start-ver=1.4 cd-journal=joma no-vol=9 cd-vols= no-issue= article-no= start-page=102 end-page=114 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=2020 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Alpha-pinene and dizocilpine (MK-801) attenuate kindling development and astrocytosis in an experimental mouse model of epilepsy en-subtitle= kn-subtitle= en-abstract= kn-abstract=Understanding the molecular and cellular mechanisms involved during the onset of epilepsy is crucial for elucidating the overall mechanism of epileptogenesis and therapeutic strategies. Previous studies, using a pentylenetetrazole (PTZ)-induced kindling mouse model, showed that astrocyte activation and an increase in perineuronal nets (PNNs) and extracellular matrix (ECM) molecules occurred within the hippocampus. However, the mechanisms of initiation and suppression of these changes, remain unclear.
Herein, we analyzed the attenuation of astrocyte activation caused by dizocilpine (MK-801) administration, as well as the anticonvulsant effect of α-pinene on seizures and production of ECM molecules. Our results showed that MK-801 significantly reduced kindling acquisition, while α-pinene treatment prevented an increase in seizures incidences. Both MK-801 and α-pinene administration attenuated astrocyte activation by PTZ and significantly attenuated the increase in ECM molecules.
Our results indicate that astrocyte activation and an increase in ECM may contribute to epileptogenesis and suggest that MK-801 and α-pinene may prevent epileptic seizures by suppressing astrocyte activation and ECM molecule production. en-copyright= kn-copyright= en-aut-name=UenoHiroshi en-aut-sei=Ueno en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShimadaAtsumi en-aut-sei=Shimada en-aut-mei=Atsumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SuemitsuShunsuke en-aut-sei=Suemitsu en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MurakamiShinji en-aut-sei=Murakami en-aut-mei=Shinji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KitamuraNaoya en-aut-sei=Kitamura en-aut-mei=Naoya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=WaniKenta en-aut-sei=Wani en-aut-mei=Kenta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TakahashiYu en-aut-sei=Takahashi en-aut-mei=Yu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=MatsumotoYosuke en-aut-sei=Matsumoto en-aut-mei=Yosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=IshiharaTakeshi en-aut-sei=Ishihara en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of Medical Technology, Kawasaki University of Medical Welfare kn-affil= affil-num=2 en-affil=Division of Food and Nutrition, Nakamura Gakuen University Junior College kn-affil= affil-num=3 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=4 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=5 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=6 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=7 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=8 en-affil=Department of Neuropsychiatry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=9 en-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University kn-affil= affil-num=10 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= en-keyword=Epilepsy kn-keyword=Epilepsy en-keyword=Kindling kn-keyword=Kindling en-keyword=Pentylenetetrazol kn-keyword=Pentylenetetrazol en-keyword=Perineuronal nets kn-keyword=Perineuronal nets en-keyword=Wisteria floribunda kn-keyword=Wisteria floribunda END start-ver=1.4 cd-journal=joma no-vol=9 cd-vols= no-issue= article-no= start-page=5817 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201949 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Helping-Like Behaviour in Mice Towards Conspecifics Constrained Inside Tubes en-subtitle= kn-subtitle= en-abstract= kn-abstract=Prosocial behaviour, including helping behaviour, benefits others. Recently, helping-like behaviour has been observed in rats, but whether it is oriented towards rescue, social contact with others, or other goals remains unclear. Therefore, we investigated whether helping-like behaviour could be observed in mice similar to that in rats. Because mice are social animals widely used in neuroscience, the discovery of helping-like behaviour in mice would be valuable in clarifying the psychological and biological mechanisms underlying pro-sociability. We constrained mice inside tubes. Subject mice were allowed to move freely in cages with tubes containing constrained conspecifics. The subject mice released both cagemates and stranger mice but did not engage in opening empty tubes. Furthermore, the same behaviour was observed under aversive conditions and with anesthetised conspecifics. Interestingly, hungry mice opened the tubes containing food before engaging in tube-opening behaviour to free constrained conspecifics. Mice showed equal preferences for constrained and freely moving conspecifics. We demonstrated for the first time that mice show tube-opening behaviour. Furthermore, we partly clarified the purpose and motivation of this behaviour. An effective mouse model for helping-like behaviour would facilitate research on the mechanisms underlying prosocial behaviour. en-copyright= kn-copyright= en-aut-name=UenoHiroshi en-aut-sei=Ueno en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SuemitsuShunsuke en-aut-sei=Suemitsu en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MurakamiShinji en-aut-sei=Murakami en-aut-mei=Shinji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KitamuraNaoya en-aut-sei=Kitamura en-aut-mei=Naoya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=WaniKenta en-aut-sei=Wani en-aut-mei=Kenta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MatsumotoYosuke en-aut-sei=Matsumoto en-aut-mei=Yosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=IshiharaTakeshi en-aut-sei=Ishihara en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University kn-affil= affil-num=2 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=3 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=4 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=5 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=6 en-affil=Department of Neuropsychiatry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=7 en-affil=Graduate School of Health Sciences, Okayama University kn-affil= affil-num=8 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= END start-ver=1.4 cd-journal=joma no-vol=115 cd-vols= no-issue= article-no= start-page=108879 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201907 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Comprehensive behavioral study of the effects of vanillin inhalation in mice en-subtitle= kn-subtitle= en-abstract= kn-abstract=Vanillin is widely used in food and cosmetics, among other substances, for its sweet smell. However, the neuropsychological effects of vanillin inhalation have not been elucidated. In this study, we investigated the effect of vanillin inhalation on mouse behavior. First, we investigated whether the aroma of vanillin was attractive or repulsive for mice. Thereafter, the mice inhaled vanillin for 20?min before each test in a series of behavioral tests (elevated plus maze, open field, Y-maze, tail suspension, cotton bud biting, and Porsolt forced swim tests). In these tests, the mice showed a neutral response to vanillin. Mice that inhaled vanillin had a suppressed pain response in the hot plate test. In addition, the grip strength of the forelimbs of mice that inhaled vanillin was decreased. No significant differences were found between the mice inhaling vanillin and control mice in the open field, Y-maze, tail suspension, forced swimming, and aggression tests. These results show that vanillin inhalation has anti-nociceptive effects, similar to other routes of administration. The results also show that vanillin inhalation does not cause significant behavioral effects. en-copyright= kn-copyright= en-aut-name=UenoHiroshi en-aut-sei=Ueno en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShimadaAtsumi en-aut-sei=Shimada en-aut-mei=Atsumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SuemitsuShunsuke en-aut-sei=Suemitsu en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MurakamiShinji en-aut-sei=Murakami en-aut-mei=Shinji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KitamuraNaoya en-aut-sei=Kitamura en-aut-mei=Naoya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=WaniKenta en-aut-sei=Wani en-aut-mei=Kenta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TakahashiYu en-aut-sei=Takahashi en-aut-mei=Yu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=MatsumotoYosuke en-aut-sei=Matsumoto en-aut-mei=Yosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=FujiwaraYuko en-aut-sei=Fujiwara en-aut-mei=Yuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=IshiharaTakeshi en-aut-sei=Ishihara en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil=Department of Medical Technology, Kawasaki University of Medical Welfare kn-affil= affil-num=2 en-affil=Division of Food and Nutrition, Nakamura Gakuen University Junior College kn-affil= affil-num=3 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=4 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=5 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=6 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=7 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=8 en-affil=Department of Neuropsychiatry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=9 en-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University kn-affil= affil-num=10 en-affil=Department of Health and Sports Science, Kawasaki University of Medical Welfare kn-affil= affil-num=11 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= en-keyword=Anti-nociceptive kn-keyword=Anti-nociceptive en-keyword=Vanillin kn-keyword=Vanillin en-keyword=Essential oil kn-keyword=Essential oil en-keyword=Inhalation kn-keyword=Inhalation en-keyword=Analgesia kn-keyword=Analgesia END start-ver=1.4 cd-journal=joma no-vol=6 cd-vols= no-issue= article-no= start-page=1 end-page=17 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices en-subtitle= kn-subtitle= en-abstract= kn-abstract=In the developing central nervous system (CNS), extracellular matrix (ECM) molecules have regulating roles such as in brain development, neural-circuit maturation, and synaptic-function control. However, excluding the perineuronal net (PNN) area, the distribution, constituent elements, and expression level of granular ECM molecules (diffuse ECM) present in the mature CNS remain unclear. Diffuse ECM molecules in the CNS share the components of PNNs and are likely functional. As cortical functions are greatly region-dependent, we hypothesized that ECM molecules would differ in distribution, expression level, and components in a region- and layer-dependent manner. We examined the layer-specific expression of several chondroitin sulfate proteoglycans (aggrecan, neurocan, and brevican), tenascin-R, Wisteria floribunda agglutinin (WFA)-positive molecules, hyaluronic acid, and link protein in the somatosensory and piriform cortices of mature mice. Furthermore, we investigated expression changes in WFA-positive molecules due to aging. In the somatosensory cortex, PNN density was particularly high at layer 4 (L4), but not all diffuse ECM molecules were highly expressed at L4 compared to the other layers. There was almost no change in tenascin-R and hyaluronic acid in any somatosensory-cortex layer. Neurocan showed high expression in L1 of the somatosensory cortex. In the piriform cortex, many ECM molecules showed higher expression in L1 than in the other layers. However, hyaluronic acid showed high expression in deep layers. Here, we clarified that ECM molecules differ in constituent elements and expression in a region- and layer-dependent manner. Region-specific expression of ECM molecules is possibly related to functions such as region-specific plasticity and vulnerability. en-copyright= kn-copyright= en-aut-name=UenoHiroshi en-aut-sei=Ueno en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SuemitsuShunsuke en-aut-sei=Suemitsu en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MurakamiShinji en-aut-sei=Murakami en-aut-mei=Shinji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KitamuraNaoya en-aut-sei=Kitamura en-aut-mei=Naoya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=WaniKenta en-aut-sei=Wani en-aut-mei=Kenta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MatsumotoYosuke en-aut-sei=Matsumoto en-aut-mei=Yosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=IshiharaTakeshi en-aut-sei=Ishihara en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil=Department of Medical Technology, Kawasaki University of Medical Welfare kn-affil= affil-num=2 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=3 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=4 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=5 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=6 en-affil=Department of Neuropsychiatry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=7 en-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University kn-affil= affil-num=8 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= en-keyword=Extracellular matrix kn-keyword=Extracellular matrix en-keyword=Perineuronal nets kn-keyword=Perineuronal nets en-keyword=Piriform cortex kn-keyword=Piriform cortex en-keyword=Proteoglycans kn-keyword=Proteoglycans en-keyword=Somatosensory cortex kn-keyword=Somatosensory cortex en-keyword=Wisteria floribunda kn-keyword=Wisteria floribunda END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=6 article-no= start-page=e01945 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Anti-stress effects of the hydroalcoholic extract of Rosa gallica officinalis in mice en-subtitle= kn-subtitle= en-abstract= kn-abstract=Rosa gallica, a plant of the Rosa genus, has been used widely since the 13th century and is cultivated in many areas as a medicinal plant for the preparation of herbal medicines. However, details of the neuropsychological effects of R. gallica remain unclear; therefore we aimed to investigate the neuropsychological effects of a water-soluble extract of R. gallica in male C57BL/6N mice under normal conditions and under chronic stress. We administered a water-soluble extract of R. gallica to mice and performed a series of behavioral experiments to compare the treated animals with the untreated controls. No significant differences in activity level, anxiety-like behavior, depression-like behavior, body weight, and body temperature were observed between R. gallica-treated mice and control mice. However, in mice subjected to chronic stress, the observed decrease in activity was smaller in the R. gallica-treated mice than in the control mice. The oral administration of R. gallica did not affect the normal behavior of mice. However, when the mice were subjected to stress, R. gallica exerted an anti-stress effect. Therefore, R. gallica has potential as a medicinal plant for the purpose of stress prevention. en-copyright= kn-copyright= en-aut-name=UenoHiroshi en-aut-sei=Ueno en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShimadaAtsumi en-aut-sei=Shimada en-aut-mei=Atsumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SuemitsuShunsuke en-aut-sei=Suemitsu en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MurakamiShinji en-aut-sei=Murakami en-aut-mei=Shinji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KitamuraNaoya en-aut-sei=Kitamura en-aut-mei=Naoya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=WaniKenta en-aut-sei=Wani en-aut-mei=Kenta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MatsumotoYosuke en-aut-sei=Matsumoto en-aut-mei=Yosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=FujiwaraYuko en-aut-sei=Fujiwara en-aut-mei=Yuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=IshiharaTakeshi en-aut-sei=Ishihara en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of Medical Technology, Kawasaki University of Medical Welfare kn-affil= affil-num=2 en-affil=Division of Food and Nutrition, Nakamura Gakuen University Junior College kn-affil= affil-num=3 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=4 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=5 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=6 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= affil-num=7 en-affil=Department of Neuropsychiatry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=8 en-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University kn-affil= affil-num=9 en-affil=Department of Health and Sports Science, Kawasaki University of Medical Welfare kn-affil= affil-num=10 en-affil=Department of Psychiatry, Kawasaki Medical School kn-affil= en-keyword=Neuroscience kn-keyword=Neuroscience en-keyword=Physiology kn-keyword=Physiology END start-ver=1.4 cd-journal=joma no-vol=67 cd-vols= no-issue=3 article-no= start-page=135 end-page=143 dt-received= dt-revised= dt-accepted= dt-pub-year=2013 dt-pub=201306 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Somatosensory and Visual Deprivation Each Decrease the Density of Parvalbumin Neurons and Their Synapse Terminals in the Prefrontal Cortex and Hippocampus of Mice en-subtitle= kn-subtitle= en-abstract= kn-abstract=In the phenomenon known as cross-modal plasticity, the loss of one sensory system is followed by improved functioning of other intact sensory systems. MRI and functional MRI studies suggested a role of the prefrontal cortex and the temporal lobe in cross-modal plasticity. We used a mouse model to examine the effects of sensory deprivation achieved by whisker trimming and visual deprivation achieved by dark rearing in neonatal mice on the appearance of parvalbumin (PV) neurons and the formation of glutamic acid decarboxylase 67 (GAD67)-positive puncta around pyramidal neurons in the prefrontal cortex and hippocampus. Whisker trimming, but not dark rearing, decreased the density of PV neurons in the hippocampus at postnatal day 28 (P28). In the prefrontal cortex, whisker trimming and dark rearing decreased the density of PV neurons in layer 5/6 (L5/6) at P28 and in L2/3 at P56, respectively, whereas dark rearing increased the density of PV neurons in L5/6 at P56. Whisker trimming decreased the density of GAD67-positive puncta in CA1 of the hippocampus at both P28 and P56 and in L5/6 of the prefrontal cortex at P28. Dark rearing decreased the density of GAD67-positive puncta in CA1 of the hippocampus and in both L2/3 and L5/6 of the prefrontal cortex at P28, and in L2/3 of the prefrontal cortex at P56. These results demonstrate that somatosensory or visual deprivation causes changes in the PV-interneuronal network in the mouse prefrontal cortex and hippocampus. The results also suggest that the alteration of the PV-interneuronal network, especially in the prefrontal cortex, may contribute to cross-modal plasticity. en-copyright= kn-copyright= en-aut-name=UenoHiroshi en-aut-sei=Ueno en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ShoshiChikafumi en-aut-sei=Shoshi en-aut-mei=Chikafumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SuemitsuShunsuke en-aut-sei=Suemitsu en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=UsuiShinichi en-aut-sei=Usui en-aut-mei=Shinichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=SujiuraHiroko en-aut-sei=Sujiura en-aut-mei=Hiroko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University affil-num=2 en-affil= kn-affil=Department of Medical Technology, Kawasaki College of Allied Health Professions affil-num=3 en-affil= kn-affil=Department of Psychiatry, Kawasaki Medical University affil-num=4 en-affil= kn-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University affil-num=5 en-affil= kn-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University affil-num=6 en-affil= kn-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University en-keyword=sensory deprivation kn-keyword=sensory deprivation en-keyword=parvalbumin kn-keyword=parvalbumin en-keyword=glutamate decarboxylase (GAD67) kn-keyword=glutamate decarboxylase (GAD67) en-keyword=prefrontal cortex kn-keyword=prefrontal cortex en-keyword=hippocampus kn-keyword=hippocampus END start-ver=1.4 cd-journal=joma no-vol=66 cd-vols= no-issue=2 article-no= start-page=143 end-page=154 dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=201204 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Attenuated Sensory Deprivation-induced Changes of Parvalbumin Neuron Density in the Barrel Cortex of FcγRllB-deficient Mice en-subtitle= kn-subtitle= en-abstract= kn-abstract=Recent studies have demonstrated the important role of immune molecules in the development of neuronal circuitry and synaptic plasticity. We have detected the presence of FcγRllB protein in parvalbumin- containing inhibitory interneurons (PV neurons). In the present study, we examined the appearance of PV neurons in the barrel cortex and the effect of sensory deprivation in FcγRllB-deficient mice (FcγRllB-/-) and wild-type mice. There was no substantial difference in the appearance of PV neurons in the developing barrel cortex between FcγRllB-/- and wild-type mice. Sensory deprivation from immediately after birth (P0) or P7 to P12-P14 induced an increase in PV neurons. In contrast, sensory deprivation from P7 or P14 to P28, but not from P21 to P28, decreased PV neurons in wild-type mice. However, sensory deprivation from P0 or P7 to P12-P14 did not increase PV neurons and sensory deprivation from P7 or P14 to P28 did not decrease or only modestly decreased PV neurons in FcγRllB-/- mice. The results indicate that expression of PV is regulated by sensory experience and the second and third postnatal weeks are a sensitive period for sensory deprivation, and suggest that FcγRllB contributes to sensory experience-regulated expression of PV. en-copyright= kn-copyright= en-aut-name=WatanabeMakiko en-aut-sei=Watanabe en-aut-mei=Makiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=UenoHiroshi en-aut-sei=Ueno en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SuemitsuShunsuke en-aut-sei=Suemitsu en-aut-mei=Shunsuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YokobayashiEriko en-aut-sei=Yokobayashi en-aut-mei=Eriko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MatsumotoYosuke en-aut-sei=Matsumoto en-aut-mei=Yosuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=UsuiShinichi en-aut-sei=Usui en-aut-mei=Shinichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=SujiuraHiroko en-aut-sei=Sujiura en-aut-mei=Hiroko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University affil-num=2 en-affil= kn-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University affil-num=3 en-affil= kn-affil=Koyodai Hospital affil-num=4 en-affil= kn-affil=Department of Psychiatry, Kawasaki Medical University affil-num=5 en-affil= kn-affil=Department of Neuropsychiatry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=6 en-affil= kn-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University affil-num=7 en-affil= kn-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University affil-num=8 en-affil= kn-affil=Department of Medical Technology, Graduate School of Health Sciences, Okayama University en-keyword=parvalbumin kn-keyword=parvalbumin en-keyword=fast-spiking interneurons kn-keyword=fast-spiking interneurons en-keyword=FcγRllB kn-keyword=FcγRllB en-keyword=barrel cortex kn-keyword=barrel cortex en-keyword=sensory deprivation kn-keyword=sensory deprivation END start-ver=1.4 cd-journal=joma no-vol=7 cd-vols= no-issue=2 article-no= start-page=99 end-page=103 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=19970131 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=変形性膝関節炎患者膝関節液中の細胞外マトリックス成分と可溶性VCAM-1 kn-title=Extracellular matrix components and soluble VCAM-1 in synovial fluid with osteoarthritis of the knee en-subtitle= kn-subtitle= en-abstract=変形性膝関節炎(OA)患者は,高齢化社会の到来とともに今後ますます増加するものと考えられる｡OAの発症メカニズムについては,関節軟骨の破壊･修復やパンヌス形成に関わる多くの関連因子(サイトカイン,マトリックス分解酵素,分解酵素抑制因子,プロテオグリカン,活性酸素,細胞接着因子など)について,多面的に研究が進んでいる｡しかし,膝関節の炎症初期の病態のメカニズムについては,なお詳細に解明されておらず,関節炎初期の炎症指標の検索が今後さらに必要と考えられる｡本研究で私たちは, OA患者膝関節液中の細胞外マトリックス構成成分であるコラーゲン,へパラン硫酸プロテオグリカン,ウロン酸および炎症の最も初期に血管内皮細胞に出現するVCAM-1等の可溶化成分濃度を測定した｡患者膝関節液(SF)中の可溶性コラーゲン量と可溶性VCAM-1(sVCAM-1)量とは有意な正の相関関係が(ρ=0.652)観察された｡しかし,ヘパラン硫酸プロテオグリカン(HSPG)と可溶性コラーゲン(sCOLL)値間には有意な相関関係は確認されなかった｡また,一般に炎症の指標として用いられるCRP値とも比戟検討したが,CRP値と他の測定項目値とは一定の相関関係は存在しなかった。以上の結果より,OA患者のSF中の炎症マーカ-として,急性炎症時には従来の炎症指標蛋白質であるCRP値が有力であるというSipe JDの報告から推測するとsCOLLとsVCAM-1はCRP値と一定の相関を示さないことより,CRPの動態とは異なった炎症指標としての有用性が考えられる｡しかし,HSPGはエンドサイトシスにより速やかに代謝されるためSF中への解離が少なく,炎症指標としての可能性は低いと考えられる｡ kn-abstract=It is generally accepted that the number of patients with osteoarthritis (OA) of the knee must increase in the future because of the increase of the aged. The pathogenic mechanisms of OA haven't been elucidated yet, but it is supposed that mediate pannus may cause the factors for irreversible cartilage damage in the inflammatory process. Therefore it is very important to find and predict OA at the early stage. Now there were many indices to determine joint damage with OA, for example, cytokines including IL, TNF, TGF, matrix metalloproteinase (MMPs), tissue inhibitor of metalloproteinase (TIMPs), proteoglycan (PG), hyaluronate (HA), super oxide, adhesion molecules including, ICAM-1, VCAM-1 and PECAM. In this study, we measured that the soluble VCAM-1 (sVCAM-1), soluble collagen (sCOLL), heparansulfate proteoglycan (HSPG), uronic acis and CRP in synovial fluids (SF) with OA. A positive correlation between the level of sVCAM-1 and sCOLL (ρ = 0.652) was noted. However there was no factor showing a correlation with CRP. It was suggested that the levels of sVCAM-1 and sCOLL in the synovia fluid were active inflammation indices of knee damage, as the different aspects from CRP. The level of HSPG also showed a unique trend because HSPG on the cell surface was quickly digested endocytosis, so that it was not suitable for the inflammatory index in synovial fluid with OA. en-copyright= kn-copyright= en-aut-name=TohgeHiroko en-aut-sei=Tohge en-aut-mei=Hiroko kn-aut-name=唐下博子 kn-aut-sei=唐下 kn-aut-mei=博子 aut-affil-num=1 ORCID= en-aut-name=IchimuraMitsuko en-aut-sei=Ichimura en-aut-mei=Mitsuko kn-aut-name=一村光子 kn-aut-sei=一村 kn-aut-mei=光子 aut-affil-num=2 ORCID= en-aut-name=SakiyamaJunko en-aut-sei=Sakiyama en-aut-mei=Junko kn-aut-name=崎山順子 kn-aut-sei=崎山 kn-aut-mei=順子 aut-affil-num=3 ORCID= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name=岡本基 kn-aut-sei=岡本 kn-aut-mei=基 aut-affil-num=4 ORCID= en-aut-name=NakataYasunari en-aut-sei=Nakata en-aut-mei=Yasunari kn-aut-name=中田安成 kn-aut-sei=中田 kn-aut-mei=安成 aut-affil-num=5 ORCID= en-aut-name=EndoHiroshi en-aut-sei=Endo en-aut-mei=Hiroshi kn-aut-name=遠藤浩 kn-aut-sei=遠藤 kn-aut-mei=浩 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=2 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=3 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=4 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=5 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=6 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 en-keyword=変形性膝関節炎 (Osteoarthritis) kn-keyword=変形性膝関節炎 (Osteoarthritis) en-keyword=細胞外マトリックス (Extracellular matrix) kn-keyword=細胞外マトリックス (Extracellular matrix) en-keyword=可溶性VCAM-1 (Soluble VCAM-1) kn-keyword=可溶性VCAM-1 (Soluble VCAM-1) en-keyword=可溶性コラーゲン (Soluble Collagen) kn-keyword=可溶性コラーゲン (Soluble Collagen) en-keyword=可溶性ヘパラン硫酸プロテオグリカン (Soluble HSPG) kn-keyword=可溶性ヘパラン硫酸プロテオグリカン (Soluble HSPG) END start-ver=1.4 cd-journal=joma no-vol=8 cd-vols= no-issue=1 article-no= start-page=31 end-page=35 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=19970910 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Correlative study of hippocampal atrophy assessed by MRI and clinical features in temporal lobe epilepsy. kn-title=側頭葉てんかんの海馬萎縮と臨床像の検討 ―MRIを用いた海馬萎縮の簡易評価― en-subtitle= kn-subtitle= en-abstract=We studied hippocampal atrophy in 39 patients with temporal lobe epilepsy using MRI. The ratio (a) of the width of the hippocampus on the atrophic side to that on the contralateral side was measured in coronal sections of the short SE in MRI. According to this ratio, the patients were divided into three groups : 11 with hippocampal atrophy (a<0.8), 13 with borderline atrophy (0.8≦a≦0.9), and 15 with no hippocampal atrophy (a≧0.8). In the patients of the group with hippocampal atrophy, the clinical history of epilepsy tended to be long, and the site of hippocampal atrophy was consistent with that of interictal spike foci on the electroencephalogram in 9 out of 11 patients. However, there was no difference between the three groups, with regard to age at onset, age when MRI was conducted, frequency of seizures, generalized seizures, the types and doses of antiepileptic drugs used, history of neonatal asphyxia, intelligence and epilpetic psychosis. These results suggest that hippocampal atrophy in temporal lobe epilepsy may be related to repetition of epileptic dischargges in a localized part of the brain. kn-abstract=側頭葉てんかん患者39例の海馬萎縮をMRIを用いて評価し,臨床像との関連を検討した｡MRIの冠状断short SE像で海馬の幅を計測し,萎縮側海馬の村側海馬に対する比(a)を求め,海馬萎縮(+)群;a<0.8,11例,境界群;0.8≦a<0.9,13例,海馬萎縮(-)群;a≧0.9,15例の3群に分けた｡海馬萎縮(+)群で罹病期間が長い傾向があった｡また,発作間歇期脳波の焦点側は海馬委縮(+)群の11例中9例で萎縮側と一致した。しかし,発病年齢,MRI撮影時年齢,発作頻度,全般化発作の有無,抗てんかん薬総服用量,知能障害,精神症状,生下時仮死の有無については3群間で差が認められなかった｡この結果から,側頭葉てんかんにおける海馬萎縮は,生下時や全身けいれん発作時の低酸素状態によるものではなく,脳局所の反復するてんかん性発射と関連する可能性が示唆され,海馬萎縮の機序を考えるうえで興味深く思われた。 en-copyright= kn-copyright= en-aut-name=SatoKeiko en-aut-sei=Sato en-aut-mei=Keiko kn-aut-name=佐藤圭子 kn-aut-sei=佐藤 kn-aut-mei=圭子 aut-affil-num=1 ORCID= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name=岡本基 kn-aut-sei=岡本 kn-aut-mei=基 aut-affil-num=2 ORCID= en-aut-name=JojaIkuo en-aut-sei=Joja en-aut-mei=Ikuo kn-aut-name=上着郁夫 kn-aut-sei=上着 kn-aut-mei=郁夫 aut-affil-num=3 ORCID= en-aut-name=NakatsuTakeshi en-aut-sei=Nakatsu en-aut-mei=Takeshi kn-aut-name=中津武志 kn-aut-sei=中津 kn-aut-mei=武志 aut-affil-num=4 ORCID= en-aut-name=MorimotoKiyoshi en-aut-sei=Morimoto en-aut-mei=Kiyoshi kn-aut-name=森本清 kn-aut-sei=森本 kn-aut-mei=清 aut-affil-num=5 ORCID= en-aut-name=HayabaraToshiyuki en-aut-sei=Hayabara en-aut-mei=Toshiyuki kn-aut-name=早原敏之 kn-aut-sei=早原 kn-aut-mei=敏之 aut-affil-num=6 ORCID= en-aut-name=KurodaShigetoshi en-aut-sei=Kuroda en-aut-mei=Shigetoshi kn-aut-name=黒田重利 kn-aut-sei=黒田 kn-aut-mei=重利 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=国立療養所南岡山病院神経内科･臨床研究部 affil-num=2 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=3 en-affil= kn-affil=岡山大学医学部放射線医学教室 affil-num=4 en-affil= kn-affil=岡山市立市民病院神経内科 affil-num=5 en-affil= kn-affil=香川医科大学神経精神医学教室 affil-num=6 en-affil= kn-affil=国立療養所南岡山病院神経内科･臨床研究部 affil-num=7 en-affil= kn-affil=岡山大学医学部神経精神医学教室 en-keyword=側頭葉てんかん (temporal lobe epilepsy) kn-keyword=側頭葉てんかん (temporal lobe epilepsy) en-keyword=海馬委縮 (hippocampal atrophy) kn-keyword=海馬委縮 (hippocampal atrophy) en-keyword=罹病期間 (clinical history) kn-keyword=罹病期間 (clinical history) en-keyword=MRI kn-keyword=MRI en-keyword=発作間歇期脳波 (interictal EEG foci) kn-keyword=発作間歇期脳波 (interictal EEG foci) END start-ver=1.4 cd-journal=joma no-vol=8 cd-vols= no-issue=1 article-no= start-page=23 end-page=29 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=19970910 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A study on recording and analysis conditions of polysomnography for automated computer analysis of sleep stages. kn-title=睡眠ポリグラフ自動解析のための記録･解析条件の検討 en-subtitle= kn-subtitle= en-abstract=Recording and analysis conditions of polysomnography were examined in 10 young adult female by using sleep analysis computer Medilog SAC 847 system (Oxford). The quantity and percentage of slow wave sleep were consistent with those of visual inspection when the minimum delta amplitude was adjusted at ± 25〜31μV from the baseline. The gain amplitude of mandibular electromyogram dramatically affected recognition of REM stage by the computer. Percentage of REM sleep was almost same as that of visual inspection when the gain potentiometer was adjusted to read 50μV peak-to-peak calibration signal as 1.0V, while REM stage was not recognized when　the gain potentiometer was adjusted to 1.5V. In contrast, the gain amplitude of electropthalmogram　did not affect the recognition of REM stage. kn-abstract=自動解析装置を用いて終夜睡眠ポリグラフを解析する際の記録条件と解析条件を検討した。対象は若年成人女性10名で,解析には0xford社製睡眠ポリグラフ記録･解析装置Medilog SAC 847 Systemを用い,第3夜の記録を視察判定と比較した｡徐波睡眠の指標となるδ波の最小振幅基準を初期設定の基線から±31/μVと,これより低い±25/μVに設定すると,徐波睡眠量,徐波睡眠率とも視察による判定とほぼ同じ解析結果が得られた。眼球運動の入力感度はREM睡眠量,REM睡眠率に影響しなかったが,オトガイ筋電図の入力感度を初期設定の1.5VにするとREM睡眠がほとんどみれなくなる例が多く,1.0VではREM睡眠量がこれまでの報告より少ないものの,視察判定に近い結果が得られた｡以上の研究結果から,我々が用いた自動解析装置では,オトガイ筋電図の入力感度を1.0V,δ波の最小振幅基準を±25〜31/μVに設定すれば,視察判定とほぼ同じ解析結果が得られると考えられた｡ en-copyright= kn-copyright= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name=岡本基 kn-aut-sei=岡本 kn-aut-mei=基 aut-affil-num=1 ORCID= en-aut-name=InoueMakiko en-aut-sei=Inoue en-aut-mei=Makiko kn-aut-name=井上真紀子 kn-aut-sei=井上 kn-aut-mei=真紀子 aut-affil-num=2 ORCID= en-aut-name=NakashimaMakiko en-aut-sei=Nakashima en-aut-mei=Makiko kn-aut-name=中島麻紀子 kn-aut-sei=中島 kn-aut-mei=麻紀子 aut-affil-num=3 ORCID= en-aut-name=ItoSekiko en-aut-sei=Ito en-aut-mei=Sekiko kn-aut-name=伊藤昔子 kn-aut-sei=伊藤 kn-aut-mei=昔子 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=2 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=3 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=4 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 en-keyword=睡眠 (sleep) kn-keyword=睡眠 (sleep) en-keyword=終夜ポリグラフ (polysomnography) kn-keyword=終夜ポリグラフ (polysomnography) en-keyword=自動解析 (automated computer analysis) kn-keyword=自動解析 (automated computer analysis) en-keyword=記録条件 (recording conditions) kn-keyword=記録条件 (recording conditions) en-keyword=解析条件 (analysis condition) kn-keyword=解析条件 (analysis condition) END start-ver=1.4 cd-journal=joma no-vol=9 cd-vols= no-issue=2 article-no= start-page=105 end-page=111 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=19990226 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒスチジンタグを持つホスファカンコア蛋白の大腸菌での発現と精製 kn-title=Affinity purification of phosphacan core protein expressed in Escherichia coli as histidine-tagged fusion protein en-subtitle= kn-subtitle= en-abstract=コンドロイチン硫酸プロテオグリカンの一つであるホスファカンのコア蛋白の特定領域を,ヒスチジンタグ(His-tag)を持つ融合蛋白として大腸菌内で発現させニッケル-ニトリロ3酢酸(Ni-NTA)アフィニティ担体を用いて精製した｡ホスファカンコア蛋白のアミノ酸残基343-446(P3)及び1-340(P4)に相当するcDNA断片を,胎性18日目のラット脳由来のmRNAを鋳型としたPCRによって増幅した｡増幅された断片は発現ベクターpQE30に組み込まれ,これで大腸菌(M15[pREP4])を形質転換した｡His-tag融合蛋白の発現は形質転換株を1mM IPTG存在下で37℃,5時間培養することによって行われた｡His-tagged P3融合蛋白は可溶性蛋白質として発現し,Ni-NTA担体を用いて精製された.His-tagged P4融合蛋白は不溶性の封入体を形成したが,8M尿素によって可溶化され,変性条件下で同様に精製された｡ kn-abstract=Specific regions of core protein of phosphacan, one of the chondroitin sulfate proteoglycans, were expressed as fusion proteins with histidine-tag (His-tag) in Escherichia coli (E.coli) and were affinity purified using nickel-nitrilotriacetic acid (Ni-NTA) matrix. cDNA fragments encoding amino acid residues 343-446 (P3) and 1-340 (P4) of phosphacan core protein were amplified by polymerase chain reaction from E18 rat brain mRNA as template. The amplified products were subcloned into pQE30 vector and were introduced into E.coli strain M15 [pREP4] for the expression. The His-tagged fusion proteins were expressed by cultivating the transformants at 37℃ for 5h in the presence of 1mM IPTG. His-tagged P3 fusion protein (His-P3) was expressed as soluble form, and was purified using Ni-NTA matrix. His-tagged P4 fusion protein (His-P4) which was sequestered into insoluble inclusion bodies was treated with 8.0M urea to solubilize, and then was purified under denaturing conditions. en-copyright= kn-copyright= en-aut-name=ItoSekiko en-aut-sei=Ito en-aut-mei=Sekiko kn-aut-name=伊藤昔子 kn-aut-sei=伊藤 kn-aut-mei=昔子 aut-affil-num=1 ORCID= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name=岡本基 kn-aut-sei=岡本 kn-aut-mei=基 aut-affil-num=2 ORCID= en-aut-name=MoriShuji en-aut-sei=Mori en-aut-mei=Shuji kn-aut-name=森秀治 kn-aut-sei=森 kn-aut-mei=秀治 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部保健学科検査技術科学専攻 affil-num=2 en-affil= kn-affil=岡山大学医学部保健学科検査技術科学専攻 affil-num=3 en-affil= kn-affil=岡山大学医学部保健学科検査技術科学専攻 en-keyword=phosphacan (ホスファカン) kn-keyword=phosphacan (ホスファカン) en-keyword=core protein (コア蛋白) kn-keyword=core protein (コア蛋白) en-keyword=His-tagged proteins kn-keyword=His-tagged proteins en-keyword=recombinant protein (融合蛋白) kn-keyword=recombinant protein (融合蛋白) END start-ver=1.4 cd-journal=joma no-vol=9 cd-vols= no-issue=2 article-no= start-page=91 end-page=104 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=19990226 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A study on the correlation between the results of entrance examination and the academic record of the students in the department of laboratory technology kn-title=衛生技術学科入学者の高校評点,入学試験成績と入学後の学業成績の関係 ―入学者選抜,教育方法の改善に向けた自己点検･評価(1)― en-subtitle= kn-subtitle= en-abstract=The correlation between the school record in high school, result of entrance examination and the academic record, failure in promotion and withdrawal from school was examined in 361 students entered into department of laboratory technology from 1987 to 1995. 1) Thirty-nine students (10.8%) had failed in promotion, and 18 students (5%) had leaved from the school. The reason of withdrawal was refusal in 6 cases, and 4 students had changed their course to other universities. These cases were more frequent in the period until 1990 than in the period after 1991. 2) There were no differences in the school record in high school and the result of entrance examination among students who graduated scheduled period of attendance (regular students), those who failed in promotion, and those who had leaved the school. However, the students who failed in promotion had got higher score in entrance examination than regular students in 1987 and 1988. In contrast, regular students had got higher score in entrance examination than the students failed in promotion in 1991 and 1993. 3) The school record in high school could be cheked during the period from 1991 to1995. In this period, the students failed in promotion had got lower score in high school than regular students. Especially, the difference was significant in 1991 and 1993. 4) There was a significant positive correlation between the results in examination of general subjects and those in examination of technical and proffesional education. 5) The school records in high school had relatively weak positive correlation with the results of entrance examination. In contrast, both the school record in high school and result of entrance examination had clear positve correlations with both the results in examination of general subjects and technical and proffesional education. 6) However, the result of each subject of entrance examination, i.e. Japanese, Mathematics, English, and Science, had no or weak positive correlation with both the results in examination of general subjects and technical and proffesional education. 7) The students who had not passed the national examination of laboratory technologist had lower score in school record in high school, entrance examination, examination of general subjects and technical and proffesional education, but the diffenreces were not statistically significant. The results of the study indicate that there were students who failed in promotion or leaved school despite their excellent ability during four years following the establishment of School of Health Sciences, but thereafter the ability of students gradually became homogeneous and increased the students who failed in promotion because of insufficient ability. The highly positive correlation between the results in examination of general subjects and those in examination of technical and proffesional education indicate that the results of examination in the first year were directly reflected in the results of examination in second and third year, and that it is important to encourage the students's motivation and volition to study with ardor immediately after the entrance. The positive correlation of school record in high school and result of entrance examination with academic record after the entrance implicate the potential usefulness of school record in high school and result of entrance examination in determining the eligibility to enter the school. However, it is desirable to improve the subjects and problems of entrance examination because the result of each subject of entrance examination was poorly reflected in the academic record. kn-abstract=1987年4月から1995年4月の9年間に衛生技術学科へ入学した361名を対象に,高校評点,入学試験(学力試験)成績と,入学後の学業成績,退学,留年,臨床検査技師国家試験との関係を調べた｡ 1)入学者総数は361名で,このうち留年者が39名(10,8%),退学者が18名(5%)であった｡退学者の中には,実質入学辞退者が6名,4年制大学へ進路を変更した者が4名いたが,こうした退学者は1992年度までの入学者に多かった｡ 2)入学者全体では,予定卒業者,留年者,退学者の間で高校評点,入学試験成績には差がなかったが,入学年度ごとにみると,1987,1988年度は留年者が予定卒業者に比べて入学試験の総得点が高い傾向があり,逆に1991年,1993年度は予定卒業者に比べて有意に低かった｡ 3)高校評点は,データが入手できた1991年度以降一貫して留年者の評点が予定卒業者より低く, とくに1991年,1993年度は留年者の評点が有意に低かった｡ 4)留年者も含めて,一般教育科目の成績と専門科目の成績との間に有意を正の相関がみられた｡ 5)高校評点と入学試験成績との間には軽度の相関しかみられなかったが,高校評点および入学試験総合点は入学後の一般教育科目,専門科目の成績と高い正の相関を示した｡ 6)しかし,個々の入学試験科目の得点は,入学後の成績と相関しないか,軽度の相関しかみられなかった｡ 7)国家試験不合格者は,高校評点,入学試験総合点,一般科目平均点,専門科目平均点のいずれも合格者に比べて得点が低かったが,有意差はなかった｡以上の結果から,短期大学部開設当初は留年者,退学者の中に予定卒業者より学力のある学生がいたが,年を追うごとに入学者の学力が均質化し,学力が不十分なために留年する学生が多くなってきていると言える。一般教育科目の成績と専門科目の成績が強い正の相関を示すことは,1年次の成績がそのまま2,3年次の成績に反映されることを示しており,入学当初の動機付けと勉学意欲の喚起が重要なことを示している｡高校評点や学力試験の成績は入学後の成績とよく相関しており,学生選抜の有効な指標であると考えられたが,各入試科目との相関は乏しかったことから,個々の試験科目の問題内容については改善すべき課題があると思われた｡ en-copyright= kn-copyright= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name=岡本基 kn-aut-sei=岡本 kn-aut-mei=基 aut-affil-num=1 ORCID= en-aut-name=SakiyamaJunko en-aut-sei=Sakiyama en-aut-mei=Junko kn-aut-name=崎山順子 kn-aut-sei=崎山 kn-aut-mei=順子 aut-affil-num=2 ORCID= en-aut-name=AkatsukaKazuya en-aut-sei=Akatsuka en-aut-mei=Kazuya kn-aut-name=赤塚和也 kn-aut-sei=赤塚 kn-aut-mei=和也 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部保健学科検査技術科学専攻 affil-num=2 en-affil= kn-affil=岡山大学医学部保健学科検査技術科学専攻 affil-num=3 en-affil= kn-affil=福山臨床検査センター en-keyword=高校評点 (school record in high school) kn-keyword=高校評点 (school record in high school) en-keyword=入学試験成績 (result of entrance examination) kn-keyword=入学試験成績 (result of entrance examination) en-keyword=入学後の学業成績 (academic record) kn-keyword=入学後の学業成績 (academic record) en-keyword=留年 (failure in promotion) kn-keyword=留年 (failure in promotion) en-keyword=退学 (withdrawal from school) kn-keyword=退学 (withdrawal from school) END start-ver=1.4 cd-journal=joma no-vol=12 cd-vols= no-issue=1 article-no= start-page=63 end-page=70 dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=20011225 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Research on satisfaction of elderlies with dementia in health care facilities. kn-title=老人保健施設における痴呆老人の満足度調査 en-subtitle= kn-subtitle= en-abstract=The purpose of this study was to clarify if there is a relationship between the satisfaction of elderlies with dementia in health care facilities by subjective views of the investigator and their actions. The subjects of this investigation were 28 fully functional elderly clients with dementia who were living in 2 health care facilities. They or their family consented to take part in our research. 14 clients were chosen from each facilities. The investigators observed the clients for 48 hours and recorded where they were, with whom they were, what they did, as well as the client's level of satisfaction. The satisfaction levels were judged from the clients' appearances or attitudes by the investigations. There were five satisfaction including from 1 : unsatisfied to 5 : great satisfaction. The following three points were observed both in two facilities in common ; (1) the high satisfaction time period was "morning" when recreation was included. (2) the client received a high level of satisfaction when they were in the "recreation-room" or "outdoors", (3) the client received a high level of satisfaction whom they stayed with "their family" or "observers", when talking to someone or helping someone positively. Analysis of these results demonstrated there was similar pattern between the time of day and place and their companion or their actions among the subjects in two facilities. It can be assumed that it is able to observe elderlies with dementia satisfaction degree objectively. kn-abstract=2つの老人保健施設に入所している痴呆老人について,観察者による彼らの満足度とその時に観察された行動の関係を明らかにすることを目的とする｡調査対象者は,2つの老人保健施設に入所中で,本人または家族より調査への了系の得られた,歩行可能な痴呆老人各14名,合計28名である｡観察者は対象者の行動を48時間観察し,どこで誰と何を行っているかとその時の満足度を調査票に記録した｡満足度は対象者の表情や態度から観察者が判断し,大変満足をDとし,どちらとも言えないをB,大変不満足を@とした5段階評定で示した｡その結果以下の3点が明らかとなった｡ 1)両施設で満足度の高い時間帯は,レクリエーションが含まれる"午前中の時間"であった｡ 2)満足度が高いのは,居場所では,"レクリエーションルーム･屋外"であった｡ 3)同行者は,"家族","観察者",行動は,"話をする",手伝いをするなど"自発的な行為"が多くみられた｡以上により,2つの施設で満足度ごとの時間帯,居場所,同行者と行動が似通っていたことから,客観的に痴呆老人の満足度を把握することは可能であると考える｡ en-copyright= kn-copyright= en-aut-name=SumiyoshiKazuko en-aut-sei=Sumiyoshi en-aut-mei=Kazuko kn-aut-name=住吉和子 kn-aut-sei=住吉 kn-aut-mei=和子 aut-affil-num=1 ORCID= en-aut-name=KawataChieko en-aut-sei=Kawata en-aut-mei=Chieko kn-aut-name=川田智恵子 kn-aut-sei=川田 kn-aut-mei=智恵子 aut-affil-num=2 ORCID= en-aut-name=OkanoHatsue en-aut-sei=Okano en-aut-mei=Hatsue kn-aut-name=岡野初枝 kn-aut-sei=岡野 kn-aut-mei=初枝 aut-affil-num=3 ORCID= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name=岡本基 kn-aut-sei=岡本 kn-aut-mei=基 aut-affil-num=4 ORCID= en-aut-name=TakahashiKiyoshi en-aut-sei=Takahashi en-aut-mei=Kiyoshi kn-aut-name=高橋聖之 kn-aut-sei=高橋 kn-aut-mei=聖之 aut-affil-num=5 ORCID= en-aut-name=MoriShuji en-aut-sei=Mori en-aut-mei=Shuji kn-aut-name=森秀治 kn-aut-sei=森 kn-aut-mei=秀治 aut-affil-num=6 ORCID= en-aut-name=TsujimotoHisae en-aut-sei=Tsujimoto en-aut-mei=Hisae kn-aut-name=辻本久江 kn-aut-sei=辻本 kn-aut-mei=久江 aut-affil-num=7 ORCID= en-aut-name=SueEiko en-aut-sei=Sue en-aut-mei=Eiko kn-aut-name=須江英子 kn-aut-sei=須江 kn-aut-mei=英子 aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部保健学科 affil-num=2 en-affil= kn-affil=岡山大学医学部保健学科 affil-num=3 en-affil= kn-affil=岡山大学医学部保健学科 affil-num=4 en-affil= kn-affil=岡山大学医学部保健学科 affil-num=5 en-affil= kn-affil=岡山大学医学部保健学科 affil-num=6 en-affil= kn-affil=岡山大学医学部保健学科 affil-num=7 en-affil= kn-affil=老人保健施設白梅の丘 affil-num=8 en-affil= kn-affil=介護老人保健施設サンスクエア沼南 en-keyword=老人保健施設 (old people's health facilities) kn-keyword=老人保健施設 (old people's health facilities) en-keyword=痴呆老人 (elderlies with dementia) kn-keyword=痴呆老人 (elderlies with dementia) en-keyword=満足度 (satisfaction) kn-keyword=満足度 (satisfaction) END start-ver=1.4 cd-journal=joma no-vol=15 cd-vols= no-issue=1 article-no= start-page=9 end-page=14 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20041215 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The effect of Coicis semen and Rhizopus oligosporus-fermented Coicis semen (tempeh) on serum cholesterol in the rat kn-title=はとむぎ,はとむぎテンペのラットコレステロール代謝改善作用に関する研究 en-subtitle= kn-subtitle= en-abstract=The effect of Coicis semen and Rhizopus oligosporus-fermented Coicis semen (tempeh) on serum cholesterol fractions was examined in the rat. Twelve-week-old male Sprague-Dawley rats were received commercial MF meal (control group), Coicis semen-containing MF meal (Coicis semen group), or tempeh-containing MF meal (tempeh group). Cholesterol fractions were analyzed by high performance liquid chromatography at the start of experiment, and 2, 6, 18 weeks later. No difference was found in body weight nor total cholesterol among the three groups. LDL cholesterol was significantly lowered in the tempeh group at 2, 6, 18 weeks. LDL cholesterol/HDL cholesterol ratio also decreased at 2 and 18 weeks. In the both Coicis semen- and tempeh-fooded groups, LDL cholesterol and LDL cholesterol/HDL cholesterol ratio were lower than the control group although they were not statistically significant. No difference was found in HDL cholesterol among the three groups. The results suggest that tempeh may have a favorable effect on cholesterol metabolism. kn-abstract=はとむぎ(Coicis semen)をテンペ菌(Rhizopus oligosporus)で発酵したはとむぎテンペ(テンペ)の血清コレステロール代謝に対する作用を検討した｡生後12週齢のSprague-Dawley系雄ラットに市販固形飼料(コントロール群),はとむぎ混合飼料(はとむぎ群),テンペ混合飼料(テンペ群)を投与し,2,6,18週間後に高速液体クロマトグラフィー法で血清コレステロールを測定した｡体重増加,総コレステロールはいずれの時点でも3群間に差がなかったが,テンペ群では投与前と比較して2,6,18週間後にLDLコレステロールが有意に低下し,LDLコレステロール/HDLコレステロール比も2,18週間後に有意に低下した｡テンペ群はコントロール群と比較してもLDLコレステロールとLDLコレステロール/HDLコレステロール比が低く,はとむぎ群でも低い傾向がみられたが,有意差はなかった｡以上から,テンペがコレステロール代謝改善作用をもつことが示唆された｡ en-copyright= kn-copyright= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name=岡本基 kn-aut-sei=岡本 kn-aut-mei=基 aut-affil-num=1 ORCID= en-aut-name=UsuiShinichi en-aut-sei=Usui en-aut-mei=Shinichi kn-aut-name=臼井真一 kn-aut-sei=臼井 kn-aut-mei=真一 aut-affil-num=2 ORCID= en-aut-name=OkazakiMitsuyo en-aut-sei=Okazaki en-aut-mei=Mitsuyo kn-aut-name=岡崎三代 kn-aut-sei=岡崎 kn-aut-mei=三代 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部保健学科検査技術科学専攻 affil-num=2 en-affil= kn-affil=岡山大学医学部保健学科検査技術科学専攻 affil-num=3 en-affil= kn-affil=東京医科歯科大学教養部 en-keyword=はとむぎ (Coicis semen) kn-keyword=はとむぎ (Coicis semen) en-keyword=はとむぎテンペ (Rhizopus oligosporus, tempeh) kn-keyword=はとむぎテンペ (Rhizopus oligosporus, tempeh) en-keyword=コレステロール (cholesterol) kn-keyword=コレステロール (cholesterol) en-keyword=ラット (rat) kn-keyword=ラット (rat) END start-ver=1.4 cd-journal=joma no-vol=15 cd-vols= no-issue=2 article-no= start-page=63 end-page=69 dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050331 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Investigation of the tracheal suctioning-related microbialcolonization in home care patients with tracheotomy kn-title=気管内吸引を必要とする在宅療養患者の感染管理の実態 en-subtitle= kn-subtitle= en-abstract=Microbial colonization related to tracheal toilet was investigated in three home care patients who had tracheotomy with or without mechanical ventilation. We focused on the manipulation of suctioning tube to develop a effective and low-cost guidline for infection control. Tracheal suctioning was doned by wife in all the patients. Catheters used for suctioning was generally wiped with alcohol, washed in deionized or boiled water, and stored in alcohol-wiped container or immersed in 0.05% gluconate chlorhexizine. Catheter was changed into new one every 1.5 to 7 days. Samples were obtained from catheter, water used for washing, container as well as patients and their caregivers, and cultured on agar culture plate for Staphylococcus aureus, or Escherichia coli. Colonization of gauze around tracheotomy and water used for washing was detected in two patinets. E. coli and S. aureus were common organism colonizing gauze around tracheotomy. Therefore, it was suggested that the manipulation of the tracheal suctioning in home care influence on microbial colonization. kn-abstract=気管内吸引を必要とする在宅療養患者に対し,安全で経済的な感染管理方法を検討するため,在宅ケアにおける気管内吸引の実態と,吸引関連物品の細菌学的調査を実施した。対象は気管切開がされ,医療依存度の高い患者3名であり,気管内吸引は全て患者の妻が行っていた。吸引後のカテーテルは外側をアルコール綿で清拭し,内腔を洗浄水で洗浄していた。カテーテルは,0.05%グルコン酸クロルヘキシジンに浸漬あるいは,清潔な乾燥容器に保管されていた。カテーテルの交換時期は,1.5日から7日とばらつきがあった。カテーテル,洗浄水,浸潰液等の試料を,一般細菌,黄色ブドウ球菌,大腸菌群の寒天培地で培養したところ,各試料から一般細菌,黄色ブドウ球菌,大腸菌群が検出されたが,カテーテルの保管方法の違いによる細菌汚染に差は認められなかった｡また,気管切開部ガーゼからは,一般細菌,黄色ブドウ球菌,大腸菌群が検出された｡これらより,気管内吸引カテーテルおよび,洗浄水や浸溝用消毒液の清潔管理の必要性が示唆された｡ en-copyright= kn-copyright= en-aut-name=WatanabeKumi en-aut-sei=Watanabe en-aut-mei=Kumi kn-aut-name=渡邉久美 kn-aut-sei=渡邉 kn-aut-mei=久美 aut-affil-num=1 ORCID= en-aut-name=KanzakiHitomi en-aut-sei=Kanzaki en-aut-mei=Hitomi kn-aut-name=菅崎仁美 kn-aut-sei=菅崎 kn-aut-mei=仁美 aut-affil-num=2 ORCID= en-aut-name=SendaYoshiko en-aut-sei=Senda en-aut-mei=Yoshiko kn-aut-name=千田好子 kn-aut-sei=千田 kn-aut-mei=好子 aut-affil-num=3 ORCID= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name=岡本基 kn-aut-sei=岡本 kn-aut-mei=基 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部保健学科看護学専攻 affil-num=2 en-affil= kn-affil=岡山訪問看護ステーション看護協会 affil-num=3 en-affil= kn-affil=岡山大学医学部保健学科看護学専攻 affil-num=4 en-affil= kn-affil=岡山大学医学部保健学科検査技術科学専攻 en-keyword=感染管理 (Infection control) kn-keyword=感染管理 (Infection control) en-keyword=気管内吸引 (tracheal suctioning) kn-keyword=気管内吸引 (tracheal suctioning) en-keyword=在宅ケア (home care) kn-keyword=在宅ケア (home care) en-keyword=介護者 (caregiver) kn-keyword=介護者 (caregiver) END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue= article-no= start-page=79 end-page=85 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=19950131 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Comparative studies of proliferating cell nuclear antigen and bromodeoxyuridine staining pattern in rat regenerating liver cells kn-title=ラット再生肝組織におけるProliferating Cell Nuclear AntigenとBromodeoxyuridineの染色性についての比較研究 ―固定条件の影響について― en-subtitle= kn-subtitle= en-abstract=Immunohistochemical staining pattern of proliferating cell nuclear antigen (PCNA) was examined in rat regenarating liver specimens fixed under the various conditions (10% buffered formalin for 6 hr, 1% paraformaldehyde for 6 hr, 100% methanol for 4 hr, 70% ethanol for 6 hr, 100% acetone for 2 hr and Carnoy solution for 4 hr). Most distinct granular staining pattern of PCNA was observed in fixation with 100% methanol or 70% ethanol. Specimens fixed with 10% buffered formalin or 1% paraformaldehyde showed both distinct granular and slight diffuse pattern of PCNA staining. Specimens fixed with 100% acetone showed only slight diffuse staining. Specimens fixed with Carnoy solution showed little staining. Number of PCNA positive cells in the specimens fixed with 100% methanol or 70% ethanol on 24 hours after hepatectomy coincided closely with those of BrdU positive cells, which is known to be a representative marker of cell proliferation. In contrast, dissociative result was obtained in specimens fixed with 10% buffered formalin, 1% paraformaldehyde, 100% acetone or Carnoy solution when numbers of PCNA positive cells were compared with those of BrdU. These findings suggest that tissue specimens fixed with 100% methanol or 70% ethanol is useful for evaluating the extent of cell proliferation. kn-abstract=種々の条件下（10％緩衝ホルマリン・6時間、1％パラホルムアルデヒド・6時間、100％メタノール・4時間、70％エタノール・6時間、100％アセトン・2時間、カルノア液・4時間）で固定したラット再生肝組織に免疫組織染色を施し、Proliferating cell nuclear antigen （PCNA）の核内染色パターンについての検討を行った。その結果、100％メタノール及び70％エタノールで固定した場合には、明瞭な顆粒状の染色像が得られた。10％緩衝ホルマリン及び1％パラホルムアルデヒドの場合には、明瞭な顆粒状並びに弱いびまん性の染色像が得られた。S期に対する代表的なマーカーとして知られるBromodeoxyuridine （BrdU）の染色像との比較のもと、再生肝組織中のPCNA陽性細胞の数とBrdU陽性細胞の数を再生ピーク時の術後1日目で検討したところ、100％メタノール及び70％エタノールで固定した際の陽性細胞数が、最もBrdUの所見と一致した。しかし、10％緩衝ホルマリン、1％パラホルムアルデヒド、100％アセトン、カルノア液で固定した場合には、BrdUの所見と著しい解離が見られた。これらの知見は、PCNAを指標に細胞増殖を評価する上で、100％メタノール、70％エタノールでの固定が有用であることを示唆する。 en-copyright= kn-copyright= en-aut-name=SakiyamaJunko en-aut-sei=Sakiyama en-aut-mei=Junko kn-aut-name=ｱ山順子 kn-aut-sei=ｱ山 kn-aut-mei=順子 aut-affil-num=1 ORCID= en-aut-name=MoriShuji en-aut-sei=Mori en-aut-mei=Shuji kn-aut-name=森秀治 kn-aut-sei=森 kn-aut-mei=秀治 aut-affil-num=2 ORCID= en-aut-name=IchimuraMituko en-aut-sei=Ichimura en-aut-mei=Mituko kn-aut-name=一村光子 kn-aut-sei=一村 kn-aut-mei=光子 aut-affil-num=3 ORCID= en-aut-name=TohgeHiroko en-aut-sei=Tohge en-aut-mei=Hiroko kn-aut-name=唐下博子 kn-aut-sei=唐下 kn-aut-mei=博子 aut-affil-num=4 ORCID= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name=岡本基 kn-aut-sei=岡本 kn-aut-mei=基 aut-affil-num=5 ORCID= en-aut-name=EndoHiroshi en-aut-sei=Endo en-aut-mei=Hiroshi kn-aut-name=遠藤浩 kn-aut-sei=遠藤 kn-aut-mei=浩 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=2 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=3 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=4 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=5 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=6 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 en-keyword=PCNA kn-keyword=PCNA en-keyword=BrdU kn-keyword=BrdU en-keyword=免疫組織染色 (immunohistochemical staining) kn-keyword=免疫組織染色 (immunohistochemical staining) en-keyword=再生肝 (regenerating liver) kn-keyword=再生肝 (regenerating liver) END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue= article-no= start-page=37 end-page=45 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=19940131 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Utilization of a serum-free primary culture of cortical neurons by using cyclodextrins in neurobiological research en-subtitle= kn-subtitle= en-abstract= kn-abstract=神経生物学的研究における基本的分析系の確立を目的として、シクロデキストリン（CD）を用いたラット大脳皮質神経細胞の初代培養を試みた。β-およびγ-CDは、無血清培地（ダルベッコ改変MEM/ハム培地）中で胎生16および18日目ラットの神経細胞を11日以上10％胎児ウシ血清を加えた培地中と同じ程度に生存させたが、α-CDには生存維持効果が無かった。β-CDはγ-CDより安定した生存維持効果を示したが、胎生21日目ラットの神経細胞を用いた場合は有意に生存率が低下し、新生児ラットでは生存維持効果が無かった。β-CDを用いた無血清培養では10％血清培地中と比べて神経突起の伸展が悪かったが、ときに顕著な突起伸展がみられ、これはCD分子に取り込まれた生理活性物質の作用と考えられた。また、β-CDを用いた無血清培養を利用してラット脳から精製したコンドロイチン硫酸プロテオグリカン（CSPG）の作用を検討し、CSPGがグルタミン酸による神経細胞死を防止すること、弱いながら培養神経細胞の生存を維持する作用をもつことを示した。以上の結果から、この無血清培養法は神経生物学的研究において有用な分析系となりうることを指摘した。 en-copyright= kn-copyright= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name=岡本基 kn-aut-sei=岡本 kn-aut-mei=基 aut-affil-num=1 ORCID= en-aut-name=MoriShuji en-aut-sei=Mori en-aut-mei=Shuji kn-aut-name=森秀治 kn-aut-sei=森 kn-aut-mei=秀治 aut-affil-num=2 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=NitaNahoko kn-aut-sei=Nita kn-aut-mei=Nahoko aut-affil-num=3 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=NoyoriMaki kn-aut-sei=Noyori kn-aut-mei=Maki aut-affil-num=4 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=HiranoHumiko kn-aut-sei=Hirano kn-aut-mei=Humiko aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=School of Health Sciences, Okayama University affil-num=2 en-affil= kn-affil=School of Health Sciences, Okayama University affil-num=3 en-affil= kn-affil=Hiroshima Teishin Hosptal affil-num=4 en-affil= kn-affil=Hagiwara Central Hospital affil-num=5 en-affil= kn-affil=Kanebo Memorial Hospital en-keyword=β-cyclodextrins kn-keyword=β-cyclodextrins en-keyword=γ-cyclodextrins kn-keyword=γ-cyclodextrins en-keyword=serum-free culture kn-keyword=serum-free culture en-keyword=cortical neuron kn-keyword=cortical neuron en-keyword=chondroitin sulfate proteoglycan kn-keyword=chondroitin sulfate proteoglycan END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue= article-no= start-page=1 end-page=12 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=19930131 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Alcoholic Liver Disease kn-title=アルコール性肝障害 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Relationship between ethanol drinking and organs injury was reviewed and special emphasis was put on alcoholic liver disease. Consumption of alcoholic beverage expressed as ethanol per capita of adult in Japan increased 2.1 times in these 25 years and it is still increasing. Although the incidence of alcoholic liver disease in Japan also increased greatly during the above period, it seems likely that plateau level is coming because of genetically defined, unique type of alcohol metabolism in Japanese. Sex differences in susceptibility to alcohol were discussed. Among the six types of alcoholic liver disease, alcoholic liver fibrosis is relatively frequent in Japan. Mechanism of liver injury has been studied extensively. Alcohol itself is toxic but other factors such as dietary fat are also important. Biochemical and immunological markers of drinking were presented. As for the treatment, most patients especially in early stages of the disease well respond to alcohol withdrawal, but therapy of alcohol dependence in the background of the disease is very difficult requiring cooperative works of different kinds of specialists. en-copyright= kn-copyright= en-aut-name=EndoHiroshi en-aut-sei=Endo en-aut-mei=Hiroshi kn-aut-name=遠藤浩 kn-aut-sei=遠藤 kn-aut-mei=浩 aut-affil-num=1 ORCID= en-aut-name=NakataYasunari en-aut-sei=Nakata en-aut-mei=Yasunari kn-aut-name=中田安成 kn-aut-sei=中田 kn-aut-mei=安成 aut-affil-num=2 ORCID= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name=岡本基 kn-aut-sei=岡本 kn-aut-mei=基 aut-affil-num=3 ORCID= en-aut-name=MoriShuji en-aut-sei=Mori en-aut-mei=Shuji kn-aut-name=森秀治 kn-aut-sei=森 kn-aut-mei=秀治 aut-affil-num=4 ORCID= en-aut-name=OhtaTakeo en-aut-sei=Ohta en-aut-mei=Takeo kn-aut-name=太田武夫 kn-aut-sei=太田 kn-aut-mei=武夫 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=2 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=3 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=4 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=5 en-affil= kn-affil=岡山大学医療技術短期大学部看護学科 en-keyword=alcohol kn-keyword=alcohol en-keyword=liver kn-keyword=liver en-keyword=epidemiology kn-keyword=epidemiology en-keyword=genetic factors kn-keyword=genetic factors en-keyword=alcohol dependence kn-keyword=alcohol dependence END start-ver=1.4 cd-journal=joma no-vol=1 cd-vols= no-issue= article-no= start-page=91 end-page=97 dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=19910325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The Neurophysiological Implications of an Atypical Slow Negative Potential in Short Interval CNV　Paradigm kn-title=CNVの記録条件と非定型波形の生理学的意義 en-subtitle= kn-subtitle= en-abstract=CNVの記録条件を検討する目的で、健康若年成人49名（男12、女37）を対象にS(1)-S(2)+Rパラダイムを用いてCNVを記録した。S(1)-S(2)間隔2秒のほうが3秒よりも安定してCNVが記録されたが、刺激間隔とは無関係に26〜30％の試行で二峰性の陰性変動をもつ非定型な緩電位が記録された。この緩電位は同一被検者でも出現性が一貫せず、個体差よりも被検者の状態に依存していた。二峰性陰性波のうち、後期成分は準備電位に対応するものと思われたが、前期成分はS(1)後900〜1100msecにピークをもつ陰性波で、これまで報告されたCNVの要素波とは対応しなかった。また、この非定型CNVを示す被検者ではCNV出現が不安定で、SVRおよびパターンVEPの振幅が低い傾向があり、試行時の被検者の覚醒水準低下よるものではないかと考えられた。 kn-abstract=We recorded a slow negative potential from Cz (10/20 method) in 49 healthy students (12 male, 37 female, mean age 19.1) by a short interstumulus interval CNV paradigm. The interstimulus interval was 2 or 3 seconds, the warning stimulus presented at random or regular interval at 0.2 Hz. An atypical negative variation with two separated negative peaks was recorded in 26.0-30.6% trials regardless of interstimulus interval or modality of warning stimulus presentation, while a typical CNV was recorded in 32.0-59.2% of trials. No apparent negative variation was recorded in 14.3-18.4% in 2 seconds interstimulus interval, and 28-38% in 3 seconds interstimulus interval, showing that 2 seconds interval is better to get stable CNV recording than 3 seconds interval. The first negative wave of the atypical negative variation was 692-799msec in duration, but frequently prolonged to 1000msec or more in 3 seconds interval. It usually had negative peak around 900-1100msec, but sometimes around 1500msec. This features are different from any reported negative components of CNV. The second negative wave began 800-1200msec before second stimulus, and had its peak just before second stimulus, showing common features with readiness potential. The appearance of CNV was unsatble in the students in which the atypical negative variation was recorded in regular, 2 seconds intersitimulus interval, and the amplitude of slow vertex response and pattern reversal visual evoked potential was lower in thses students than in the students in which a typical CNV was recorded more than 3 times in total 4 times of trials. These findings indicate that the atypical variation observed in this study is due to a lowered arousal level or cortical neuronal activity, rather than a separated appearance of different components of CNV. en-copyright= kn-copyright= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name=岡本基 kn-aut-sei=岡本 kn-aut-mei=基 aut-affil-num=1 ORCID= en-aut-name=TeminoMasato en-aut-sei=Temino en-aut-mei=Masato kn-aut-name=手蓑正人 kn-aut-sei=手蓑 kn-aut-mei=正人 aut-affil-num=2 ORCID= en-aut-name=NakagawaNaohisa en-aut-sei=Nakagawa en-aut-mei=Naohisa kn-aut-name=中川尚久 kn-aut-sei=中川 kn-aut-mei=尚久 aut-affil-num=3 ORCID= en-aut-name=MoriHirokazu en-aut-sei=Mori en-aut-mei=Hirokazu kn-aut-name=森宏和 kn-aut-sei=森 kn-aut-mei=宏和 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=2 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=3 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=4 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 en-keyword=contingent negative variation kn-keyword=contingent negative variation en-keyword=atypical slow negative potential kn-keyword=atypical slow negative potential en-keyword=arousal level kn-keyword=arousal level en-keyword=slow vertex response kn-keyword=slow vertex response en-keyword=visual evoked potential kn-keyword=visual evoked potential END start-ver=1.4 cd-journal=joma no-vol=7 cd-vols= no-issue=1 article-no= start-page=17 end-page=21 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=19960830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Alteration of thermostable phosphatase activity after hydrophobic chromatography kn-title=疎水性クロマトグラフィに伴う耐熱性ホスタファーゼ活性の変動 en-subtitle= kn-subtitle= en-abstract=Thermostable phosphatase partially purified from thermophilic bacteria, Bacillus stearothermophilus, was chromatographed on Resource Iso hydrophobic resin. When linear reverse gradient elution with 1.5 M → 0 M ( NH(4))(2)SO(4) was performed, phosphatase was found to be eluted as latent form, which revealed dose-dependent inhibitory effect of (NH(4))(2)SO(4) on phosphatase. When various concentrations of (NH(4))(2)SO(4) were added into phosphatase reaction mixture, about 80% inhibition was observed in the presence of 0.15 M (NH(4))(2)SO(4). Acidification by adding (NH(4))(2)SO(4) was not responsible for this inhibition, because addition of (NH(4))(2)SO(4) solution which pH was previously adjusted to 9.0 showed same inhibitory effect. kn-abstract=耐熱性ホスファターゼを含んだBacillus stearothermophilus 粗酵素試料を、リソースIsoによる疎水性クロマトグラフィにかけ分離を行った。1.5M→0M 硫酸アンモニウムの直線逆濃度勾配によって溶出を行ったところ、ホスファターゼは不活性な形で溶出され、これは硫酸アンモニウムによる濃度依存的阻害に起因することが判明した。ホスタファーゼの反応混合液に種々の濃度の硫酸アンモニウムを添加したところ、0.15Mの硫酸アンモニウム存在下で約80％の阻害が認められた。加えて、この阻害作用は単に硫酸アンモニウムの添加によってpHが酸性側に傾くことによるものではないことも明らかとなった。 en-copyright= kn-copyright= en-aut-name=MoriShuji en-aut-sei=Mori en-aut-mei=Shuji kn-aut-name=森秀治 kn-aut-sei=森 kn-aut-mei=秀治 aut-affil-num=1 ORCID= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name=岡本基 kn-aut-sei=岡本 kn-aut-mei=基 aut-affil-num=2 ORCID= en-aut-name=NakataYasunari en-aut-sei=Nakata en-aut-mei=Yasunari kn-aut-name=中田安成 kn-aut-sei=中田 kn-aut-mei=安成 aut-affil-num=3 ORCID= en-aut-name=EndoHiroshi en-aut-sei=Endo en-aut-mei=Hiroshi kn-aut-name=遠藤浩 kn-aut-sei=遠藤 kn-aut-mei=浩 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=2 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=3 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=4 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 en-keyword=耐熱性ホスファターゼ (thermophilic phosphatase) kn-keyword=耐熱性ホスファターゼ (thermophilic phosphatase) en-keyword=疎水性クロマトグラフィ (hydrophobic chromatography) kn-keyword=疎水性クロマトグラフィ (hydrophobic chromatography) en-keyword=硫酸アンモニウム (ammonium sulfate) kn-keyword=硫酸アンモニウム (ammonium sulfate) en-keyword=濃度依存的阻害 (dose- dependent inhibition) kn-keyword=濃度依存的阻害 (dose- dependent inhibition) END start-ver=1.4 cd-journal=joma no-vol=6 cd-vols= no-issue= article-no= start-page=63 end-page=72 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=19960229 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=大腸菌を用いたフォスファカン（コンドロイチン硫酸プロテオグリカン）の融合コア蛋白の発現条件の検討 kn-title=Expression of Phosphacan, a chondroitin sulfate proteoglycan, core protein in Esherichia coli as a fusion protein with glutathione S-transferase en-subtitle= kn-subtitle= en-abstract=コンドロイチン硫酸プロテオグリカンの一種であるフォスフォアカンのコア蛋白の特異領域を、グルタチオン-S-トランスフェラーゼ（GST）との融合蛋白として大腸菌内で発現させ、その発現条件の検討を行った。胎生18日目のラット脳から抽出したmRNAを鋳型として、RT−PCRによって増幅したDNAフラグメントを発現ベクターに挿入した。PCRの為のプライマーは、BamH IとEcoR Iの酵素消化部位を5'末端に組み込んだものを用いた。GST融合蛋白を作製するために、PCR産物を一旦、T-Aクローニングシステムに組込み、その後プラスミド精製と制限酵素消化によって目的のフラグメントを切り出した後、pGEXベクターに再度組み込ませ、大腸菌（BL21）を形質転換した。形質転換した大腸菌（BL21）について、24〜48時間培養後に、37℃で保存したものと、37℃で24時間培養後に4℃で7〜10日間保存したものとの増殖曲線を比較したところ、両者に有意な差は認められなかった。融合蛋白の誘導に必要なイソプロピルチオ−β−D−ガラクトシド（IPTG）の添加の時期は、菌培養液の吸光度（550nm）が1.0以上の場合には融合蛋白に比べて他の大腸菌固有の蛋白の割合が相対的に増大してしまう為、また、吸光度が0.6以下では菌量が少ない為、吸光度が0.6〜1.0を示す時期に添加する方がより望ましいことがわかった。IPTGによる誘導後、融合蛋白の発現は6時間でプラトーに達し、その後は大腸菌固有の内在蛋白量の割合が相対的に増加した。以上の結果より、融合蛋白の発現を誘導するための至適条件は次のように決定された。1. IPTG誘導は、培養液の吸光度（550nm）が0.6〜1.0の際に開始する。2. IPTGによる誘導時間は、6時間とする。 kn-abstract=Optimal conditions for expressing a specific region of core protein of phosphacan, a chondroitin sulfate proteoglycan known as receptor type protein tyrosine phosphatase, as fusion protein with glutathione S-transferase (GST) in E.coli were examined. DNA fragments inserted into the expression vector (pGEX-4T-1) were amplified by RT-PCR using mRNA purified from E18 rat brain as template. Primers attached with BamH I or EcoR I restriction site on 5' end were used to amplify first strand cDNA by PCR. Before ligation into the pGEX-4T-1 for GST fusion protein, PCR products were once cloned using T-A cloning system because they were not directly ligated into the pGEX-4T-1. E.coli strain BL21 was transformed by pGEX-4T-1 ligated with restriction DNA fragment cut out from pCR II plasmid vector of T-A clonig system. The growth of transformed BL21 was not different between the colony incubated at 37℃ for 24-48h and the colony stored at 4℃ for 7-10 days after 24h incubation at 37℃. The desirable OD(550) of culture medium for inducing the expression of fusion protein by isopropylthio-β-D-galactoside (IPTG) was from 0.6 to 1.0, because expression of native E.coli proteins per ml of culture medium was increased relatively when IPTG was added at OD(550) more than 1.0. The expression of fusion protein reached plateau around 6h after the induction. Relative expression of native E.coli proteins per ml of culture medium increased thereafter. Therefore, it may be desirable to purify the fusion protein around 6h after the induction. en-copyright= kn-copyright= en-aut-name=ItoSekiko en-aut-sei=Ito en-aut-mei=Sekiko kn-aut-name=伊藤昔子 kn-aut-sei=伊藤 kn-aut-mei=昔子 aut-affil-num=1 ORCID= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name=岡本基 kn-aut-sei=岡本 kn-aut-mei=基 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 affil-num=2 en-affil= kn-affil=岡山大学医療技術短期大学部衛生技術学科 en-keyword=phosphacan (フォスファカン) kn-keyword=phosphacan (フォスファカン) en-keyword=glutathione S-transferase (グルタチオン-S-トランスフェラーゼ) kn-keyword=glutathione S-transferase (グルタチオン-S-トランスフェラーゼ) en-keyword=BL21 kn-keyword=BL21 en-keyword=IPTG kn-keyword=IPTG en-keyword=fusion protein (融合蛋白) kn-keyword=fusion protein (融合蛋白) END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=2 article-no= start-page=69 end-page=76 dt-received= dt-revised= dt-accepted= dt-pub-year=2000 dt-pub=20000324 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=An experimental study on the relation of T2-signal high intensity in MRI to histopathological changes in the kainic acid model of temporal lobe epilepsy in rats. kn-title=ラットにおけるカイニン酸けいれん発作重積後のMRI所見と病理所見の関係 en-subtitle= kn-subtitle= en-abstract=The relation of T2-signal high intensity areas observed in temporal lobe epilepsy to histopathological changes in limbic structures was examined in the rat kainic acid (KA) model of epilepsy. Male 8-week-old Sprague-Dawley rats were injected with 10mg/kg(i.p.) of KA or saline (control). Repetitive generalized convulsions (stage 3, or stage 4,5 seizures of amygdala kindled seizures) lasted for 3 to 4 hrs. were induced by KA injection in all rats. MRI was recorded on the day before the KA injection and 1, 2, 4, 8 weeks after the injection by fluid-attenuated inversion recovery method under deep pentobarbital anesthesia. Following the last MRI recording, rats were perfused with 4% paraformaldehyde (PFA) from left cardiac ventricle, post-fixed overnight in 4% PFA and brains were embedded in parafin. Coronal brain sections (6μm) were stained with cresyl violet, or mouse anti-GFAP antibody followed by biotinylated goat anti-mouse IgG and avidin-biotin-peroxidase (vectastain ABC kit). Irregularshaped moderate to severe high T2-signal areas were observed in bilateral piriform and entorhinal cortex in MRI. These high T2-signal areas were ovserved from 1 week after the KA injection to 8 weeks after the injection, and were more prominent in rats elicited stage 4 or 5 seizures than in rats elicited stage 3 seizures. Loss of pyramidal neurons and increased GFAP immunoreactivity were observed in piriform cortex, entorhinal cortex, CA1, subiculum, and hilus of dentate gyrus. The increase of GFAP immunoreactivity, but not the intensity of neuronal loss, in piriform and entorhinal cortex was almost correponded to the size and intensity of T2-signal. However, the increase of GFAP immunoreactivity in hippocampus was not detected as the increase of T2-signal in MRI. These findings indicate that astroglial reactions in piriform and entorhinal cortex are more sensitive to T2-weighted MRI than those in hippocampus. kn-abstract=側頭葉てんかんでは，てんかん焦点に一致してMRI T2高信号領域が見られ，FLAIR法でこれがより明瞭になるが，このMRI所見と病理組織学的変化との関係は必ずしもはっきりしていない。そこで，Sprague−Dawleyラットにカイニン酸（KA）でけいれん発作重積状態を起こし，経時的にMRIを記録するとともに，ニッスル染色，GFAP免疫染色での病理組織学的変化を調べて両者の関係について検討した。KA群では，MRIで1〜8週間後のいずれにおいてもpiriform cortexからentorhinal cortexにかけて不整形のT2高信号領域がみられたが，stage3のけいれん発作しか出現しなかったラットではstage4，5が出現したラットに比べて程度が弱かった。組織学的には，CA1，subiculum，piriform cortex，entorhinal cortexで神経細胞の消失，濃染細胞の増加と萎縮，GFAP免疫反応の増強が見られたが，piriform cortex，entorhinal cortexでの神経細胞消失の程度はT2信 号の程度と相関せず，GFAP免疫反応が増強した領域に一致して高信号がみられた。しかし，海馬のGFAP免疫反応増強はMRI所見に反映されず，これはMRIの解像度の限界にもよると考えられた。 en-copyright= kn-copyright= en-aut-name=SakiyamaJunko en-aut-sei=Sakiyama en-aut-mei=Junko kn-aut-name=ｱ山順子 kn-aut-sei=ｱ山 kn-aut-mei=順子 aut-affil-num=1 ORCID= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name=岡本基 kn-aut-sei=岡本 kn-aut-mei=基 aut-affil-num=2 ORCID= en-aut-name=KitamuraYoshihiro en-aut-sei=Kitamura en-aut-mei=Yoshihiro kn-aut-name=北村吉宏 kn-aut-sei=北村 kn-aut-mei=吉宏 aut-affil-num=3 ORCID= en-aut-name=YamadaNorihito en-aut-sei=Yamada en-aut-mei=Norihito kn-aut-name=山田了士 kn-aut-sei=山田 kn-aut-mei=了士 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部保健学科検査技術科学専攻 affil-num=2 en-affil= kn-affil=岡山大学医学部保健学科検査技術科学専攻 affil-num=3 en-affil= kn-affil=岡山療護センター affil-num=4 en-affil= kn-affil=岡山大学医学部医学科神経精神医学講座 en-keyword=カイニン酸 (kainic acid) kn-keyword=カイニン酸 (kainic acid) en-keyword=MRI kn-keyword=MRI en-keyword=FLAIR法 (FLAIR) kn-keyword=FLAIR法 (FLAIR) en-keyword=神経細胞死 (neuronal death) kn-keyword=神経細胞死 (neuronal death) en-keyword=GFAP kn-keyword=GFAP END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=19831231 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=正中離断による全汎化機序の検討 燃え上がり現象を用いた側頭部皮質焦点発作の実験的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=岡本基 kn-aut-sei=岡本 kn-aut-mei=基 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END