JaLCDOI | 10.18926/47005 |
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フルテキストURL | mfe_36_1_117_121.pdf |
著者 | Shirosaki, Yuki| Tsuru, Kanji| Hayakawa, Satoshi| Osaka, Akiyoshi| Takashima, Seisuke| |
抄録 | γ-Methacryloxypropyltrimethoxysilane (γ-MPS) was grafted to silicone due to emulsion polymerization to induce Si-OH groups, in order to provide silicone with bioactivity spontaneous deposition of apatite in body fluid and to improve cytocompatibility. Apatite deposited on the grafted silicone within 7 days of soaking in 1.5 times as concentrated as the Kokubo solution. Osteoblastic cells (MC3T3-E1) were cultured on the specimens up to 7 days. After 5 days of culture, the number of MC3T3-E1 cells on the grafted specimen was much greater than that on the original specimen. These results indicated that the biocompatibility of silicone elastomer was improved by the grafting γ-MPS. |
出版物タイトル | Memoirs of the Faculty of Engineering, Okayama University |
発行日 | 2001-12 |
巻 | 36巻 |
号 | 1号 |
開始ページ | 117 |
終了ページ | 121 |
ISSN | 0475-0071 |
言語 | 英語 |
論文のバージョン | publisher |
NAID | 120003497031 |
JaLCDOI | 10.18926/15363 |
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フルテキストURL | Mem_Fac_Eng_OU_34_39.pdf |
著者 | 早川 聡| 城崎 由紀| 籔田 武司| 都留 寛治| Ohtsuki Chikara| 尾坂 明義| |
抄録 | Silicone elastomer was chemically treated at 60℃ for 7 days with 30 wt% H(2)O(2) solutions with or without TaCl(5) and soaked for various periods in a simulated body fluid(Kokubo solution) up to 21 days. Apatite formation ability of the surface of the silicone elastomer specimens was investigated with thin-film X-ray diffraction and FT-IR reflection spectroscopy. These silicone specimens did not deposit apatite or calcium phosphates, irrespective of chemical treatment. Osteoblast-like cells (MC3T3-El) derived from mouse were cultured on the specimens at 36.5℃ under 5%C0(2) and 95% humidity. Similar degree of proliferation of cells was observed at 7 days among three specimens, while the no treatment specimen after incubation for 5 days showed a lower degree of proliferation than the silicone treated with 30 wt% H(2)O(2) solutions with or without TaCl(5). Alkaline phosphatase activity of the cells proliferated on the no treatment specimen was lower than those of the silicone treated with 30 wt% H(2)O(2) solutions with or without TaCl(5). These results indicate that the cytotoxicity of the silicone could be improved by the chemical treatment with 30 wt% H(2)O(2) solutions with or without TaCl(5). |
出版物タイトル | Memoirs of the Faculty of Engineering, Okayama University |
発行日 | 2000-03-27 |
巻 | 34巻 |
号 | 1-2号 |
開始ページ | 39 |
終了ページ | 43 |
ISSN | 0475-0071 |
言語 | 英語 |
論文のバージョン | publisher |
NAID | 120002308019 |
著者 | 城崎 由紀| |
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発行日 | 2005-03-25 |
出版物タイトル | |
資料タイプ | 学位論文 |