JaLCDOI 10.18926/AMO/30886
フルテキストURL fulltext.pdf
著者 Akagi, Tadaatsu| Takata, Hiroshi| Yoshino, Tadashi| Teramoto, Norihiro| Yano, Shoki| Oka, Takashi|
抄録 <p>Co-cultivation of thymus and spleen cells of Fisher and Lewis rats with lethally irradiated MT-2 cells harboring human T-cell leukemia virus type I (HTLV-I) resulted in the establishment of lymphoid cell lines, FIRT-1, FIRS-1, LERT-1, and LERS-1, respectively. Cells of these cell lines had rat T-cell characters as demonstrated by the positive reaction to monoclonal antibodies (MAbs) to rat T cell antigens (Thy 1 and pan T). They lacked surface immunoglobulins and strongly expressed rat interleukin-2 receptor antigen (Tac) and Ia antigen. Karyotypic analysis revealed that they had the normal rat karyotype in early cultures, but showed marked aneuploidy after long cultivation. None of them expressed HTLV gag proteins (p19 and p24) or virus particles, but they contained HTLV-I proviral DNA monoclonally and weakly expressed pX gene products (p40x). They were not transplantable into syngeneic newborn rats.</p>
キーワード human T-cell leukemia virus rat T cell immortalization
Amo Type Article
発行日 1989-06
出版物タイトル Acta Medica Okayama
43巻
3号
出版者 Okayama University Medical School
開始ページ 143
終了ページ 151
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2788356
Web of Sience KeyUT A1989AG01600002
JaLCDOI 10.18926/AMO/30764
フルテキストURL fulltext.pdf
著者 Ino, Hideo| Hayashi, Kazuhiko| Yanai, Hiroyuki| Teramoto, Norihiro| Koirala, Tirtha Raj| Chen, Hong-Li| Oka, Takashi| Yoshino, Tadashi| Takahashi, Kiyoshi| Akagi, Tadaastu|
抄録 <p>A simian cell line, Si-IIA, harboring Epstein-Barr-virus (EBV) -related herpesvirus (Si-IIA-EBV), produces malignant lymphoma in rabbits when administered by intravenous inoculation. In this study, we analyzed the Si-IIA-EBV genome and compared it with human EBV and herpesvirus macaca fascicularis 1 (HVMF 1 ), which is associated with B-cell lymphoma developing in SIV-infected immunosuppressed monkeys. DNA from Si-IIA-EBV was amplified by the polymerase chain reaction using three different primer pairs complementary to human EBV (B95-8) DNA; two of the primer pairs covered part of the long internal repeat 1 region (IR 1) and the third covered part of the BRRF 1 region. Direct sequencing of the three PCR products revealed that Si-IIA-EBV DNA had about 82% nucleotide homology to the human EBV DNA in all three regions and 92.4% homology to HVMF1 in the IR1 region. The blotting pattern by Southern blot analysis was different between Si-IIA-EBV and human EBV.</p>
キーワード Epstein-Barr virus HVMF 1 lymphoma ?monkey cell line PCR
Amo Type Article
発行日 1997-08
出版物タイトル Acta Medica Okayama
51巻
4号
出版者 Okayama University Medical School
開始ページ 207
終了ページ 212
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9284968
Web of Sience KeyUT A1997XU03200004
JaLCDOI 10.18926/AMO/32293
フルテキストURL fulltext.pdf
著者 Jin, Zaishun| Teramoto, Norihiro| Yoshino, Tadashi| Takada, Kenzo| Oka, Takashi| Hayashi, Kazuhiko| Akagi, Tadaatsu|
抄録 <p>It has been reported that Epstein-Barr virus (EBV) resides in resting B cells in vivo. However, an ideal in vitro system for studying EBV latent infection in vivo has not yet been established. In this study, a mantle cell lymphoma line, SP53, was successfully infected with a recombinant EBV containing a neomycin-resistant gene. The EBV-carrying SP53 cells were obtained by selection using G418. They expressed EBER-1, EBNAs, and LMP1; this expression pattern of the EBV genes was similar to that in a lymphoblastoid cell line (LCL). However, proliferation assay showed that the EBV-carrying SP53 cells have a doubling time of 73 h, compared with 57 h of SP53 cells. Transplantation of 10(8) SP53 cells to nude mice formed tumors in 4 of 10 mice inoculated, but the EBV-carrying SP53 cells did not. Unexpectedly, EBV infection reduced the proliferation and tumorigenicity of SP53 cells. However, the EBV-carrying SP53 cells showed higher resistance to apoptosis induced by serum starvation than did the SP53 cells. The inhibition of proliferation and the resistance to apoptosis induced in SP53 cells by EBV infection indicate that this cell line might to some extent provide a model of in vivo EBV reservoir cells.</p>
キーワード Epstein-Barr virus mantle cell lymphoma latent infection in vivo reservoir SP53 line
Amo Type Article
発行日 2000-10
出版物タイトル Acta Medica Okayama
54巻
5号
出版者 Okayama University Medical School
開始ページ 193
終了ページ 200
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 11061568
Web of Sience KeyUT 000090098600002
JaLCDOI 10.18926/AMO/32002
フルテキストURL fulltext.pdf
著者 Nakanishi, Tohru| Oka, Takashi| Akagi, Tadaatsu|
抄録 <p>The structure of the human genome is almost completely elucidated and the life sciences will now aim for a general and integrated study of gene expressions and the functional elucidation of proteins. In such a study, various new techniques have been developed, and DNA microarray technology is the most representative one. As for the DNA microarray techniques, several thousands to tens of thousands of gene segments are immobilized on a glass slide at high density, and cDNA probes prepared from specific cells or tissues are hybridized on the slides from which gene expression profiles are obtained at one sweep in a short time. The present development of this technique and its possible application to medicine-related fields are described.&#60;/P&#62;</p>
キーワード DNA microarray DNA chip human genome embryonic stem(ES)cell single nucleotide polymorphism(SNP)
Amo Type Review
発行日 2001-12
出版物タイトル Acta Medica Okayama
55巻
6号
出版者 Okayama University Medical School
開始ページ 319
終了ページ 328
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 11779093
Web of Sience KeyUT 000172838400001
JaLCDOI 10.18926/AMO/32097
フルテキストURL fulltext.pdf
著者 Koirala, Tirtha Raj| Hayashi, Kazuhiko| Jin, Zaishun| Onoda, Sachiyo| Tanaka, Takehiro| Oda, Wakako| Ichimura, Koichi| Ohara, Nobuya| Oka, Takashi| Yamada, Masao| Yoshino, Tadashi|
抄録 <p>Epstein-Barr virus (EBV)-related herpesvirus (Si-IIA-EBV) was serially transmitted for 3 passages from rabbit to rabbit of the opposite sex by blood transfusion, which subsequently induced virus-associated rabbit lymphomas. The virus could be transmitted by transfusion with 15-20 ml of whole blood (7/7) or irradiated blood (1/6) from the EBV-related virus-infected rabbits, but there was no transmission with transfusion of cell-free plasma (0/6) from the infected rabbits. Passive anti-EBV-VCA IgG (x 20 approximately x 10) titers decreased during the first 1-2 weeks in the transfused rabbits. The virus-transmitted rabbits showed a gradual increase in antibody titers ranging from peak titers of x 640 to x 2560 after 3 weeks of transfusion. The recipient origin of malignant lymphoma that developed in the first rabbit transfused by infected blood was confirmed by chromosomal analysis. This rabbit model thus shows that EBV-related herpesvirus is serially transmissible by blood transfusion and that transmission can not be completely prevented by irradiation of blood, but removal of blood cells is the best way to prevent transmission of EBV-related virus. Therefore, this animal model provides a convenient in vivo system for studies of the prevention and therapy of transfusion-related transmission of EBV and EBV-associated lymphoproliferative diseases in immunocompromised human beings.</p>
キーワード ?Epstein-Barr virus(EBV) rabbit lymphoproliferative diseases blood transfusion
Amo Type Article
発行日 2004-04
出版物タイトル Acta Medica Okayama
58巻
2号
出版者 Okayama University Medical School
開始ページ 67
終了ページ 74
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 15255507
Web of Sience KeyUT 000221043700002
著者 高田 尚良| 佐藤 康晴| 中村 直哉| 徳中 摩美| 三木 由香里| 菊池 イアーラ幸江| 五十嵐 和彦| 伊藤 悦郎| 張替 秀雄| 加藤 省一| 林 詠子| 岡 剛史| 星井 嘉信| 田利 晶| 岡田 裕之| Mohamado, ABD Alkader Lamia| 前田 嘉信| 谷本 光音| 木下 朝博| 吉野 正|
発行日 2013-08-01
出版物タイトル 岡山医学会雑誌
125巻
2号
資料タイプ 学術雑誌論文
著者 Abd Al Kader, Lamia| Oka, Takashi| Takata, Katsuyoshi| Sun, Xu| Sato, Hiaki| Murakami, Ichiro| Toji, Tomohiro| Manabe, Akihiro| Kimura, Hiroshi| Yoshino, Tadashi|
発行日 2013-11
出版物タイトル Virchows Archiv
463巻
5号
資料タイプ 学術雑誌論文