4 ORCID= en-aut-name=MiyamotoTsutomu en-aut-sei=Miyamoto en-aut-mei=Tsutomu kn-aut-name=宮本勉 kn-aut-sei=宮本 kn-aut-mei=勉 aut-affil-num=5 ORCID= en-aut-name=SeoHiroyuki en-aut-sei=Seo en-aut-mei=Hiroyuki kn-aut-name=瀬尾裕之 kn-aut-sei=瀬尾 kn-aut-mei=裕之 aut-affil-num=6 ORCID= en-aut-name=KawasakiYukiko en-aut-sei=Kawasaki en-aut-mei=Yukiko kn-aut-name=川崎幸子 kn-aut-sei=川崎 kn-aut-mei=幸子 aut-affil-num=7 ORCID= en-aut-name=OhkawaSatoshi en-aut-sei=Ohkawa en-aut-mei=Satoshi kn-aut-name=大川元臣 kn-aut-sei=大川 kn-aut-mei=元臣 aut-affil-num=8 ORCID= en-aut-name=TamaiToyosato en-aut-sei=Tamai en-aut-mei=Toyosato kn-aut-name=玉井豊理 kn-aut-sei=玉井 kn-aut-mei=豊理 aut-affil-num=9 ORCID= en-aut-name=YamamotoShinya en-aut-sei=Yamamoto en-aut-mei=Shinya kn-aut-name=山本眞也 kn-aut-sei=山本 kn-aut-mei=眞也 aut-affil-num=10 ORCID= en-aut-name=TanakaSatoshi en-aut-sei=Tanaka en-aut-mei=Satoshi kn-aut-name=田中聰 kn-aut-sei=田中 kn-aut-mei=聰 aut-affil-num=11 ORCID= en-aut-name=KobayashiShouji en-aut-sei=Kobayashi en-aut-mei=Shouji kn-aut-name=小林省二 kn-aut-sei=小林 kn-aut-mei=省二 aut-affil-num=12 ORCID= affil-num=1 en-affil= kn-affil=香川医科大学放射線医学教室 affil-num=2 en-affil= kn-affil=香川医科大学放射線医学教室 affil-num=3 en-affil= kn-affil=香川医科大学放射線医学教室 affil-num=4 en-affil= kn-affil=香川医科大学放射線医学教室 affil-num=5 en-affil= kn-affil=香川医科大学放射線医学教室 affil-num=6 en-affil= kn-affil=香川医科大学放射線医学教室 affil-num=7 en-affil= kn-affil=香川医科大学放射線医学教室 affil-num=8 en-affil= kn-affil=香川医科大学放射線医学教室 affil-num=9 en-affil= kn-affil=香川医科大学放射線医学教室 affil-num=10 en-affil= kn-affil=香川医科大学第一外科教室 affil-num=11 en-affil= kn-affil=香川医科大学第一外科教室 affil-num=12 en-affil= kn-affil=香川医科大学第一病理学教室 en-keyword=胸腔内原発のhemangiopericytoma kn-keyword=胸腔内原発のhemangiopericytoma en-keyword=(67)Gaシンチグラフィー kn-keyword=(67)Gaシンチグラフィー END