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ID 53011
フルテキストURL
著者
Mazaki, Tetsuro Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg
Shiozaki, Yasuyuki Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg
Yamane, Kentaro Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg
Yoshida, Aki Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg
Nakamura, Mariko Kibi Int Univ Jr Coll, Dept Hlth & Welf Program
Yoshida, Yasuhiro Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biomat
Zhou, Di RIKEN, Nano Med Engn Lab
Kitajima, Takashi RIKEN, Nano Med Engn Lab
Tanaka, Masato Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg ORCID Kaken ID publons researchmap
Ito, Yoshihiro RIKEN, Nano Med Engn Lab
Ozaki, Toshifumi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Orthopaed Surg Kaken ID publons researchmap
Matsukawa, Akihiro Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Pathol & Expt Med ORCID Kaken ID publons researchmap
抄録
Osteochondral injuries remain difficult to repair. We developed a novel photo-cross-linkable furfurylamine-conjugated gelatin (gelatin-FA). Gelatin-FA was rapidly cross-linked by visible light with Rose Bengal, a light sensitizer, and was kept gelled for 3 weeks submerged in saline at 37 degrees C. When bone marrow-derived stromal cells (BMSCs) were suspended in gelatin-FA with 0.05% Rose Bengal, approximately 87% of the cells were viable in the hydrogel at 24 h after photo-cross-linking, and the chondrogenic differentiation of BMSCs was maintained for up to 3 weeks. BMP4 fusion protein with a collagen binding domain (CBD) was retained in the hydrogels at higher levels than unmodified BMP4. Gelatin-FA was subsequently employed as a scaffold for BMSCs and CBD-BMP4 in a rabbit osteochondral defect model. In both cases, the defect was repaired with articular cartilage-like tissue and regenerated subchondral bone. This novel, photo-cross-linkable gelatin appears to be a promising scaffold for the treatment of osteochondral injury.
発行日
2014-03-25
出版物タイトル
Scientific Reports
4巻
ISSN
2045-2322
資料タイプ
学術雑誌論文
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/52967
言語
英語
著作権者
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論文のバージョン
publisher
査読
有り
DOI
Web of Science KeyUT