ID | 53967 |
フルテキストURL | |
著者 |
Hamada, Yoshimasa
Graduate School of Natural Science and Technology, Okayama University
Bando, Tetsuya
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Nakamura, Taro
Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School
Ishimaru, Yoshiyasu
Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School
Mito, Taro
Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School
Noji, Sumihare
Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School
Tomioka, Kenji
Graduate School of Natural Science and Technology, Okayama University
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Kaken ID
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Ohuchi, Hideyo
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
ORCID
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抄録 | Hemimetabolous insects such as the cricket Gryllus bimaculatus regenerate lost tissue parts using blastemal cells, a population of dedifferentiated proliferating cells. The expression of several factors that control epigenetic modification is upregulated in the blastema compared with differentiated tissue, suggesting that epigenetic changes in gene expression might control the differentiation status of blastema cells during regeneration. To clarify the molecular basis of epigenetic regulation during regeneration, we focused on the function of the Gryllus Enhancer of zeste [Gb'E(z)] and Ubiquitously transcribed tetratricopeptide repeat gene on the X chromosome (Gb'Utx) homologues, which regulate methylation and demethylation of histone H3 lysine 27 (H3K27), respectively. Methylated histone H3K27 in the regenerating leg was diminished by Gb'E(z)RNAi and was increased by Gb'UtxRNAi. Regenerated Gb'E(z)RNAi cricket legs exhibited extra leg segment formation between the tibia and tarsus, and regenerated Gb'UtxRNAi cricket legs showed leg joint formation defects in the tarsus. In the Gb'E(z)RNAi regenerating leg, the Gb'dac expression domain expanded in the tarsus. By contrast, in the Gb'UtxRNAi regenerating leg, Gb'Egfr expression in the middle of the tarsus was diminished. These results suggest that regulation of the histone H3K27 methylation state is involved in the repatterning process during leg regeneration among cricket species via the epigenetic regulation of leg patterning gene expression.
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キーワード | Regeneration
Epigenetics
Histone H3K27
Gryllus bimaculatus
Polycomb
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発行日 | 2015-09
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出版物タイトル |
Development
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巻 | 142巻
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号 | 17号
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出版者 | Company of Biologists
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開始ページ | 2916
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終了ページ | 2927
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ISSN | 0950-1991
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NCID | AA10667805
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資料タイプ |
学術雑誌論文
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オフィシャル URL | http://dx.doi.org/10.1242/dev.122598
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関連URL | http://ousar.lib.okayama-u.ac.jp/metadata/53950
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言語 |
英語
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著作権者 | © 2015. The authors, Publication licensee The Company of Biologists Ltd
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論文のバージョン | publisher
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査読 |
有り
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DOI | |
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