| ID | 60542 |
| フルテキストURL | |
| 著者 |
Abe, Fumitaka
Division of Basic Research, Institute of Crop Science, NARO
Ishida, Yuji
Plant Innovation Center, Japan Tobacco Inc.
Hisano, Hiroshi
Institute of Plant Science and Resources, Okayama University
ORCID
Kaken ID
publons
researchmap
Endo, Masaki
Division of Applied Genetics, Institute of Agrobiological Sciences, NARO
Komari, Toshihiko
Plant Innovation Center, Japan Tobacco Inc.
Toki, Seiichi
Division of Applied Genetics, Institute of Agrobiological Sciences, NARO
Sato, Kazuhiro
Institute of Plant Science and Resources, Okayama University
ORCID
Kaken ID
publons
researchmap
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| 抄録 | Here, we describe a protocol for producing multiple recessive mutants via genome editing in hexaploid wheat (Triticum aestivum) cv. Fielder. Using Agrobacterium-delivered CRISPR/Cas9 and three sub-genome-specific primer sets, all possible combinations of single, double, and triple transgene-free mutants can be generated. The technique for acceleration of generation advancement with embryo culture reduces time for mutant production. The mutants produced by this protocol can be used for the analysis of gene function and crop improvement. For complete details on the use and execution of this protocol, please refer to Abe et al. (2019).
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| 発行日 | 2020-06-13
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| 出版物タイトル |
STAR Protocols
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| 巻 | 1巻
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| 号 | 2号
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| 出版者 | Cell Press
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| 開始ページ | 100053
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| ISSN | 2666-1667
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| 資料タイプ |
学術雑誌論文
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| 言語 |
英語
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| OAI-PMH Set |
岡山大学
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| 著作権者 | © 2020 The Author(s).
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| 論文のバージョン | publisher
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| DOI | |
| 関連URL | isVersionOf https://doi.org/10.1016/j.xpro.2020.100053
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| ライセンス | https://creativecommons.org/licenses/by/4.0/
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| オープンアクセス（出版社） |
OA
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| オープンアーカイブ（出版社） |
非OpenArchive
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