start-ver=1.4
cd-journal=joma
no-vol=13
cd-vols=
no-issue=2
article-no=
start-page=108
end-page=117
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=20080921
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Primary cultures of chick osteocytes retain functional gap junctions between osteocytes and between osteocytes and osteoblasts
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=<p>The inaccessibility of osteocytes due to their embedment in the calcified bone matrix in vivo has precluded direct demonstration that osteocytes use gap junctions as a means of intercellular communication. In this article, we report successfully isolating primary cultures of osteocytes from chick calvaria, and, using anti-connexin 43 immunocytochemistry, demonstrate gap junction distribution to be comparable to that found in vivo. Next, we demonstrate the functionality of the gap junctions by (1) dye coupling studies that showed the spread of microinjected Lucifer Yellow from osteoblast to osteocyte and between adjacent osteocytes and (2) analysis of fluorescence replacement after photobleaching (FRAP), in which photobleaching of cells loaded with a membrane-permeable dye resulted in rapid recovery of fluorescence into the photobleached osteocyte, within 5 min postbleaching. This FRAP effect did not occur when cells were treated with a gap junction blocker (18 alpha-glycyrrhetinic acid), but replacement of fluorescence into the photobleached cell resumed when it was removed. These studies demonstrate that gap junctions are responsible for intercellular communication between adjacent osteocytes and between osteoblasts and osteocytes. This role is consistent with the ability of osteocytes to respond to and transmit signals over long distances while embedded in a calcified matrix. </p>

en-copyright=
kn-copyright=

en-aut-name=KamiokaHiroshi
en-aut-sei=Kamioka
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

en-aut-name=IshiharaYoshihito
en-aut-sei=Ishihara
en-aut-mei=Yoshihito
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=

en-aut-name=RisHans
en-aut-sei=Ris
en-aut-mei=Hans
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=

en-aut-name=MurshidSakhr A.
en-aut-sei=Murshid
en-aut-mei=Sakhr A.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=

en-aut-name=SugawaraYasuyo
en-aut-sei=Sugawara
en-aut-mei=Yasuyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=

en-aut-name=YamamotoTeruko Takano
en-aut-sei=Yamamoto
en-aut-mei=Teruko Takano
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=

en-aut-name=LimSoo-Siang
en-aut-sei=Lim
en-aut-mei=Soo-Siang
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=

affil-num=1
en-affil=
kn-affil=Department of Orthodontics and Dentofacial Orthopedics, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences

affil-num=2
en-affil=
kn-affil=Department of Orthodontics and Dentofacial Orthopedics, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences

affil-num=3
en-affil=
kn-affil=Department of Zoology and Integrated Microscopy Resource, University of Wisconsin

affil-num=4
en-affil=
kn-affil=Department of Orthodontics and Dentofacial Orthopedics, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences

affil-num=5
en-affil=
kn-affil=Department of Orthodontics and Dentofacial Orthopedics, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences

affil-num=6
en-affil=
kn-affil=Department of Orthodontics and Dentofacial Orthopedics, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences

affil-num=7
en-affil=
kn-affil=National Science Foundation
en-keyword=osteocytes
kn-keyword=osteocytes
en-keyword=osteoblasts
kn-keyword=osteoblasts
en-keyword=gap junctions
kn-keyword=gap junctions
en-keyword=intercellular communication
kn-keyword=intercellular communication
en-keyword=dye coupling
kn-keyword=dye coupling
en-keyword=FRAP
kn-keyword=FRAP
END

start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2004
dt-pub=20040325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=ニワトリ胚頭蓋骨の骨細胞の3次元的形態計測
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=

en-aut-name=SugawaraYasuyo
en-aut-sei=Sugawara
en-aut-mei=Yasuyo
kn-aut-name=菅原康代
kn-aut-sei=菅原
kn-aut-mei=康代
aut-affil-num=1
ORCID=

affil-num=1
en-affil=
kn-affil=岡山大学
END