start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=2400078
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240704
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Unabsorbed Fecal Fat Content Correlates with a Reduction of Immunoglobulin a Coating of Gut Bacteria in High]Lard Diet]Fed Mice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Scope: Immunoglobulin A (IgA) selectively coats gut bacteria and contributes to regulatory functions in gastrointestinal inflammation and glucose metabolism. Excess intake of lard leads to decrease in the IgA coating of gut bacteria, although the underlying mechanisms remain unknown. This study validates how unabsorbed fat derived from a high-lard diet in the gut affects the IgA coating of bacteria, as assessed in mouse models using three types of dietary fat (lard, medium-, and long-chain triglycerides [MLCTs], and medium-chain triglycerides [MCTs]) exhibiting different digestibilities.
Methods and results: C57BL/6J mice are maintained on diets containing lard, MLCTs, or MCTs at 7% or 30% w/w for 10 weeks (n = 6 per group). The fecal fatty acid concentration is measured to quantify unabsorbed fat content. The ratio of IgA-coated bacteria to total bacteria (IgA coating ratio) in the feces is measured by flow cytometry. Compared to lard-fed mice, MLCT- and MCT-fed mice exhibit lower fecal concentrations of palmitic acid, stearic acid, and oleic acid and higher IgA coating ratios at both 7% and 30% dietary fat, and these parameters exhibit significant negative correlations.
Conclusion: Unabsorbed fat content in the gut may result in attenuated IgA coating of bacteria in high-lard diet-fed mice.
en-copyright=
kn-copyright=
en-aut-name=KatsumataEmiko
en-aut-sei=Katsumata
en-aut-mei=Emiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=TsurutaTakeshi
en-aut-sei=Tsuruta
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=SonoyamaKei
en-aut-sei=Sonoyama
en-aut-mei=Kei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=YoshidaTakashi
en-aut-sei=Yoshida
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=SasakiMio
en-aut-sei=Sasaki
en-aut-mei=Mio
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=TeraokaMao
en-aut-sei=Teraoka
en-aut-mei=Mao
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=WangTianyang
en-aut-sei=Wang
en-aut-mei=Tianyang
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=NishinoNaoki
en-aut-sei=Nishino
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=2
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=3
en-affil=Research Faculty of Agriculture, Hokkaido University
kn-affil=
affil-num=4
en-affil=TAIYO YUSHI Corporation
kn-affil=
affil-num=5
en-affil=TAIYO YUSHI Corporation
kn-affil=
affil-num=6
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=7
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=8
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=gut bacteria
kn-keyword=gut bacteria
en-keyword=immunoglobulin A
kn-keyword=immunoglobulin A
en-keyword=lard
kn-keyword=lard
END
start-ver=1.4
cd-journal=joma
no-vol=10
cd-vols=
no-issue=3
article-no=
start-page=226
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20230316
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Monitoring the Milk Composition, Milk Microbiota, and Blood Metabolites of Jersey Cows throughout a Lactation Period
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This study aimed to determine how milk composition, milk microbiota, and blood metabolites may change during the lactation period in Jersey cows. Milk and jugular blood samples were collected from eight healthy cows every other month from the beginning to the end of their lactation period. Samples of airborne dust were also collected to determine whether the cowshed microbiota could affect milk microbiota. Milk yield peaked in the first two months and gradually decreased as the lactation period progressed. Milk fat, protein, and solids-not-fat contents were low in the first month, and then increased during the middle and late lactation periods. In the first month, plasma non-esterified fatty acids (NEFA), haptoglobin (Hp), and aspartate transaminase (AST) levels were elevated, and high abundances of Burkholderiaceae and Oxalobacteraceae were observed in milk and airborne dust microbiota. The finding that contamination of the environmental microbiota in milk was coupled with elevated plasma NEFA, Hp, and AST levels indicated that impaired metabolic function during the early lactation period may increase the invasion of opportunistic bacteria. This study can affirm the importance of feeding and cowshed management and should provide a helpful addition to improving Jersey cow farming.
en-copyright=
kn-copyright=
en-aut-name=GathinjiPeter Kiiru
en-aut-sei=Gathinji
en-aut-mei=Peter Kiiru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=YousofiZabiallah
en-aut-sei=Yousofi
en-aut-mei=Zabiallah
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=AkadaKarin
en-aut-sei=Akada
en-aut-mei=Karin
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=WaliAjmal
en-aut-sei=Wali
en-aut-mei=Ajmal
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=NishinoNaoki
en-aut-sei=Nishino
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
affil-num=1
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=2
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=3
en-affil=Animal Products Research Group, Institute of Livestock and Grassland Science, National Agriculture and Research Organization
kn-affil=
affil-num=4
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=5
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=airborne dust
kn-keyword=airborne dust
en-keyword=blood metabolites
kn-keyword=blood metabolites
en-keyword=Jersey cows
kn-keyword=Jersey cows
en-keyword=microbiota
kn-keyword=microbiota
en-keyword=milk
kn-keyword=milk
END
start-ver=1.4
cd-journal=joma
no-vol=8
cd-vols=
no-issue=1
article-no=
start-page=10
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20211229
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Bacterial and Fungal Microbiota of Guinea Grass Silage Shows Various Levels of Acetic Acid Fermentation
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This study aimed to gain insights into the bacterial and fungal microbiota associated with the acetic acid fermentation of tropical grass silage. Direct-cut (DC, 170 g dry matter [DM]/kg) and wilted (WT, 323 g DM/kg) guinea grass were stored in a laboratory silo at moderate (25 degrees C) and high (40 degrees C) temperatures. Bacterial and fungal microbiota were assessed at 3 days, 1 month, and 2 months after ensiling. Lactic acid was the primary fermentation product during the initial ensiling period, and a high Lactococcus abundance (19.7-39.7%) was found in DC silage. After two months, the lactic acid content was reduced to a negligible level, and large amounts of acetic acid, butyric acid, and ethanol were found in the DC silage stored at 25 degrees C. The lactic acid reduction and acetic acid increase were suppressed in the DC silage stored at 40 degrees C. Increased abundances of Lactobacillus, Clostridium, and Wallemia, as well as decreased abundances of Saitozyma, Papiliotrema, and Sporobolomyces were observed in DC silages from day three to the end of the 2 month period. Wilting suppressed acid production, and lactic and acetic acids were found at similar levels in WT silages, regardless of the temperature and storage period. The abundance of Lactobacillus (1.72-8.64%) was lower in WT than in DC silages. The unclassified Enterobacteriaceae were the most prevalent bacteria in DC (38.1-64.9%) and WT (50.9-76.3%) silages, and their abundance was negatively related to the acetic acid content. Network analysis indicated that Lactobacillus was involved in enhanced acetic acid fermentation in guinea grass silage.
en-copyright=
kn-copyright=
en-aut-name=HouJianjian
en-aut-sei=Hou
en-aut-mei=Jianjian
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=NishinoNaoki
en-aut-sei=Nishino
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=2
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=bacteria
kn-keyword=bacteria
en-keyword=fungi
kn-keyword=fungi
en-keyword=silage
kn-keyword=silage
en-keyword=storage temperature
kn-keyword=storage temperature
en-keyword=tropical grass
kn-keyword=tropical grass
END
start-ver=1.4
cd-journal=joma
no-vol=7
cd-vols=
no-issue=1
article-no=
start-page=1
end-page=12
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=20210114
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Cecum microbiota in rats fed soy, milk, meat, fish, and egg proteins with prebiotic oligosaccharides
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Diet is considered the most influential factor in modulating the gut microbiota but how dietary protein sources differ in their modulatory effects is not well understood. In this study, soy, meat (mixture of beef and pork), and fish proteins (experiment 1) and soy, milk (casein), and egg proteins (experiment 2) were fed to rats with cellulose (CEL) and raffinose (RAF); the microbiota composition and short-chain fatty acid concentration in the cecum were determined. Egg protein feeding decreased the concentration of acetic acid and the richness and diversity of the cecum microbiota. RAF feeding increased the concentrations of acetic and propionic acids and decreased the richness and diversity of the cecum microbiota. When fed with CEL, the abundance of Ruminococcaceae and Christensenellaceae, Akkermansiaceae and Tannerellaceae, and Erysipelotrichaceae enhanced with soy protein, meat and fish proteins, and egg protein, respectively. The effects of dietary proteins diminished with RAF feeding and the abundance of Bifidobacteriaceae, Erysipelotrichaceae, and Lachnospiraceae increased and that of Ruminococcaceae and Christensenellaceae decreased regardless of the protein source. These results indicate that, although the effect of prebiotics is more robust and distinctive, dietary protein sources may influence the composition and metabolic activities of the gut microbiota. The stimulatory effects of soy, meat, and egg proteins on Christensenellaceae, Akkermansiaceae, and Erysipelotrichaceae deserve further examination to better elucidate the dietary manipulation of the gut microbiota.
en-copyright=
kn-copyright=
en-aut-name=SivixaySouliphone
en-aut-sei=Sivixay
en-aut-mei=Souliphone
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=BaiGaowa
en-aut-sei=Bai
en-aut-mei=Gaowa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=TsurutaTakeshi
en-aut-sei=Tsuruta
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=NishinoNaoki
en-aut-sei=Nishino
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
affil-num=1
en-affil=Department of Animal Science, Graduate School of Life and Environmental Science, Okayama University
kn-affil=
affil-num=2
en-affil=Department of Animal Science, Graduate School of Life and Environmental Science, Okayama University
kn-affil=
affil-num=3
en-affil=Department of Animal Science, Graduate School of Life and Environmental Science, Okayama University
kn-affil=
affil-num=4
en-affil=Department of Animal Science, Graduate School of Life and Environmental Science, Okayama University
kn-affil=
en-keyword=diet
kn-keyword=diet
en-keyword=gut
kn-keyword=gut
en-keyword=microbiota
kn-keyword=microbiota
en-keyword=protein
kn-keyword=protein
en-keyword=prebiotics
kn-keyword=prebiotics
END
start-ver=1.4
cd-journal=joma
no-vol=33
cd-vols=
no-issue=11
article-no=
start-page=1858
end-page=1865
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20191224
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=An investigation of seasonal variations in the microbiota of milk, feces, bedding, and airborne dust
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Objective
The microbiota of dairy cow milk varies with the season, and this accounts in part for the seasonal variation in mastitis-causing bacteria and milk spoilage. The microbiota of the cowshed may be the most important factor because the teats of a dairy cow contact bedding material when the cow is resting. The objectives of the present study were to determine whether the microbiota of the milk and the cowshed vary between seasons, and to elucidate the relationship between the microbiota.
Methods
We used 16S rRNA gene amplicon sequencing to investigate the microbiota of milk, feces, bedding, and airborne dust collected at a dairy farm during summer and winter.
Results
The seasonal differences in the milk yield and milk composition were marginal. The fecal microbiota was stable across the two seasons. Many bacterial taxa of the bedding and airborne dust microbiota exhibited distinctive seasonal variation. In the milk microbiota, the abundances of Staphylococcaceae, Bacillaceae, Streptococcaceae, Microbacteriaceae, and Micrococcaceae were affected by the seasons; however, only Micrococcaceae had the same seasonal variation pattern as the bedding and airborne dust microbiota. Nevertheless, canonical analysis of principle coordinates revealed a distinctive group comprising the milk, bedding, and airborne dust microbiota.
Conclusion
Although the milk microbiota is related to the bedding and airborne dust microbiota, the relationship may not account for the seasonal variation in the milk microbiota. Some major bacterial families stably found in the bedding and airborne dust microbiota, e.g., Staphylococcaceae, Moraxellaceae, Ruminococcaceae, and Bacteroidaceae, may have greater influences than those that varied between seasons.
en-copyright=
kn-copyright=
en-aut-name=NguyenThuong Thi
en-aut-sei=Nguyen
en-aut-mei=Thuong Thi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=WuHaoming
en-aut-sei=Wu
en-aut-mei=Haoming
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=NishinoNaoki
en-aut-sei=Nishino
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
affil-num=1
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=2
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=3
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=Cowshed
kn-keyword=Cowshed
en-keyword=Dairy Cow
kn-keyword=Dairy Cow
en-keyword=Microbiota
kn-keyword=Microbiota
en-keyword=Milk
kn-keyword=Milk
en-keyword=Season
kn-keyword=Season
END
start-ver=1.4
cd-journal=joma
no-vol=8
cd-vols=
no-issue=9
article-no=
start-page=1334
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=20200901
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Bacterial and Fungal Microbiota Associated with the Ensiling of Wet Soybean Curd Residue under Prompt and Delayed Sealing Conditions
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Wet soybean curd residue (SCR) obtained from two tofu factories (F1 and F2) was anaerobically stored with or without added beet pulp (BP). Sealing was performed on the day of tofu production (prompt sealing (PS)) or 2 days after SCR was piled and unprocessed (delayed sealing (DS)). Predominant lactic acid fermentation was observed regardless of the sealing time and BP addition.Acinetobacterspp. were the most abundant (>67%) bacteria in pre-ensiled SCR, regardless of the factory and sealing time. In PS silage, the abundances of typical lactic acid-producing bacteria, such asLactobacillus,Pediococcus, andStreptococcusspp. reached >50%. In DS silage,Acinetobacterspp. were the most abundant in F1 products, whereasBacillusspp. were the most abundant in long-stored F2 products. The fungal microbiota were highly diverse. AlthoughCandida,Aspergillus,Cladosporium,Hannaella, andWallemiaspp. were found to be the most abundant fungal microbiota, no specific genera were associated with factory, sealing time, or fermentation products. These results indicated that owing to preceding processing, including heating, distinctive microbiota may have participated in the ensiling of wet by-products. Lactic acid fermentation was observed even in DS silage, and an association ofBacillusspp. was suggested.
en-copyright=
kn-copyright=
en-aut-name=WaliAjmal
en-aut-sei=Wali
en-aut-mei=Ajmal
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=NishinoNaoki
en-aut-sei=Nishino
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=2
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=amplicon sequencing
kn-keyword=amplicon sequencing
en-keyword=bacteria
kn-keyword=bacteria
en-keyword=fungi
kn-keyword=fungi
en-keyword=silage
kn-keyword=silage
en-keyword=soybean curd residue
kn-keyword=soybean curd residue
END
start-ver=1.4
cd-journal=joma
no-vol=39
cd-vols=
no-issue=3
article-no=
start-page=188
end-page=196
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=2020
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Cyclic nigerosylnigerose ameliorates DSS-induced colitis with restoration of goblet cell number and increase in IgA reactivity against gut microbiota in mice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Cyclic nigerosylnigerose (CNN) is a cyclic oligosaccharide. Oral administration of CNN promotes immunoglobulin A (IgA) secretion in the gut. IgA is a major antibody secreted into the gut and plays a crucial role in suppressing gut inflammation due to commensal gut microbiota. To investigate the effect of administration of CNN to promote IgA secretion on gut inflammation, experimental colitis was induced with dextran sulfate sodium (DSS) in Balb/c mice after 6 weeks of CNN pre-feeding. The severity of colitis was evaluated based on a disease activity index (DAI), the gene expression of inflammatory cytokines, and a histological examination. The CNN-treated mice with DSS-induced colitis (CNN-DSS group) showed significantly lower DAI scores and mRNA levels of interleukin-1 compared with the CNN-untreated mice with DSS-induced colitis (DSS group). Histological examination of the colon revealed that the pathological score was significantly lower in the CNN-DSS group compared with the DSS group due to the reduced infiltration of immune cells. The number of goblet cells was significantly higher in the CNN-DSS group compared with the DSS group. The IgA concentration and the ratio of microbiota coated with IgA were evaluated in the cecal content. Although there was no difference in the IgA concentration among groups, a higher proportion of cecal microbiota were coated with IgA in the CNN-DSS group compared with that in the DSS group. These results suggest that CNN might preserve goblet cells in the colon and promote IgA coating of gut microbiota, which synergistically ameliorate gut inflammation in mice with DSS-induced colitis.
en-copyright=
kn-copyright=
en-aut-name=TsurutaTakeshi
en-aut-sei=Tsuruta
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=KatsumataEmiko
en-aut-sei=Katsumata
en-aut-mei=Emiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=MizoteAkiko
en-aut-sei=Mizote
en-aut-mei=Akiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=JianHou Jian
en-aut-sei=Jian
en-aut-mei=Hou Jian
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=MuhomahTeresia Aluoch
en-aut-sei=Muhomah
en-aut-mei=Teresia Aluoch
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=NishinoNaoki
en-aut-sei=Nishino
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
affil-num=1
en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=2
en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=3
en-affil=Hayashibara Co., Ltd.
kn-affil=
affil-num=4
en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=5
en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=6
en-affil=Laboratory of Animal Nutrition, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=oligosaccharide
kn-keyword=oligosaccharide
en-keyword=DSS-induced colitis
kn-keyword=DSS-induced colitis
en-keyword=goblet cell
kn-keyword=goblet cell
en-keyword=gut microbiota
kn-keyword=gut microbiota
en-keyword=immunoglobulin A
kn-keyword=immunoglobulin A
END
start-ver=1.4
cd-journal=joma
no-vol=9
cd-vols=
no-issue=12
article-no=
start-page=1007
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20191121
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=The Relationship between Uterine, Fecal, Bedding, and Airborne Dust Microbiota from Dairy Cows and Their Environment: A Pilot Study
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Simple Summary After calving, dairy cows face the risk of negative energy balance, inflammation, and immunosuppression, which may result in bacterial infection and disruption of the normal microbiota, thus encouraging the development of metritis and endometritis. This study characterized uterine, fecal, bedding, and airborne dust microbiota from postpartum dairy cows and their environment during summer and winter. The results clarify the importance of microbiota in cowshed environments, i.e., bedding and airborne dust, in understanding the postpartum uterine microbiota of dairy cows.
Abstract The aim of this study was to characterize uterine, fecal, bedding, and airborne dust microbiota from postpartum dairy cows and their environment. The cows were managed by the free-stall housing system, and samples for microbiota and serum metabolite assessment were collected during summer and winter when the cows were at one and two months postpartum. Uterine microbiota varied between seasons; the five most prevalent taxa were Enterobacteriaceae, Moraxellaceae, Ruminococcaceae, Staphylococcaceae, and Lactobacillaceae during summer, and Ruminococcaceae, Lachnospiraceae, Bacteroidaceae, Moraxellaceae, and Clostridiaceae during winter. Although Actinomycetaceae and Mycoplasmataceae were detected at high abundance in several uterine samples, the relationship between the uterine microbiota and serum metabolite concentrations was unclear. The fecal microbiota was stable regardless of the season, whereas bedding and airborne dust microbiota varied between summer and winter. With regards to uterine, bedding, and airborne dust microbiota, Enterobacteriaceae, Moraxellaceae, Staphylococcaceae, and Lactobacillaceae were more abundant during summer, and Ruminococcaceae, Lachnospiraceae, Bacteroidaceae, and Clostridiaceae were more abundant during winter. Canonical analysis of principal coordinates confirmed the relationship between uterine and cowshed microbiota. These results indicated that the uterine microbiota may vary when the microbiota in cowshed environments changes.
en-copyright=
kn-copyright=
en-aut-name=NguyenThuong T.
en-aut-sei=Nguyen
en-aut-mei=Thuong T.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=MiyakeAyumi
en-aut-sei=Miyake
en-aut-mei=Ayumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=TranTu T. M.
en-aut-sei=Tran
en-aut-mei=Tu T. M.
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=TsurutaTakeshi
en-aut-sei=Tsuruta
en-aut-mei=Takeshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=NishinoNaoki
en-aut-sei=Nishino
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
affil-num=1
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=2
en-affil=Okayama Prefecture Livestock Research Institute
kn-affil=
affil-num=3
en-affil=Faculty of Agriculture and Food Technology, Tien Giang University
kn-affil=
affil-num=4
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=5
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=cowshed
kn-keyword=cowshed
en-keyword=environment
kn-keyword=environment
en-keyword=microbiota
kn-keyword=microbiota
en-keyword=uterus
kn-keyword=uterus
END
start-ver=1.4
cd-journal=joma
no-vol=91
cd-vols=
no-issue=6
article-no=
start-page=2380
end-page=2387
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=20080626
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Ensiling of soybean curd residue and wet brewers grains with or without other feeds as a total mixed ration
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
Wet brewers grains and soybean curd residue were stored in laboratory-scale silos without (BG and SC silages, respectively) or with other ingredients as total mixed rations (BGT and SCT silages, respectively). Silages were opened after 14 and 56 d, and microbial counts, fermentation products, and aerobic stability were determined. Denaturing gradient gel electrophoresis was carried out to examine bacterial communities, and several bacteria that appeared to be involved in fermentation were identified. Lactic acid content was greater in SCT than in BGT silage, but lower in SC than in BG silage. Ethanol content was greater in BG than in SC regardless of silage type. Aerobic deterioration occurred promptly in ensiling materials (nonensiled by-products and total mixed ration mixtures) and in silages stored alone; however, SCT and BGT silages resisted deterioration and no heating was found for more than 5.5 d regardless of storage period. Silages were stable even with high yeast populations at silo opening, whereas prolonged ensiling decreased yeast counts in the 2 total mixed ration silages. The denaturing gradient gel electrophoresis profiles appeared similar between SCT and BGT silages but not between SC and BG silages. Weissella spp. and Lactobacillus brevis were common in aerobically stable SCT and BGT silages, and Lactobacillus buchneri was detected only in BGT silage. Both L. brevis and L. buchneri were found in silage but not in ensiling materials. Several other lactic acid bacteria were also identified in SCT and BGT silages, but did not appear to be related to fermentation and aerobic stability.
en-copyright= kn-copyright= en-aut-name=WangF. en-aut-sei=Wang en-aut-mei=F. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NishinoNaoki en-aut-sei=Nishino en-aut-mei=Naoki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=Department of Biomolecular Science, Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Department of Biomolecular Science, Graduate School of Natural Science and Technology, Okayama University en-keyword=by-product kn-keyword=by-product en-keyword=denaturing gradient gel electrophoresis; silage kn-keyword=denaturing gradient gel electrophoresis; silage en-keyword=total mixed ration kn-keyword=total mixed ration END