start-ver=1.4 cd-journal=joma no-vol=7 cd-vols= no-issue=12 article-no= start-page=1842 end-page=1846 dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=201212 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Functional characterization of a novel plasma membrane intrinsic protein2 in barley en-subtitle= kn-subtitle= en-abstract= kn-abstract=Water homeostasis is crucial to the growth and survival of plants. Plasma membrane intrinsic proteins (PIPs) have been shown to be primary channels mediating water uptake in plant cells. We characterized a novel PIP2 gene, HvPIP2;8 in barley (Hordeum vulgare). HvPIP2;8 shared 72–76% identity with other HvPIP2s and 74% identity with rice OsPIP2;8. The gene was expressed in all organs including the shoots, roots and pistil at a similar level. When HvPIP2;8 was transiently expressed in onion epidermal cells, it was localized to the plasma membrane. HvPIP2;8 showed transport activity for water in Xenopus oocytes, however its interaction with HvPIP1;2 was not observed. These results suggest that HvPIP2;8 plays a role in water homeostasis although further functional analysis is required in future. en-copyright= kn-copyright= en-aut-name=ShibasakaMineo en-aut-sei=Shibasaka en-aut-mei=Mineo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SasanoSizuka en-aut-sei=Sasano en-aut-mei=Sizuka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=UtsugiSigeko en-aut-sei=Utsugi en-aut-mei=Sigeko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KatsuharaMaki en-aut-sei=Katsuhara en-aut-mei=Maki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Institute of Plant Science and Resources; Okayama University affil-num=2 en-affil= kn-affil=Institute of Plant Science and Resources; Okayama University affil-num=3 en-affil= kn-affil=Institute of Plant Science and Resources; Okayama University affil-num=4 en-affil= kn-affil=Institute of Plant Science and Resources; Okayama University en-keyword=aquaporin kn-keyword=aquaporin en-keyword=plasma membrane intrinsic protein kn-keyword=plasma membrane intrinsic protein en-keyword=PIP kn-keyword=PIP en-keyword=HvPIP2;8 kn-keyword=HvPIP2;8 en-keyword=water transport kn-keyword=water transport en-keyword=barley kn-keyword=barley en-keyword=Hordeum vulgare kn-keyword=Hordeum vulgare en-keyword=Xenopus laevis oocytes kn-keyword=Xenopus laevis oocytes END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue=2 article-no= start-page=137 end-page=143 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=1995 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Evaluation of Three Different Procedures for Extracting Adenylates from Barley Roots Prior to Luminometric Quantification kn-title=ルシフェリン―ルシフェラーゼ法によるアデニル酸定量のための大麦根からのサンプル抽出方法の検討 en-subtitle= kn-subtitle= en-abstract=高等植物根のアデニル酸をルシフェリン-ルシフェラーゼ法による定量に適する抽出法を捜すために、数種の抽出法を比べた。過塩素酸で抽出し、オクチルアミンで中和し、フロンの一種で洗う方法が最も抽出効果が高く、また比較的操作が簡便であった。 kn-abstract=Three methods were compared for extracting adenylates from barley roots prior to their quantification by a lumino-metric method. In respect of efficiency in extracting adenylates and easiness in handling, the best result was obtained in the root sample which was homogenized in perchloric acid, neutralized by mixing with octylamine dissolved in 1,1,2-trichloro-1,2,2-trifluoroethane and centrifuged. en-copyright= kn-copyright= en-aut-name=AkiyamaYoshiko en-aut-sei=Akiyama en-aut-mei=Yoshiko kn-aut-name=秋山佳子 kn-aut-sei=秋山 kn-aut-mei=佳子 aut-affil-num=1 ORCID= en-aut-name=ShibasakaMineo en-aut-sei=Shibasaka en-aut-mei=Mineo kn-aut-name=柴坂三根夫 kn-aut-sei=柴坂 kn-aut-mei=三根夫 aut-affil-num=2 ORCID= en-aut-name=KawasakiToshio en-aut-sei=Kawasaki en-aut-mei=Toshio kn-aut-name=河﨑利夫 kn-aut-sei=河﨑 kn-aut-mei=利夫 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=Adenylate extraction kn-keyword=Adenylate extraction en-keyword=ADP kn-keyword=ADP en-keyword=AMP kn-keyword=AMP en-keyword=ATP kn-keyword=ATP en-keyword=Barley roots kn-keyword=Barley roots END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue=2 article-no= start-page=129 end-page=135 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=1995 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effects of Mannosen on Hydroponically Grown Barley kn-title=水耕栽培におけるオオムギの生育に及ぼすマンノースの影響 en-subtitle= kn-subtitle= en-abstract=薄いマンノース溶液がオオムギの養分吸収に及ぼす影響を調べるために、水耕栽培実験を行った。マンノース添加の有無は植物体のカリウム及びリン酸含量にはほとんど影響しなかったが、植物体の形態に若干の影響を与えた。 kn-abstract=To examin the effects of mannose on iron absorption of barley roots,barley was hydroponically grown for 36 days in a greenhouse. Potassium and phosphate of barley plants grown in a diluted mannose solution were obserbed at similar as the controls. On the otherhand, some morphological changes were observed in mannose-treated barley plants. en-copyright= kn-copyright= en-aut-name=ShibasakaMineo en-aut-sei=Shibasaka en-aut-mei=Mineo kn-aut-name=柴坂三根夫 kn-aut-sei=柴坂 kn-aut-mei=三根夫 aut-affil-num=1 ORCID= en-aut-name=MiyataMasahiko en-aut-sei=Miyata en-aut-mei=Masahiko kn-aut-name=宮田將彦 kn-aut-sei=宮田 kn-aut-mei=將彦 aut-affil-num=2 ORCID= en-aut-name=AkiyamaYoshiko en-aut-sei=Akiyama en-aut-mei=Yoshiko kn-aut-name=秋山佳子 kn-aut-sei=秋山 kn-aut-mei=佳子 aut-affil-num=3 ORCID= en-aut-name=KawasakiToshio en-aut-sei=Kawasaki en-aut-mei=Toshio kn-aut-name=河﨑利夫 kn-aut-sei=河﨑 kn-aut-mei=利夫 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Barley kn-keyword=Barley en-keyword=Hydroponics kn-keyword=Hydroponics en-keyword=Mannose kn-keyword=Mannose en-keyword=Potassium kn-keyword=Potassium en-keyword=Phosphate kn-keyword=Phosphate END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue=2 article-no= start-page=149 end-page=157 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Increase in Cytochorome c and a 11.9 kDa protein in Submerged Rice Seedlings after Exposure to Air kn-title=イネ水中芽生えの酸素適応過程におけるチトクロムcと11.9kDaタンパク質の増加 en-subtitle= kn-subtitle= en-abstract=イネ水中芽生えを空気に触れさせた後のチトクロムc含量の変動を調べるために、米ぬかから精製したチトクロムcを用いて抗チトクロムc血清を作成した。ウェスタンプロット分析によると、水中芽生えにはチトクロムcは検出されなかったが、空気に触れてから6時間以降に検出された。酸素適応24時間の芽生えには生重量あたりで好気対照と同レベルのチトクロムcが存在した。抗血清作成の抗原として用いたチトクロムc標品は、A408/A280比の値(4.66)が高いことから、よく精製されていると考えられるが、抗血清はいくつかの他のポリペプチドと反応した。そのうちの一つはチトクロムcよりも抗血清に強く反応し、その分子量は11.9kDaであった。このポリペプチドは酸素適応過程で増加し、水中芽生えや好気対照では24時間酸素適応芽生えよりも少なかった。 kn-abstract=To examine the changes in cytocrome c content in submerged rice seedlings after exposure to air, antiserum was prepared against purified cytocrome c from rice bran. Western blottong analysis revealed that cytochrome c was detected 6 h after exposure to air, but not detected in submerged rice seedling. On a fresh weight basis, the same level of cytochrome c as that of the aerobic control was found in the 24-h-air adapted seedlings. judging from the high A408/A280 ratio (4.66),the cytochrome c preparation used as antigen was considered to be well purified. However, the antiserum reacted other several polypeptides. One of them reacted more strongly against the antisermu than cytochrome c and its molecular weight was estimated as 11.9 kDa. The polypeptide increased during air adaptation and the levels found in both submerged seedlings and aerobic control were lower than that in 24-h-air-adapted seedlings. en-copyright= kn-copyright= en-aut-name=ShibasakaMineo en-aut-sei=Shibasaka en-aut-mei=Mineo kn-aut-name=柴坂三根夫 kn-aut-sei=柴坂 kn-aut-mei=三根夫 aut-affil-num=1 ORCID= en-aut-name=UshimaruTakashi en-aut-sei=Ushimaru en-aut-mei=Takashi kn-aut-name=丑丸敬史 kn-aut-sei=丑丸 kn-aut-mei=敬史 aut-affil-num=2 ORCID= en-aut-name=OokuboKatsuyuki en-aut-sei=Ookubo en-aut-mei=Katsuyuki kn-aut-name=大久保勝之 kn-aut-sei=大久保 kn-aut-mei=勝之 aut-affil-num=3 ORCID= en-aut-name=TsuchidaShin-ichi en-aut-sei=Tsuchida en-aut-mei=Shin-ichi kn-aut-name=土田進一 kn-aut-sei=土田 kn-aut-mei=進一 aut-affil-num=4 ORCID= en-aut-name=TsujiHideo en-aut-sei=Tsuji en-aut-mei=Hideo kn-aut-name=辻英夫 kn-aut-sei=辻 kn-aut-mei=英夫 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 en-keyword=Air-adaptation kn-keyword=Air-adaptation en-keyword=Anti-cytochrome c serum kn-keyword=Anti-cytochrome c serum en-keyword=Cytochrome c kn-keyword=Cytochrome c en-keyword=Cytocrome c purification kn-keyword=Cytocrome c purification en-keyword=Oryza sativa kn-keyword=Oryza sativa END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue=2 article-no= start-page=135 end-page=147 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Changes in Mitochondrial Spectra of Submerged Rice Seedlings after Exposure to Air Gaussian Deconvolution Analysis kn-title=イネ水中芽生えの酸素適応過程におけるミトコンドリアスペクトルの変化Gaussian Deconvolutionによる解析 en-subtitle= kn-subtitle= en-abstract=吸収スペクトルをカーブアナリシスの手法を用いて解析し、水中で発芽した芽生えが空気に触れたときに生じるミトコンドリアチトクロム類の量的変化を調べた。アンチマンイシンA存在下でのコハク酸還元による差スペクトルには3種のチトクロムbが見出されるが、チトクロムc1とよく似た成分がかなりの寄与で存在していることが判った。その寄与を除くといずれの芽生えから単離したミトコンドリアにおいても、3種のチトクロムbは互いにほとんど同程度の吸収を示した。また、水中芽生え、酸素適応芽生え、好気芽生えから単離したミトコンドリアの間には、3種いずれのチトクロムbの吸収(タンパク質当たり)に関しても差がなかった。チトクロムcは水中芽生えでは好気芽生えの約半分の量が存在し、酸素適応過程で1.5倍に増加した。これはチトクロムaa3の変化と全く同じであった。チトクロム類の中には、酸素適応過程において酸素に対する応答の異なる2つのグループが存在することを示した。 kn-abstract=Quantitative changes in mitochondrial cytochromes of submerged rice seedlings after exposure to air were investigated using gaussian deconvolution analysis on absorption spectrum at liquid nitrogen temperature. Differnece spectra were obtained from subtracting the absorption spectra of oxidized mitochondria from reduced mitochondria by succinate with antimycin-A. they showed three kinds of b-type cytochrome and a spectral component similar to cytochrome c1. Subtraction of the cytochrome c1-like component from the spectrum by curve analysis showed that three cytochrome bs had similar absorption maximum and that on a mitochondrial protein basis they were constant during air-adaptation. Cytochrome c per mitochontorial protein in submerged seedlings was about half of that in aerobic seedlings and increased by 1.5-fold during air-adaptation. The change in cytochrome c content was in paralleled with that of cytochrome aa3. These findings revealed that there were two groups of cytochromes in the response to air-adaptation,i.e.,three cytochrome bs were constant and cytochoromes c and aa3 increased in parallel. en-copyright= kn-copyright= en-aut-name=ShibasakaMineo en-aut-sei=Shibasaka en-aut-mei=Mineo kn-aut-name=柴坂三根夫 kn-aut-sei=柴坂 kn-aut-mei=三根夫 aut-affil-num=1 ORCID= en-aut-name=TsujiHideo en-aut-sei=Tsuji en-aut-mei=Hideo kn-aut-name=辻英夫 kn-aut-sei=辻 kn-aut-mei=英夫 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Absorption spectrum kn-keyword=Absorption spectrum en-keyword=Air-adaptation kn-keyword=Air-adaptation en-keyword=Cytocrome kn-keyword=Cytocrome en-keyword=Gaussian deconvolution analysis kn-keyword=Gaussian deconvolution analysis en-keyword=Oryza sativa kn-keyword=Oryza sativa END