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ID 49093
フルテキストURL
著者
Matsumoto, Takashi Natl Inst Agrobiol Sci
Tanaka, Tsuyoshi Natl Inst Agrobiol Sci
Sakai, Hiroaki Natl Inst Agrobiol Sci
Amano, Naoki Natl Inst Agrobiol Sci
Kanamori, Hiroyuki Inst Soc Techno Innovat Agr Forestry & Fisheries
Kurita, Kanako Inst Soc Techno Innovat Agr Forestry & Fisheries
Kikuta, Ari Inst Soc Techno Innovat Agr Forestry & Fisheries
Kamiya, Kozue Inst Soc Techno Innovat Agr Forestry & Fisheries
Yamamoto, Mayu Inst Soc Techno Innovat Agr Forestry & Fisheries
Ikawa, Hiroshi Inst Soc Techno Innovat Agr Forestry & Fisheries
Fujii, Nobuyuki Hitachi Govt & Publ Corp Syst Engn Ltd
Hori, Kiyosumi Natl Inst Agrobiol Sci
Itoh, Takeshi Natl Inst Agrobiol Sci
抄録
Full-length cDNA (FLcDNA) libraries consisting of 172,000 clones were constructed from a two-row malting barley cultivar (Hordeum vulgare 'Haruna Nijo') under normal and stressed conditions. After sequencing the clones from both ends and clustering the sequences, a total of 24,783 complete sequences were produced. By removing duplicates between these and publicly available sequences, 22,651 representative sequences were obtained: 17,773 were novel barley FLcDNAs, and 1,699 were barley specific. Highly conserved genes were found in the barley FLcDNA sequences for 721 of 881 rice (Oryza sativa) trait genes with 50% or greater identity. These FLcDNA resources from our Haruna Nijo cDNA libraries and the full-length sequences of representative clones will improve our understanding of the biological functions of genes in barley, which is the cereal crop with the fourth highest production in the world, and will provide a powerful tool for annotating the barley genome sequences that will become available in the near future.
発行日
2011-05
出版物タイトル
Plant Physiology
156巻
1号
開始ページ
20
終了ページ
28
ISSN
0032-0889
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1104/pp.110.171579
言語
English
論文のバージョン
publisher
査読
有り
DOI
Web of Science KeyUT