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ID 30947
JaLCDOI
フルテキストURL
著者
Khan, Rasel Laboratory of Protein Function, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Takahashi, Eizo Laboratory of Protein Function, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Nakura, Hironori Laboratory of Protein Function, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Ansaruzzaman, Mohammad Laboratory Sciences Division, ICDDR, B (International Centre for Diarrhoeal Diseases Research, Bangladesh), Centre for Health and Population Research
Banik, Sukalyani Laboratory of Protein Function, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Ramamurthy, Thandavarayan Collaborative Research Center of Okayama University for Infectious Diseases in India
Okamoto, Keinosuke Laboratory of Protein Function, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
抄録

Aeromonas are water-borne pathogens. They are halotolerant, which means that they can survive in environments whose salt content corresponds to that of seawater (3.0% NaCl). However, the presence of Aeromonas in seawater is extremely rare compared with that in river water. In this study, we tested the ability of Aeromonas sobria to produce toxins in river water and seawater. First, we cultured A. sobria on skim milk agar plates supplemented with either river water (SARW) or seawater (SASW). The bacteria grew on both plates. A clear zone around the bacteria was generated in SARW. However, such a zone was not observed in SASW, suggesting that proteases were not generated in SASW. Subsequently, we cultured A. sobria in a nutrient broth supplemented with either river water (NRW) or with seawater (NSW), and examined the protease activity of their culture supernatants. The protease activity of the culture supernatant from NSW was extremely low compared to that from NRW. The immunoblotting analysis showed that serine protease (ASP) was not produced by the culture in NSW. By contrast, aerolysin-like hemolysin was produced in all conditions examined in this study. This indicates that the salinity of water is deeply involved in the production of ASP by A. sobria.

キーワード
Aeromonas
water
toxin
salinity
Amo Type
Original Article
発行日
2008-12
出版物タイトル
Acta Medica Okayama
62巻
6号
出版者
Okayama University Medical School
開始ページ
363
終了ページ
371
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
Web of Sience KeyUT